Skip to main content
SpringerLink
Log in

Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction

  • Published:
MRS Online Proceedings Library Aims and scope

Abstract

Polymerase Chain Reaction (PCR) is a complicated gene amplification process using Mg2+ as an assistant factor of Taq enzyme. Influence of single-walled carbon nanotube on that reaction was investigated with scanning electron microscope (SEM), transmission electron microscope TEM) and quantitative PCR product analysis. Results showed that adding single-walled carbon nanotubes in the reaction liquid with a concentration of less than 3 νg/νl increase the amounts of PCR products. But more carbon nanotubes cause a great reduction of the PCR products. Similar effects were observed in the reaction without Mg2+. Possible reason could be the aggregation of reaction components caused by small amount of additive carbon nanotubes, which increases reaction probability. Our observations suggest that small amount of carbon nanotube could be used as an assistant factor to improve the PCR reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. L. Borresen, Tidsskr Nor Laegeforen 113, 3668–3669 (1993).

    CAS  Google Scholar 

  2. R. J. Chen, Y. G. Zhang, D. W. Wang and H. J. Dai. J. Am. Chem. Soc.123, 3838–3839 (2001).

    Article  CAS  Google Scholar 

  3. C. Dwyer, M. Guthold, M. Falvo, S. Washburn, R. Superfine and D. Erie, Nanotechnology 13, 601–604 (2002).

    Article  CAS  Google Scholar 

  4. Vincenzo Iordi and Nan Yao. J. Mater. Res. 15, 2770–2779 (2000).

    Article  Google Scholar 

  5. Z. J. Guo, P. J. Sadler, and S. C. Tsang., Adv. Mater. 10, 701–703 (1998).

    Article  CAS  Google Scholar 

  6. C. V. Nguyen, L. Delzeit, A. M. Cassell, J. Li, J. Han, and M. Meyyappan., Nano Letters 2, 1079–1081 (2002).

    Article  CAS  Google Scholar 

  7. T. K. Mandal, M. S. Fleming, and D. R.Walt., Nano Letters 2, 3–7 (2002).

    Article  Google Scholar 

  8. M. Terrones, W. K. Hsu, A. Schilder, H. Terrones, N. Grobert, J. P. Hare, Y. Q. Zhu, M. Schwoerer, K. Prassides, H. K. Kroto, D. R. M. Walton, Appl. Phys. A 66, 307–317 (1998).

    Article  Google Scholar 

  9. D. Cui and H. Gao. Biotech. Prog. 2003.

  10. H. Gao,Y. Kong, D. Cui and C. S. Ozkan., Nano Letters 3, 471(2003).

    Article  CAS  Google Scholar 

  11. S. A. Chisholm, E. L. Teare, B. Patel, R. J. Owen, Lett. Appl. Microbiol. 35, 42–46 (2002).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569, Stuttgart, Germany

    Daxiang Cui, Furong Tian, Yong Kong, Cengiz S. Ozkan, Igor Titushikin & Huajian Gao

  2. Department of Mechanical Engineering, University of California, 92521-0425, Riverside, CA, USA

    Cengiz S. Ozkan

Authors
  1. Daxiang Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Furong Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yong Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cengiz S. Ozkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Igor Titushikin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Huajian Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cui, D., Tian, F., Kong, Y. et al. Effects of Single-Walled Carbon Nanotube on Polymerase Chain Reaction. MRS Online Proceedings Library 773, 23 (2003). https://doi.org/10.1557/PROC-773-N2.3

Download citation

  • Published:

  • DOI: https://doi.org/10.1557/PROC-773-N2.3

Search

Navigation