Skip to main content
SpringerLink
Log in

Analytical and Structural Electron Microscopy of Metal-Cell Interactions.

  • Published:
MRS Online Proceedings Library Aims and scope

Abstract

Human gingival fibroblasts have been grown in culture on thin (10–50nm) substrates of nickel, chromium and titanium and examined by structural and analytical transmission electron microscopy. Cells grown on titanium show no signs of metal uptake and have morphologies very similar to controls grown on carbon substrates. However, cells grown on nickel and chromium show significant metal uptake and some structural abnormalities. The majority of the ingested nickel and chromium appears to be associated with nucleic acids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. R. Alderson, N. S. Rattan and L. Bidstrup, Brit. J. Ind. Med. 38, 117 (1981).

    CAS  Google Scholar 

  2. A. Chovil, R. B. Sunderland and M. Halliday, Brit. J. Ind. Med. 38, 327 (1981).

    CAS  Google Scholar 

  3. F. W. Sunderman, R. M. Maenza and S. M. Hopfer, J. Environ. Pathol. Toxicol. 2, 1511 (1979).

    CAS  Google Scholar 

  4. C. P. Flessel, in ‘Trace elements in health and disease’ edited by N. Kharasch (New York, Raven Press, 1980) p. 109.

  5. P. Thomsen and L E. Ericson, Biomaterials, 6, 421 (1985).

    Article  CAS  Google Scholar 

  6. F. P. Ottensmeyer, Science 215, 461 (1982). 215, 461.

  7. F. P. Ottensmeyer, Annals N.Y. Acad. Sci., 483, 339 (1987).

    Article  Google Scholar 

  8. F. P. Ottensmeyer, J. Urtrastruct. Res., 88, 135 (1984).

    Article  Google Scholar 

  9. R. B. Ciccarelli and K. E. Wetterhahn, Cancer Res., 42, 3544 (1982).

    CAS  Google Scholar 

  10. H. Ono, O. Wada and T. Ono, J. Toxicol. Environ. Health, 8, 947 (1981).

    Article  CAS  Google Scholar 

  11. J. DiPaolo and B. Casto, Cancer Res., 39, 1008. (1979).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Metallurgy and Materials Science, University of Toronto, 184 College Street, M5S 1A4, Toronto, Canada

    Neil A. Coombs, Robert M. Pilliar & George C. Weatherly

Authors
  1. Neil A. Coombs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert M. Pilliar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. George C. Weatherly
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coombs, N.A., Pilliar, R.M. & Weatherly, G.C. Analytical and Structural Electron Microscopy of Metal-Cell Interactions.. MRS Online Proceedings Library 110, 349–353 (1987). https://doi.org/10.1557/PROC-110-349

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1557/PROC-110-349

Search

Navigation