Journal of Gastrointestinal Disorders and Liver Function

Objective: HIV-infected patients are at an increased risk of developing Hepatic Steatosis (HS), which may progress to nonalcoholic steatohepatitis. The aim of this study was to evaluate HS in Japanese HIV-infected patients using a novel technique called Controlled Attenuation Parameter (CAP). Methods: Sixty HIV-infected outpatients were included in this study and underwent transient elastography with CAP. Significant Hepatic Steatosis (SHS) was defined using the previously established CAP cut-off of 232dB/m. The relationships between SHS and blood chemistry, HBV/HCV co-infection and antiretroviral drugs were analyzed in HIV-infected patients. Results: The mean CAP level was 228dB/m. SHS was detected in 25 patients (42.3%). In the univariate analysis, the percentage of patients with SHS increased with the levels of triglyceride, fasting plasma glucose, HbA1c and BMI. SHS was observed less frequently among those who had been treated with oral tenofovir for 2 years or longer. Neither Hepatitis B surface Antigen (HBsAg) -positive rates, the duration for which patients were positive for HBsAg, HCV antibody-positive rates, nor the duration for which patients were positive for HCV RNA were associated with SHS. In a multivariate analysis, the only factor associated with SHS was BMI (adjusted odds ratio 1.727, 95% CI: 1.095 2.724; P = 0.019). Conclusion: The prevalence of SHS was markedly higher as 42.3% among HIV-infected patients and higher BMI in Japanese individuals was associated with SHS. *Corresponding author: Noboru Hirashima, MD/Ph.D, Department of Gastroenterology, National Hospital Organization, Nagoya Medical Center, Sannomaru, Naka, Nagoya 460-0001, Japan. Tel: +81-52-951-1111; Fax: +81-52-951-0664; E-mail: hirasima@nnh.hosp.go.jp Received Date: November 5, 2015 Accepted Date: November 30, 2015 Published Date: December 2, 2015 Citation: Hirashima, N., et al. Hepatic Steatosis, Measured by Transient Elastography with Controlled Attenuation Parameter, Is Highly Prevalent in Japanese HIV-Infected Patients. (2015) J Gastrointest Disord Liver Func 1(2): 17. J Gastrointest Disord Liver Func | volume 1: issue 2 Introduction Hepatic steatosis (HS) is one of the most common causes of liver disease in human immunodeficiency virus (HIV)-infected patients as well as in the general population[1,2]. HS is associated with an increased risk of liver inflammation and fibrosis, “so called” Nonalcoholic Steatohepatitis (NASH), a recognized cause of Liver Cirrhosis (LC) and Hepatocellular Carcinoma (HCC). HS in HIV-infected patients may progress to NASH, LC and HCC. The prevalence of HS and its risk factors in HIV-infected patients currently remain unclear[3]. HS rates in HIV-infected patients have been estimated based on the findings of previously conducted liver biopsy studies, which mostly involved HIV/HCV-co-infected patients. Liver biopsy is an invasive technique and sampling errors are common[4-6]. A new non-invasive assessment of HS through the measurement of controlled attenuation parameter (CAP) using the Copyrights: © 2015 Hirashima, N. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License. 1 1Department of Gastroenterology, National Hospital Organization Nagoya Medical Center, Nagoya, Japan 2Department of Infectious Diseases and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan 3Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan 4Department of Clinical Trials and Research, National Hospital Organization Nagoya Medical Center, Nagoya, Japan


Introduction
Hepatic steatosis (HS) is one of the most common causes of liver disease in human immunodeficiency virus (HIV)-infected patients as well as in the general population [1,2] . HS is associated with an increased risk of liver inflammation and fibrosis, "so called" Nonalcoholic Steatohepatitis (NASH), a recognized cause of Liver Cirrhosis (LC) and Hepatocellular Carcinoma (HCC). HS in HIV-infected patients may progress to NASH, LC and HCC. The prevalence of HS and its risk factors in HIV-infected patients currently remain unclear [3] . HS rates in HIV-infected patients have been estimated based on the findings of previously conducted liver biopsy studies, which mostly involved HIV/HCV-co-infected patients. Liver biopsy is an invasive technique and sampling errors are common [4][5][6] . A new non-invasive assessment of HS through the measurement of controlled attenuation parameter (CAP) using the 2 transient elastography (M probe) (FibroScan®; Echosens, Paris, France) has been developed. This novel technique estimates the presence of Significant Hepatic Steatosis (SHS) based on more than 10% of hepatocytes being detected in a liver biopsy sample [7]. Therefore, the aim of the present study was to evaluate SHS in Japanese HIV-infected patients using CAP [8].

Materials and Methods
Between January 2014 and May 2015, all HIV-infected outpatients in the Department of Infectious Diseases and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, were included in this study. They provided written informed consent to participate in this study and underwent transient elastography. CAP measurements were performed by an experienced practitioner at the Department of Gastroenterology, National Hospital Organization Nagoya Medical Center, Nagoya, using FibroScan®. CAP is an estimate of total ultrasonic attenuation and expressed in dB/m. In a previous study, examinations with ten successful shots, an interquartile range (IQR) for liver stiffness of less than 30% of the median value, and a success rate of at least 60%, were considered as reliable [9] and included in this study.
Sasso M, et al. [8] previously reported the CAP cut-off that defines SHS by presenting at least 10% hepatocytes to be 238dB/m. But, Masaki K, et al. [10] identified the CAP cut-off for defining SHS in Japanese patients as 232dB/m. Therefore, we herein selected 232dB/m as the CAP cut-off.
This study was designed in accordance with the Helsinki declaration and approved by the Ethics Committee of the National Hospital Organization Nagoya Medical Center.

Statistical Analysis
The primary outcome was the presence of SHS, defined as a CAP value equal to or greater than 232dB/m. The relationships between the following factors and SHS were analyzed: age, sex, race, rate of HIV infection, alcohol intake, tobacco consumption, CD4+ cell count, HIV RNA viral load, use of Antiretroviral Therapy (ART), AST, ALT, γGTP, platelet count, triglycerides, total cholesterol, LDL cholesterol, HDL cholesterol, fasting plasma glucose, HbA1c, Hepatitis B surface antigen (HBsAg), HBeAg, HBeAb, HBV DNA, the HBV genotype, HCV Ab, HCV RNA, the HCV genotype, BMI, waist circumference and Liver Stiffness Measurement (LSM). Continuous variables were expressed as a median (minimum-maximum) or mean (standard deviation, SD). Categorical variables were expressed as numbers (percentage). Factors associated with the primary outcome in logistic regression analysis with a P-value less than 0.05 were considered to be significant. Statistical analyses were performed using the SPSS 22 statistical software package (SPSS, Chicago, Illinois, USA).
Fifty-eight patients (97%) were treated with ART, with 33 (55%) showing undetectable HIV RNA levels. The distribution of antiretroviral drugs administered to patients is summarized in [Tables 2-3].  .001) were associated with SHS. HBsAg-positive rates, the duration for which patients were positive for HB-sAg, HCV Ab-positive rates, and the duration for which patients were positive for HCV RNA were not associated with SHS. LSM was also not associated with SHS. Relationships with SHS identified by a univariate analysis are summarized in [ Table 4]. The relationship between the antiretroviral drugs administered to our patients and SHS is shown in . The administration or not of antiretroviral drugs was not associated with SHS. The relationship between antiretroviral drug exposure for less than two years and equal to or greater than two years and SHS is shown in [Table -3]. The incidence of SHS decreased only when administering oral Tenofovir for 2 years or longer (odds ratio 0.210, 95%CI: 0.054-0.810; P=0.023).
A multivariate analysis revealed that the only factor associated with SHS was BMI . Waist circumference and HbA1c were excluded, because these were considered to present same aspects with BMI and fasting plasma glucose. BMI was the only predictor of SHS (per unit increase, adjusted odds ratio 1.727, 95%CI: 1.095-2.724; P=0.019)

Discussion
SHS, estimated to contain more than 10% of hepatocytes in liver biopsy samples and CAP of at least 232dB/m, was detected in 25 patients (42.3%) in the present study. The prevalence of HS in the general population in Western countries was previously reported to be approximately 20-30%; 30% in USA and 25% in Italy. In Japan, the prevalence of HS has been increasing and was reported to be 29.7% between 2009 and 2010 [2]. CAP measurements revealed that the prevalence of SHS was higher among HIV-infected patients than in the general population [11]. Macias J, et al. found that the prevalence of SHS among HIV-infected patients in Spain, as measured by CAP, was 40%, which is consistent with the results of the present study. A survey to estimate HS using transient elastography with CAP was recently performed on various hepatic disorders [12][13][14][15]. However, these surveys are rarely conducted on HIV-infected patients [16]. Previous studies demonstrated that it was possible to accurately detect SHS using a CAP cut-off of 238dB/m in Western countries and 232dB/m in Japan. The prevalence of HS and its risk factors in HIV-infected patients have not yet been clarified [8,10,15]. The previously reported prevalence of HS in HIV-infected patients was mostly derived from biopsy studies involving HIV/HCV-co-infected patients. Liver biopsy is an invasive technique and sampling errors are common [4][5][6]. The prevalence of HS in HIV/HCV-co-infected patients are known to be approximately 50% [17] however, few studies have examined HS in HIV-infected patients without HCV. In some cohort studies of HIV-infected patients, the prevalence of HS diagnosed using abdominal Ultrasound (US) was 31-53% [6,18].The prevalence of HS in HIV-infected patients without hepatitis and diagnosed using abdominal CT was found to be 37% [19]. US is not sufficiently sensitive to detect HS and the rate obtained may be an underestimation or overestimation of the actual value. CAP may be simultaneously measured with LSM and more precisely 5 Hepatic Steatosis 6 estimates the rate of HS than the previously identified CAP cutoff of 232dB/m. HS is the one symptom of metabolic syndrome. Patients in the present study also showed a close association with metabolic syndrome complicated by SHS [17][18][19]. In a univariate analysis, metabolic factors including triglycerides, fasting plasma glucose, HbA1c, BMI and waist circumference were associated with SHS. In a multivariate analysis, the only factor associated with SHS was BMI. Increases in the BMI of HIV-infected patients may have reflected the presence of obesity or metabolic syndrome involving SHS among such patients.
In the present study, the only factor associated with SHS was BMI. Obesity has been identified as one of the most important risk factors for HS in the general population. HS occurs due to the excessive accumulation of triglycerides in hepatocytes [2,11]. Another mechanism responsible for HS is the impaired oxidation of fatty acids due to mitochondrial toxicity associated with Nucleoside analogue Reverse Transcriptase Inhibitors (NRTI) and Efavirenz. But some studies reported that no relationship existed between HS and antiretroviral drugs [5,[20][21][22]. In the present study, while univariate analysis suggested that oral Tenofovir administration for 2 years or longer prevented SHS, multivariate analysis did not show a relationship [4,18,19]. On the other hand, while univariate analysis suggested that lower percentage of patients with a BMI of 25 or greater when treated with oral Lopinavir was observed (the results not shown in Table 2,3), multivariate analysis did not show a relationship. Thus, SHS and BMI were not more frequently among the patients who had been treated with the oral antiretroviral drugs in the present study.
BMI is known to increase in HIV-infected patients following the initiation of ART. Gracia A, et al. reported that the amounts of limb and visceral fat were higher in HIV-infected patients within the first 96 weeks of ART. As HS is a type of visceral fat, it may be appropriate to consider the redistribution of fat related to HIV infection which increases its volume [23].
In the present study, 28 (46%) patients tested positive for HBsAg and 14 (23%) were positive for HCV Ab. Although 42 (70%) patients had a hepatitis virus co-infection, HBsAg-positive rates, the duration for which patients were positive for HB-sAg, HCV Ab-positive rates and the duration for which patients were positive for HCV RNA were not associated with SHS.
In conclusion, the prevalence of HS was higher as 42.3% among HIV-infected patients. The prevalence of HS needs to be determined more precisely because it progresses to NASH, LC and HCC. Increases in BMI and obesity of HIV-infected patients are the most important factors associated with HS. Clinicians need to recommend changes to diet and lifestyle-related habits in order to lower BMI, thereby reducing the risk of HS progressing to NASH.