Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2016, V. 51, № 4, pp. 467-474.

doi: 10.15389/agrobiology.2016.4.467eng

UDC 636/639:636.4:578:577.083

 

THE INFLUENCE OF TEMPERATURE ON THE RUSSIAN ISOLATE OF
AFRICAN SWINE FEVER VIRUS IN PORK PRODUCTS AND
FEED WITH EXTRAPOLATION TO NATURAL CONDITIONS

I.P. Sindryakova1, Yu.P. Morgunov1, A.Yu. Chichikin1, I.Kh. Gazaev2,
D.A. Kudryashov1, S.Zh. Tsybanov1

1All-Russian Institute of Veterinary Virology and Microbiology, Federal Agency of Scientific Organizations, 1, ul. Akademika Bakuleva, pos. Vol’ginskii, Petushinskii Region, Vladimir Province, 601120 Russia,
e-mail sindryakova.irina@yandex.ru
2Kabardino-Balkarian Reference Center of Rosselkhoznadzor (Federal Service for Veterinary and Phytosanitary Surveillance), 1, ul. 9 Maya, Nalchik, Kabardino-Balkar Republic, 360051 Russia

Received May 23, 2016

 

It is known that one of the ways to spread African swine fever (ASF) virus is alimentary way (e.g., through feeding animals with contaminated feed and slaughterhouse waste products not subjected to thermal treatment, not passed veterinary and sanitary examination, and derived from epidemiologically disadvantaged regions). A variety of preserved food, such as corned beef, canned meat, bacon, etc., is made of pork products and by-products. Persistence of the virus in these products is not fully understood. For the first time we carried out studies on infectious activity of ASF virus isolates circulating in the Russian Federation, in contaminated pig products, feed, as well as in samples intended for veterinary-sanitary supervision. For this purpose, the 3-4 month-old piglets of 20-30 kg body weight were intramuscularly challenged with virus-containing blood. For inoculation we used ASFV isolate Volgograd-Kalach 2012 with the initial infectious titer of 7.5 lg HAU50/cm3 obtained from the National Collection of the SSINRRIVV&M of RAAS. Three days after infection the animals were slaughtered, samples of internal organs and tissues were taken for virus isolation, and the pork was used for making corned beef, canned meat and salted lard. Blood, containing ASF virus, was also used for contamination of a compound feed and drinking water. To study effects of storage temperature on the virus infectious activity and genome detection, the samples were divided into aliquots and stored at 20-25 °С (room temperature), 4-6 °С (a common refrigerator); -16…-20 °С (freezer). The temperatures selected for the storage of samples conform to the natural weather conditions at various seasons (20-25 °С in the summer, -16…-20 °С in winter); 4-6 °С is generally maintained in storerooms or stern room in the winter months. Samples from the aliquots were taken with a 5 day intervals to determine the ASF virus titer and its genome fragments. Virus isolation was carried out in a primary culture of swine bone marrow cells for 5 days in two consecutive passages. Hemadsorption test (HAD) and real-time PCR were conducted for ASFV titer estimation and genome detection. As a result, the ASF virus was found in corned beef and lard stored at 20-25 °С for 16 days. At storage temperature of 4-6 °С the ASF virus was revealed in corned beef during the observation period (60 days). In frozen samples the ASFV infectious activity and DNA fragments were found throughout the entire study period (60 days). Thus, it is found that in food products and by-products from pigs slaughtered soon after ASF infecting the ASFV infectivity persists for a long time depending on the storage conditions and can pose a serious danger as a pathogen transmission factor. The data also allow to predict the temperature conditions conducive to maintaining circulation of the virus in АSF outbreaks.

Keywords: African swine fever, ASF, virus, hemadsorption test (HAD), real-time polymerase chain reaction (qPCR), epizooty, liquidation of infection focus.

 

Full article (Rus)

Full text (Eng)

 

REFERENCES

  1. Mur L., Martinez-Lopez B., Sanchez-Vizcaino J.M. Risk of African swine fever introduction into the European Union through transport-associated routes: returning trucks and waste from international ships and planes. BMC Vet. Res., 2012, 8: 149 CrossRef
  2. Beltran-Alcrudo D., Lubroth J., Depner K., De La Rocque S. African swine fever in the Caucasus. FAO Empres Watch, 2008, April: 1-8.
  3. Dankvert S.A. Epizooticheskaya situatsiya v Rossiiskoi Federatsii po afrikanskoi chume svinei (po sostoyaniyu na 1 oktyabrya 2013 goda) [African swine fever in the Russian Federation — epizootic situation (dated October 01, 2013)] (in Russ.). Available  http://www.fsvps.ru/fsvps/iac/report.html. No date.
  4. Makarov V.V. Afrikanskaya chuma svinei [African swine fever]. Moscow, 2011 (in Russ.).
  5. Gogin A., Gerasimov V., Malogolovkin A., Kolbasov D. African swine fever in the North Caucasus region and the Russian Federation in years 2007-2012. VirusRes., 2013, 173: 198-203 CrossRef 
  6. Vlasov N.A. O razvitii epizooticheskoi situatsii po ACHS na territorii Rossiiskoi Federatsii [On ASF epizootic situation in the Russian Federation] (in Russ.). Available http://www.fsvps.ru/fsvps-docs/ru/iac/2009/files/iac_asf.pdf. No date.
  7. O realizatsii meropriyatii po likvidatsii ACHS na territorii Rossiiskoi Federatsii [On the ASF eradication in the Russian Federation] (in Russ.). Available http://www.fsvps.ru/fsvps-docs/ru/iac/2011/files/asf_2011-06-29.pdf. No date.
  8. Morilla A. Las enfermedades virales emergentes de los cerdos. Cienciaveterinaria, 2003, 9: 197-219.
  9. Wooldridge M., Hartnett E., Cox A., Seaman M. Quantitative risk assessment case study: smuggled meats as disease vectors. Rev. Sci. Tech., 2006, 25: 105-117 CrossRef
  10. Mur L., Martinez-Lopez B., Martinez-Aviles M., Costard S., Wieland B., Pfeiffer D.U., Sanchez-Vizcaino J.M. Quantitative risk assessment for the introduction of African swine fever virus into the European Union by legal import of live pigs. Transbound. Emerg. Dis., 2012, 59: 134-144 CrossRef
  11. Carrascosa A.L., Bustos M.J., de Leon P. Methods for growing and titrating African swine fever virus: field and laboratory samples. In: Current Protocols in Cell Biology. Wiley, Hoboken, 2011. Suppl. 53. Unit 26.14: 26.14.1-26.14.25 CrossRef
  12. Instruktsiya po primeneniyu «Test-sistemy dlya vyyavleniya DNK virusa ACHS metodom PTSR v real'nom vremeni» [qPCR kit for indication ASFV DNA: instruction]. Pokrov, 2013 (in Russ.).
  13. Morgunov Yu.P., Petrov Yu.I. Problemy biologii produktivnykh zhivotnykh, 2010, 4: 104-111 (in Russ.).    
  14. EFSA AHAW Panel (EFSA Panel on Animal Health and Welfare). EFSA Journal, 2015, 13(7): 4163 CrossRef
  15. Spickler A.R., Roth J.A. African swine fever. In: African swine fever. Iowa State University, College of Veterinary Medicine, 2011.
  16. Bronsvoort B.M.D., Alban L., Greiner M. Quantitative assessment of the likelihood of the introduction of classical swine fever virus into the Danish swine population. Prev. Vet. Med., 2008, 85: 226-240 CrossRef
  17. de Vos C.J., Saatkamp H.W., Nielen M., Huirne R.B. Scenario tree modeling to analyze the probability of classical swine fever virus introduction into member states of the European Union. Risk Analysis, 2004, 24: 237-253 CrossRef
  18. Adkin A., England T., Hall S., Coburn H., Marooney C.J., Seaman M., Cooper J., Hartnett E. Estimating the risk of exposure of British livestock to foot-and-mouth disease associated with the importation of ship and aircraft waste. Vet. Rec., 2008, 163: 235-240 CrossRef
  19. Corso B. Likelihood of introducing selected exotic diseases to domestic swine in the continental United States of America through uncooked swill. Rev. Sci. Tech., 1997, 16: 199-206 CrossRef
  20. Gale P. Risks to farm animals from pathogens in composted catering waste containing meat. Vet. Rec., 2004, 155: 77-82 CrossRef
  21. Iglesias I., Muñoz M.J., Montes F., Pérez A., Gogin A., Kolbasov D., de la Torre A. Reproductive ratio for the local spread of African swine fever in wild boars in the Russian Federation. Transbound. Emerg. Dis., 19 FEB 2015 CrossRef
  22. Costard S., Jones B.A., Martínez-López B., Mur L., de la Torre A., Martínez M., Sánchez-Vizcaíno F., Sánchez-Vizcaíno J.M., Pfeiffer D.U., Wieland B. Introduction of African swine fever into the European Union through illegal importation of pork and pork products. PLoS ONE, 2013, 8(4): e61104 CrossRef 
  23. Adkin A., Coburn H., England T., Hall S., Hartnett E., Marooney C., Wooldridge M., Watson E., Cooper J., Cox T., Seaman M. Risk assessment for the illegal import of contaminated meat and meat products into Great Britain and the subsequent exposure of GB livestock (IIRA): foot and mouth disease (FMD), classical swine fever (CSF), African swine fever (ASF), swine vesicular disease (SVD). New Haw, Veterinary Laboratories Agency, 2004.
  24. Sánchez-Vizcaíno J.M., Mur L., Martínez-López B. African swine fever (ASF): five years around Europe. Vet. Microbiol., 2013, 165(1-2): 45-50 CrossRef
  25. Scientific Opinion on African swine fever. EFSA Panel on Animal Health and Welfare (AHAW). EFSA Journal, 2010, 8(3): 1556 CrossRef
  26. Mebus C., Arias M., Pineda J., Tapiador J., House J., Sánchez-Vizcaíno J.M. Survival of several porcine viruses in different Spanish dry-cured meat products. Food Chem., 1997, 59(4): 555-559 CrossRef

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