Experimental Animals
Online ISSN : 1881-7122
Print ISSN : 0007-5124
Comparison of the Intestinal Bacteria in Specific Pathogen Free Mice from Different Breeders
Kazuhiro HIRAYAMAKimiko ENDOSeiji KAWAMURATomotari MITSUOKA
Author information
JOURNAL FREE ACCESS

1990 Volume 39 Issue 2 Pages 263-267

Details
Abstract

Specific pathogen free BALB/c mice from 3 commercial laboratory animal breeders in Japan were compared on the composition of caecal flora revealed by selective and nonselective cultivation as well as direct microscopical observation on smears, and relative caecal weight. Large differences were detected in viable counts of total bacteria and almost all bacterial groups, while direct microscopical counts which consisted mainly of fusifrom bacteria were almost equal, resulting in diverse recovery rates among 3 breeders.Eubacteriumand spiral shaped organisms were not detected from all breeders. There also existed variations within breeders, especially those in the number of Enterobacteriaceae. Relative caecal weight also differed among breeders, suggesting the participation of variety of caecal bacteria in determining this parameter. As these variations in bacteriological status of animals can influence experimental results. standardization of intestinal microbial flora is required.

Content from these authors
© Japanese Association for Laboratory Animal Science
Previous article Next article
feedback
Top