Many experimental techniques for bile collection have been investigated. Most have been used under conditions of anaesthesia, or early after biliary cannulation. These conditions affect both the volume and the components of the bile, and the liver metabolism. In order to solve these problems, we devised an experimental method in which a T-cannula was introduced into the common bile duct of beagles that had been trained to remain at rest on the experimental table. Using this method, all of the bile could be collected under conscious conditions without the influence of surgical invasion.