Sdt97: A Point Mutation in the 5′ Untranslated Region Confers Semidwarfism in Rice

Semidwarfism is an important agronomic trait in rice breeding programs. The semidwarf mutant gene Sdt97 was previously described. However, the molecular mechanism underlying the mutant is yet to be elucidated. In this study, we identified the mutant gene by a map-based cloning method. Using a residual heterozygous line (RHL) population, Sdt97 was mapped to the long arm of chromosome 6 in the interval of nearly 60 kb between STS marker N6 and SNP marker N16 within the PAC clone P0453H04. Sequencing of the candidate genes in the target region revealed that a base transversion from G to C occurred in the 5′ untranslated region of Sdt97. qRT-PCR results confirmed that the transversion induced an obvious change in the expression pattern of Sdt97 at different growth and developmental stages. Plants transgenic for Sdt97 resulted in the restoration of semidwarfism of the mutant phenotype, or displayed a greater dwarf phenotype than the mutant. Our results indicate that a point mutation in the 5′ untranslated region of Sdt97 confers semidwarfism in rice. Functional analysis of Sdt97 will open a new field of study for rice semidwarfism, and also expand our knowledge of the molecular mechanism of semidwarfism in rice.

ABSTRACT Semidwarfism is an important agronomic trait in rice breeding programs. The semidwarf mutant gene Sdt97 was previously described. However, the molecular mechanism underlying the mutant is yet to be elucidated. In this study, we identified the mutant gene by a map-based cloning method. Using a residual heterozygous line (RHL) population, Sdt97 was mapped to the long arm of chromosome 6 in the interval of nearly 60 kb between STS marker N6 and SNP marker N16 within the PAC clone P0453H04. Sequencing of the candidate genes in the target region revealed that a base transversion from G to C occurred in the 59 untranslated region of Sdt97. qRT-PCR results confirmed that the transversion induced an obvious change in the expression pattern of Sdt97 at different growth and developmental stages. Plants transgenic for Sdt97 resulted in the restoration of semidwarfism of the mutant phenotype, or displayed a greater dwarf phenotype than the mutant. Our results indicate that a point mutation in the 59 untranslated region of Sdt97 confers semidwarfism in rice. Functional analysis of Sdt97 will open a new field of study for rice semidwarfism, and also expand our knowledge of the molecular mechanism of semidwarfism in rice. Dwarfism is one of the most important agronomic traits of rice. The introduction of high-yielding dwarf cultivars, together with the application of large amounts of fertilizer and pesticides-termed the 'Green Revolution'-has contributed substantially to the significant increase in rice production, and largely averted the chronic food shortage that was feared after the rapid expansion of the world population in the 1960s (Khush 2001;Sasaki et al. 2002;Hedden 2003;Muangprom and Osborn 2004).
Utilization of dwarf rice cultivars was one of the greatest achievements in rice breeding in the 20th century. Before the introduction of the dwarf gene to rice improvement programs, rice cultivars were all tall; the stems of tall rice plants were not strong enough to support the heavy grain of the high-yielding varieties, and this resulted in plants falling over. This process, known as lodging, resulted in large yield losses (Hedden 2003).
Semidwarf plants possess short, strong stalks, exhibit less lodging, and a greater proportion of assimilation partitioned into the grain, resulting in further yield increases (Hedden 2003). Reducing plant height to breed high-yielding, nonlodging, rice varieties has become a predominant strategy for breeders since the 1960s (Hargrove and Cabanilla 1979). Dwarf genes have been utilized extensively in plant breeding to improve lodging resistance (Khush 2001;Sasaki et al. 2002).
The dwarfing gene originated from a Chinese cultivar, Dee-geo-woogen, which was used in a breeding program in Taiwan during the 1950s to produce the highly successful Taichung Native 1. This cultivar was also used later at the International Rice Research Institute (IRRI) in the Philippines to produce IR-8. Semidwarf, high-yielding cultivars have also been produced independently in the People's Republic of China, Japan and the USA. The semidwarf trait can be attributed to the different alleles of a single recessive gene, sd1, even where the parent strains have been selected or produced independently (Hedden 2003;Sasaki et al. 2002;Kikuchi and Futsuhara 1997).
In previous research, we isolated a dominant semidwarf mutant gene Sdt97 (Tong et al. 2007). In this article, we report current progress in the molecular cloning of Sdt97.
(RHL). Y98149 was isolated originally from an F 6 generation nursery selection of a medium japonica rice cross between M9056 and R8018 Xuan. Y98149 and Y98148 are near isogenic lines (NIL) (Tong et al. 2003).
Field trials were carried out on paddy fields in two locations. (1) During the natural rice growing seasons in Hefei in 2006, Y98149, Y98148, Huajingxian74, and F 7 RHL were planted at the Experimental Station at Hefei (31 · N, 117 · E), Anhui province, China. Seeds were sown in seed beds on April 15-20, 2006. On June 15-20, 1-month old seedlings were transferred to the paddy field for further growth. (2) During the natural early rice growing seasons in Sanya in 2006-2007, Y98149, Y98148, Hua jingxian74, andF 7:8 RHL were planted in the field at experimental stations at Sanya (18 · N, 109 · E), Hainan province, China. Seeds were sown in seed beds on November 15-20, 2006. On December 15-20, 2006, 1-month old seedlings were transferred to the paddy field for further growth.
The planting density was 13.3 cm between plants in a row, and the rows were 16.7 cm apart. Field management, including irrigation, fertilizer application, and pest control, followed normal agricultural practice.

Phenotypical characterization and genetic analysis
In this research, plant heights were recorded at maturity in the paddy field. Plant height was measured from ground level to the plant material tassel tip. Statistical significance was assessed using Student's t-test. Probability values (P) 0.05 were considered significant. Segregation of plant height phenotype in RHL segregated progenies was analyzed for goodness of fit in the ratio of 3:1 using a Chi-square test (x 2 , x 2 0.05 , 1 = 3.84). Each quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed for three biological replicates. The DDCt method was used for data analysis.

Mapping population development
An intersubspecific cross between semidwarf mutant Y98149 (japonica cv.) and Hua-jing-xian74 (indica cv.) was developed for the Sdt97 mapping. A F 6 recombinant inbred line population (RIL) was identified to be a RHL, and was used in the rough mapping of Sdt97 (Tuinstra et al. 1997;Yamanaka et al. 2005;Tong et al. 2007).
In the fine mapping study of Sdt97, a large scale F 7 RIL population was constructed. Out of this segregating F 7 RHL population, F 7 recessive tall individuals were selected for the fine mapping of Sdt97. For the genetic validation, or progeny testing of the recessive F 7 individuals, F 7:8 RHL derived from these tall F 7 recessive individuals were further planted in the field at experiment stations in Sanya (18 · N, 109 · E), Hainan province, China. The developmental process of the RHL population was as shown in Figure 1.

DNA extraction and molecular marker development
Fresh leaves were collected and ground in liquid nitrogen. DNA was extracted from the ground tissues using the modified CTAB (celytrimethyl ammonium bromide) method (Rogers and Bendich 1998).
Single nucleotide polymorphisms (SNPs) and cleaved amplified polymorphic sequence (CAPS) markers were developed by sequencing the PCR products amplified from japonica cv. semidwarf mutant Y98149, and Indica cv. Huajingxian74 in the rough mapping region of Sdt97. PCR products were cloned into the vector pGEM-T (Promega, USA) for sequencing, and for developing SNPs. PCR amplifications followed the profile: 94°for 3 min, 35 cycles of 94°for 1 min, 55°f or 1 min and 72°for 1 min, with a final extension of 72°for 5 min. The markers, which showed polymorphism between Y98149 and Huajingxian74, were then utilized for Sdt97 fine mapping. The LightCycler480 Real-Time PCR System was employed for SNP Genotyping. PCR amplification was performed in a 96-well plate in the LightCycler 480 Real-Time PCR System. Reaction volume was 10 ml; 2 ml of genomic DNA (10 ng/ml) was added to 8 ml of the reaction master mixture consisting of 1· LightCycler 480 High Resolution Melting Master (containing the proprietary ds-DNA saturating binding dye), with 2.5 mM MgCl 2 (Roche Diagnostics, Germany), and 0.5 mM of forward and reverse primers. The PCR program started with an initial denaturation of 10 min at 95°, and continued with 40 cycles of 10 sec at 95°, 15 sec at 60°, and 10 sec at 72°. For high resolution melting (HRM), the plate was heated from 65°to 95°, performing 25 acquisitions per 1° (Norambuena et al. 2009).

Map-based cloning
Using the RHL, Sdt97 was mapped to a 118-kb region on chromosome 6 flanked by two STS markers, N6 and TX5 (Tong et al. 2003). Bulked segregant analysis (BSA) (Michelmore et al. 1991) combined with recessive class analysis (RCA) (Chen et al. 2005;Pan et al. 2003;Zhu et al. 2004;Zhang et al. 1994) were used to identify molecular markers linked to the mutant gene Sdt97 in the fine mapping of Sdt97. Genomic DNA from 30 tall individuals and 30 dwarf individuals in the F 6:7 segregated progenies were pooled to create the tall and dwarf DNA bulks, respectively. The parental DNA, and the two bulks, were used for BSA. Polymorphic markers from the two parents were screened against the two DNA bulks, and polymorphic markers between the two DNA bulks were screened against the entire population of tall recessive individuals in the F 6:7 segregated population.
Using the markers developed in this research, the mutant gene Sdt97 was finally mapped, the candidate genes detected in the target region were PCR-amplified and sequenced for mutation detection. The average length of the PCR products was 1.5 kb, and the PCR products were sequenced directly.

RNA manipulation and qRT-PCR analysis
To analyze the expression pattern of Sdt97 carried by the semidwarf mutant Y98149, sdt97 carried by tall wild-type Y98148, and the gene expression of Sdt97 in transgenic lines, samples were prepared from Y98149, Y98148, and the transgenic T 1 lines of F 4 plants at seedling stage, tillering stage, elongation stage, and milk ripe stage, respectively.
Fresh plant tissues of above-ground rice were harvested, and immediately ground to fine powder in liquid nitrogen. Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech) were used to synthesize cDNA using an anchored oligo(dT) 18 primer according to the kit instructions.
qRT-PCR (20 ml reaction volume) was carried out using 0.5 ml cDNA, 0.2 ml of each gene-specific primer, and SYBR Premix Ex Taq Kit (TaKaRa) in a LightCycler Real Time PCR according to the manufacturer's instructions. Reaction conditions were 95°for 30 sec, followed by 40 cycles of 95°for 5 sec, and 60°for 31 sec. The standard procedure for melt curve analysis followed the amplification cycles. The rice actin gene was used as the endogenous control for normalization. Each real-time PCR analysis was performed for three biological replicates. The relative quantification method (DDCt) was used for data analysis.

Vector constructions and plant transformation
To confirm further that the mutated traits were caused by mutant gene Sdt97, we constructed transgenic plants for the complement test of Sdt97. The Gateway cloning system was adopted, and the Gateway vector pGWB12:[(35S promoter,N-FLAG) (-35S promoter-FLAG-R1-CmR-ccdB-R2-)] (Invitrogen) was used for the Sdt97 complementarity test. We wanted to test the effect of the point mutation in the 59 untranslated region (59-UTR) on the gene expression of Sdt97 itself.
Considering that the point mutation in the 59-UTR may be associated with the promoter of Sdt97, perhaps the point mutation in the 59-UTR is part of the promoter itself, thus pGWB3: [(no promoter, C-GUS) (-R1-CmR-ccdB-R2-GUS -)] (Invitrogen nomenclature), a no promoter Gateway vector, was also used in the complementary test of Sdt97.
Two Sdt97 genomic DNA fragments were amplified by PCR from the genomic DNA using the following primers: BF 1 : GTGGGGACAA GTTTGTACAAAAAAGCA GGCTTCCCTCTCCTCTCCTCTCACCA CC, BR 1 : GTGGGGACCACTTTGTACAAGAAAGCTGGGTCTGTTAA CTGATTGAGGAAGATTTTTCA, BF 2 : GTGGGGACAAGTTTGTAC AAAAAAGCAGGCTTCGTGAGATAATGCCGGGCCC, and BR 2 : GTGGGGACCACTTTGTACAAGAAAGCTGGGTCAGGTATCCTG CAGTTCTGCATG. A 2701-bp genomic DNA fragment containing the entire Sdt97, and a 3116-bp genomic DNA fragment containing the entire Sdt97, the 174 bp 59 upstream sequence and the 243 bp 39 downstream sequence, were amplified with high fidelity using KOD-Plus-Neo (TOYOBO, Japan). The PCR amplification products were mixed with Donor vectors (Invitrogen nomenclature) and BP Clonase enzyme mix to construct an Entry clone. Then, the Entry clone was transferred into the Gateway vector using the enzyme mix, and LR Clonase to construct the Expression clone. The expression clone was constructed from the 3116-bp PCR product and pGWB12, and was designated as F 4 . The expression clones constructed from the 2701 bp and 3116 bp PCR products and pGWB3 were named T 6 andT 15 , respectively. The expression clone was introduced into the Agrobacterium strain EHA105 by electroporation, and transformation was carried out as described (Hiei et al. 1994;Xue et al. 2008).
Positive transgenic plants were detected by sequencing the PCR products of the genomic DNA using a pair of primers (forward primer: CCTCTCCTCTCCTCTCACCACC; reverse primer:TAATCCCAGCC CAGGGTTCG) designed according to the sequence mutation at the promoter region. The PCR products were 223 bp, and it was easy to separate the transgenic plants from that of transgenic acceptors line or tall wild type plants. Transgenic lines were selected using hygromycin treatment (15 mg L-1).The transgenic rice plants were transferred to and grown in experimental fields from January to April in Sanya, Hainan Province, and from May to October in Hefei Anhui Province China 2014.

Data availability
The authors state that all data necessary for confirming the conclusions presented in the article are represented fully within the article.

RESULTS
Isolation and phenotypic characterization of the semidwarf mutant In 1997, a semidwarf mutant was isolated from the tall F 6 progenies derived from the cross between M9056 and R8018 XUAN (Tong et al. 2001). The semidwarf mutant was deduced to result from a spontaneous  mutation, and not from the cross-fertilization between the tall wild type and other dwarf rice cultivars (Tong et al. 2003). It is the NIL to the tall wild type in the same selected nursery, the semidwarf mutant was named Y98149, and the tall wild type was named Y98148 (Tong et al. 2003).
The length of each internode of the mutant is almost uniformly shortened, resulting in an elongation pattern similar to that of the wildtype plant (Table 1). According to the grouping of rice dwarf cultivars proposed by Takahashi, the semidwarf mutant belongs to the dn-type (Takahashi et al.and Takeda 1969).
Research into the genetic character of the semidwarf mutant revealed that there was only one dominant gene locus involved in the control of the semidwarfism, and the semidwarfism expression of the mutant was not affected by its cytoplasm (Figure 2). The results of classic genetic allelic tests revealed that the semidwarf mutant gene is a new kind of semidwarf gene reported in rice; the mutant gene was temporarily designated Sdt97 (Tong et al. 2003(Tong et al. , et al. 2007).

RHL population development
For the sdt97 mapping, an intersubspecific cross between semidwarf mutant Y98149 (japonica cv.) and Hua-jing-xian74 (indica cv.) was developed. A F 6 recombinant inbred line population (RIL), which originated from the intersubspecific cross, was identified as an RHL (Yamanaka et al. 2005;Tuinstra et al. 1997;Tong et al. 2007). The heterozygous chromosomal region is approximately 25.5 cM and 6646 kb, starting from RM3430 and ending at RM6395. This was initiated by different parents, one carrying the Sdt97 gene derived from the mutant, and the other carrying the sdt97 gene derived from Hua-jing-xian74 (Table 2). This F 6 residual heterozygous lines population was used in the rough mapping of Sdt97, and the semidwarf mutant gene Sdt97 was mapped on the long arm of chromosome 6 at the interval between two STS markers N6 and TX5, with a genetic distance of 0.2 cM and 0.8 cM, respectively (Tong et al. 2007).
A contig map was constructed based on the reference sequence aligned by the Sdt97 linked markers, and the mutant Sdt97 locus was defined to a 118-kb interval within the PAC clone P0453H04 (Sequence ID:dbj| AP005453.1| length: 175,047 bp) (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
In the F 6:7 progenies, 44 populations derived from homozygous dwarf F 6 individuals, exhibited dwarf nonsegregation; 82 populations derived from heterozygote dwarf F 6 individuals showed continued plant height segregation, and resulted in both tall and dwarf individuals (Figure 3). The ratio of the segregating F 6:7 progenies to the nonsegregating dwarf F 6:7 progenies is 1.86, which fits the expected Mendelian segregated ratio of 2:1 (x 2 = 0.081, P . 0.05). The results confirmed that there was only one dominant gene controlling the segregation of plant height in the RHL population.
A large scale F 7 RIL population, consisting of 10,000 F 7 individuals, was constructed. Of these segregating F 7 RHL plants, 2328 F 7 tall recessive individuals were selected for the fine mapping of Sdt97 using the RCA method (Figure 4, Tuinstra et al. 1997;Yamanaka et al. 2005;Tong et al. 2007). For genetic validation or progeny testing of the recessive F 7 individuals, 2328 F 7:8 RHL derived from these tall F 7 recessive individuals were further planted in the field at the experimental stations in Sanya (18 · N, 109 · E), Hainan province, China. A total of 2312 of them, which passed progeny testing, was further used for the fine mapping of Sdt97 in this study.

Fine mapping of Sdt97
Upon further linkage analysis, one cleaved amplified polymorphic sequence (dCAPS) (BamHI), and 16 SNP markers were developed by Figure 5 PCR products were amplified with SNP marker N16 from tall recessive individuals in the segregating F 6:7 populations, and analyzed by high-resolution-melting (HRM). Three types of HRM curves were obtained: curve A represents homozygous Y98149, curve B represents homozygous Hua-jing-xian74, and curve C represents heterozygous RHL. sequencing the PCR products amplified from Y98149 and Huajing-xian74 in the target region (Supplemental Material, File S1). Depending on sequence variation, different SNP genotype of the recessive individuals in the F 6:7 populations be differentiated ( Figure 5).
A total of 2312 tall recessive individuals in the 86 F 6:7 segregated population was used. By analyzing BSA and RCA, 33 distinct recombinants were identified in the target region. Using one dCAPS marker (BamHI), and 16 new SNP markers developed in this research, the Sdt97 gene was further mapped to the long arm of chromosome 6 at the interval of nearly 60 kb between STS markers N6 and SNP marker N16 within the PAC clone P0453H04 (Sequence ID: dbj|AP005453.1 | length: 175,047 bp). The sequence of primers N6 and N16, and their location on the PAC clone P0453H04, are as shown in Table 3 (see http://blast.ncbi.nlm.nih.gov/Blast.cgi).

Map-based cloning of the Sdt97 gene
Based on the available sequence annotation database (http://www. gramene.org/;http://rice.plantbiology.msu.edu/index.shtml), nine genes were predicted in the 60 kb target region of the cultivated rice genome. To identify the candidate gene of Sdt97, the genomic DNA sequence of all nine predicted genes in the 60 kb target region were sequenced. Based on the sequence analysis, nine genes could be categorized under three headings (Table 4).
There is no DNA sequence difference between the semidwarf mutant and the tall wild type; LOC_Os06g44040, LOC_Os06g44060, LOC_Os06g44070, LOC_Os06g44080, LOC_Os06g44090, and LOC_Os06g440100 were of this type.
Multicopy genes exist in the rice genome; LOC_Os06g44120 and LOC_Os06g44130 were of this type. Two copies of LOC_Os06g44120 [LOC_Os06g44120; LOC_Os12g34630], and three copies of LOC_ Os06g44130 [LOC_Os06g44130; LOC_Os03g19150; LOC_ Os10 g08300], are known to exist in the rice genome.
Sequence analysis of genomic DNA fragments of the gene from tall wild-type and the semidwarf mutant showed that a point mutation occurred in the 59-UTR of LOC_Os06g44050, resulting in a transversion from G to C.
The transversion took place at a site 277 bp upstream of the initiation codon in the 59-UTR of exon 1 (Figure 6). The mutant base of Sdt97 carried by Y98149 was C, while sdt97 alleles of the same gene occupying the equivalent locus on homologous chromosomes carried by Y98148 had a G in this position (Figure 7). Compared with the genomic DNA sequence of Nipponbare, it was found that not only Y98149, but also Y98148, had a 342-bp deletion in the first coding exon in LOC_ Os06g44 050. The deletion occurred between 546 bp and 887 bp in the genomic sequence, or between 194 bp and 535 bp in the coding sequence (CDS) of LOC_Os06g44050 (Figure 8). Thus, LOC_Os06g44050 was finally identified as the candidate gene of Sdt97.
The genomic DNA and cDNA nucleotide sequences of Sdt97 were 2701 bp and 1517 bp in length, respectively. A schematic gene model of Sdt97 is displayed in Figure 6. The DNA and cDNA sequences of Sdt97 and the deletion fragment are provided.
Transversion from G to C in the 59-UTR of Sdt97 alters the expression pattern At different stages of growth and development of Y98149 and Y98148, qRT-PCR was applied to analyze the effect of the transversion from G to C in the 59-UTR of Sdt97 on its own gene expression. The results of qRT-PCR were as follows (Table 5): n Table 4 The information and sequence alignment of the genes in Y98148, Y98149 in the 60 kb target region  (3) Note that the 342-bp deletion fragment in LOC_Os06g44050 is observed between Y98149 (or Y98148) and Nipponbare.
n At the seedling stage, the DDCt = 0.12, the N relM /N relWT = 2 -DDCt = 0.92, meaning that the gene expression of Sdt97 in Y98149 was just the same level as that in Y98148.
At the tillering stage, the DDCt = -2.12, the N relM /N relWT = 2 -DDCt = 4.37, meaning that the gene expression of Sdt97 in Y98149 was four times higher than that of sdt97 in Y98148.
In contrast, at the elongation stage, the DDCt = 2.06, the N relM /N relWT = 2 -DDCt = 0.24, meaning that the gene expression of sdt97 in Y98148 was four times higher than that of Sdt97 in Y98149. At the milk ripe stage, the DDCt = 0, the N relM /N relWT = 2 -DDCt = 1.00, meaning that the gene expression of sdt97 in Y98148 was just the same as, or just more than, Sdt97 in Y98149.
The expression level of the gene Sdt97 in the transgenic lines is very strong supporting evidence for the function of sdt97. In this study, qRT-PCR was applied at different growth stages to analyze the effect of the gene transfer of Sdt97 on the expression pattern of this gene in transgenic T 1 lines of F 4 . Table 6 provides qRT-PCR results.
At the seedling stage, the expression level of the gene Sdt97 in transgenic T 1 lines of F 4 was just the same as that in the transgenic receptor, the tall wild type, Y98148. At the tillering stage, the expression level of the gene Sdt97 in the transgenic T 1 lines of F 4 increased, DDCt = -0.66, the N relF4 /N relWT = 2 -DDCt = 1.58, i.e., 1.58 times higher than that in the transgenic receptor, Y98148. At the elongation stage, in contrast, the expression level of the gene Sdt97 in transgenic T 1 lines of F 4 decreased, DDCt = 0.22, the N relF4 /N relWT = 2 -DDCt = 0.86, i.e., lower than that in the transgenic receptor, Y98148. At the milk ripe stage, the expression level of the gene Sdt97 in transgenic T 1 lines of F 4 was just the same as, or just slightly higher than that in the tall wild type Y98148.
The results showed that gene transfer of Sdt97 induced obvious changes in the expression of this gene in transgenic T 1 lines of F 4 , with a pattern similar to that in the semidwarfism mutant Y98149.
These results indicated that the G-C transversion that occurred in the 59-UTR of Sdt97 induced obvious changes in the expression pattern of the Sdt97 gene carried in Y98149 and the transgenic lines, it altered the expression pattern of the gene Sdt97 itself, and led to an obvious change in plant height performance of Y98149 and the transgenic lines.
A transgenic Sdt97 line driven by a no-promoter version of the gateway vector (pGWB3) to tall wild type displays the semidwarf mutant phenotype To highlight the effect of the point mutation in the 59-UTR on the gene expression of Sdt97 itself, a no-promoter Gateway vector,  pGWB3 [(no promoter, C-GUS)(-R1-CmR-ccdB-R2-GUS-)] (Invitrogen), was used to carry out the complementarity test of Sdt97 in this study.
The genotypes of the transgenic plants were determined by sequencing PCR products. The transgenic acceptor lines with overlapping peaks of C and G at the nucleotide location of 124 bp in the DNA sequencing chromatogram can be easily and clearly detected ( Figure 9).
Forty-one independent transgenic T 0 lines for T 6 , and 25 independent transgenic T 0 lines for T 15 were obtained. Most of the independent T 0 , T 1 transgenic lines exhibited a semidwarf phenotype like that of the mutant (Y98149). Genetically, the positive transgenic T 0 , T 1 plants were the same as that of the (Y98148/Y98149)F 1 ,F 2 progeny derived from crosses between Y98148 and Y98149. Because only pGWB3, which has no 35S promoter, was used in the transgenic clone construction of Sdt97, the CDS sequence of Sdt97 in Y98149 was coincident with that of sdt97 in Y98148. Therefore, the point mutation in the 59-UTR region of Sdt97 was responsible for restoration of the semidwarfism mutant phenotype in the transgenic lines ( Figure 10).
A Sdt97 transgenic line driven by the 35S promoter (tall wild type) displays a more pronounced dwarf phenotype than the mutant In a complementarity test of Sdt97, we constructed transgenic plants. The mutant gene Sdt97 driven by the 35S promoter (F 4 ) was introduced into tall wild-type plants (Y98148) via Agrobacterium-mediated transformation. To confirm further that the mutated trait of semidwarfism was caused by a point mutation in the 59-UTR of Sdt97, 14 independent transgenic T 1 lines of the F 4 generation were obtained. Most of the independent T 1 transgenic lines exhibited a more pronounced dwarf phenotype than the semidwarf mutant (Y98149) (Figure 11). The results indicated that Sdt97 overexpression in the genetic background of the tall wild type (Y98148) caused a more pronounced dwarf phenotype than the semidwarf mutant (Y98149).
D2 encodes a novel cytochrome P450 classified as CYP90D, The D2/CYP90D2 is an enzyme catalyzing a step in the late BR biosynthesis pathway (Hong et al. 2003). The brd2 gene is defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1 (Hong et al. 2003). D11 encodes a novel cytochrome P450 (CYP724B1), D11/CYP724B1 is involved in the BR biosynthesis network in rice (Tanabe et al. 2005).
Osdwarf4-1 has a weak dwarf phenotype of OsDWARF4 . Bai et al. (2007) identified OsBZR1-interacting proteins. OsBRI1, d61-7, DLT, and three MADS box proteins are defective in response to BRs in rice (Yamamuro et al. 2000;Morinaka et al. 2006;Tong et al. 2009;Duan et al. 2006;Lee et al. 2008). The strigolactones are rhizosphere-signaling molecules as well as a new class of plant hormones with an increasing number of biological functions (Ruyter-Spira et al. 2013). The gsor23 encodes a key enzyme involved in the biosynthesis of strigolactones (SIs) (Wang et al. 2013). D53 encodes a substrate of the SCFD3 ubiquitination complex and functions as a repressor of SL signaling (Jiang et al. 2013;Zhou et al. 2013). In 2012, a gain-of-function epi-allele (Epi-df) of rice fertilization-independent endosperm1 (FIE1) was identified by Zhang et al. (2012). Epi-df is the only dwarf mutant gene entirely unrelated to plant hormones reported in rice. It has no altered nucleotide sequence but is hypomethylated in the 59 region of FIE1, resulting in a dwarf stature (Zhang et al. 2012).
Sdt97 was deduced to have resulted from a spontaneous mutation. In our previous research, Sdt97 was mapped to the long arm of chromosome 6 at the interval between two STS markers, N6 and TX5, at a genetic distance of 0.2 cM and 0.8 cM, respectively. In a recent study, Sdt97 was fine-mapped to a interval of nearly 60 kb within the PAC clone P0453H04, and LOC_Os06g44050 was identified as the candidate gene of Sdt97. Sdt97 is not allelic to the sd-1 gene on chromosome 1 (Monna et al. 2002;Sasaki et al. 2002;Spielmeyer et al. 2002), sd-t, sd-t2 on chromosome 4 (Li et al. 2001;Jiang et al. 2002 ;Zhao et al. 2005), sd-g, sd-n on chromosome 5 (Liang et al. 1994(Liang et al. , 2004Li et al. 2003), D53 on chromosome 11 (Wei et al. 2006), or the dwarf gene Dx on chromosome 8 (Qin et al. 2008). Clearly, Sdt97 is a novel semidwarf gene reported in rice (Tong et al. 2007).
Until now, no phenotype, disease, or trait is known to be associated directly with LOC_Os06g44050 (the candidate gene of Sdt97), and no phenotype, disease, or trait is associated with these gene variants in plants (www.gramene.org). Methyladenine glycosylase has been n Table 6 The relative quantification of the transgenic T 1 lines of F 4 /the transgenic receptor, the tall wild-type, Y98148 (NrelF 4 /NrelWT) at different growth stages reported only in Mycobacterium bovis BCG (Liu et al. 2014). Therefore, further research into Sdt97 will open new fields of research into rice semidwarfism, with emphasis on the regulation of Sdt97 expression at the transcriptional level, and the relationship between point mutations in the 59-UTR and Sdt97 promoter, the relationship between methyladenine glycosylase and the semidwarf phenotype of the mutant, and the physiological and biochemical pathways of Sdt97 at the molecular level.
Sdt97 could be used as an elite cultivar to breed super-high-yielding hybrid rice in China Dwarf resources are the foundation to realizing the ideal plant type in rice breeding (Zhu 1980). Since the 1960s, the discovery and identification of new dwarf gene resources in rice have gained huge attention. However, these genes are associated with traits such as severe dwarfism, floret sterility, or abnormal plant and grain development; therefore, most of them have not been used in crop improvement (Aquino and Jennings 1966;Kinoshita 1995;Tong et al. 2003). In recent years, new semidwarf genes nonallelic to sd-1 have been identified (Liang et al. 1994(Liang et al. , 2004Li et al. 2001Li et al. , 2003Jiang et al. 2002;Zhao et al. 2005), but sd-1 is still the primary semidwarf gene used in rice breeding (Kinoshita 1995). It is reported that about one-half of the stock from the IRRI collection is allelic to Dgwg (Gu & Zhu 1979). In southern China, 75% of the semidwarf gene found in varieties of economic importance is at the same locus as sd-1 (Min et al. 1996). Frequent usage of the sd-1 gene may reduce genetic diversity and bring about genetic vulnerability to pests and diseases. According to reports, in Asia, the genetic distance of intersubspecies was nearly equal to that of intervariety in rice (Hong 1999), while the genetic distance of the improvement rice varieties, and their parents, was less than half of that of intersubspecies (Zhuang et al. 1997). It is therefore imperative to develop a new source in order to broaden the genetic basis of semidwarfism. The discovery and utilization of a dominant semidwarf gene may be of great significance, not only in genetic theory but also in rice breeding practice (Tong et al. 2001(Tong et al. , 2003. The semidwarf mutant genes reported here are just the ideal gene resources of the dominant semidwarf, and could provide a power genetic tool not only to resolve the problem of excessive height in the intersubspecific F 1 hybrid, but also to breed an ideal type of 'super-highyielding rice'. In our previous research, the semidwarf mutant gene Sdt97 and the photoperiod-sensitive genic male sterile gene pms3 were combined, and a series of semidwarf PSGMS rice was bred successfully, which is now available for a two-line hybrid rice breeding program (Tong et al. 2007).