1. The Carnoy's fluid and 45% acetic acid are good fixatives to fix the nuclear material of yeast cells.
2. The method in which the treatment with cell-wall lytic enzyme, the fixation with Carnoy's fluid, hydrolysis with 1 N-HCl at 60°and staining with aceto-carmine or Giemsa's staining are used subsequently is very effective to observe the nuclear material.
3. The solution of CdCl₂ is effective to fix the spindle of the yeast-cell.
4. Carnoy's fluid, 45% acetic acid and Flemming's solution can fix the contents of the vacuole fairly well.
5. The nucleus changes to the spindle when the mitosis begins and the chromosomes appear in the spindle, overlapping the vacuole.