H₂O₂ 처리된 LLC-PK₁세포에서 Redox Status 및 NF-κB Signaling에 대한 夏枯草의 효과

손종석; 정지천

doi:10.15188/kjopp.2016.08.30.4.242

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한의병리학회
동의생리병리학회지 학술저널
동의생리병리학회지 제30권 제4호
2016.8 242 - 249 (8page)
DOI : 10.15188/kjopp.2016.08.30.4.242

저자정보

손종석 (동국대학교)
정지천 (동국대학교)

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초록·키워드

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This study was to investigate the anti-inflammatory effects of Prunellae Herba(PH). The generation of superoxide anion radical (․O₂-), nitric oxide (NO), peroxynitrite (ONOO-) and Prostaglandin E₂(PGE₂) were measured in the H₂O₂-Treated renal epithelial cells(LLC-PK1 cell) of mouse. And the effects of Prunellae Spica on the expression of NF-κB (p50, p65), IKK-α, phospho-IκB-α and inflammation-related proteins, COX-2, iNOS, IL-1β and VCAM-1, were examined by western blot. The fluorescent probes, 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihyldrorhodamine 123 (DHR 123) were used to estimate the scavenging effect of Prunellae Spica on ․O₂-, NO, ONOO-. Western blot was conducted to assess the protein expression levels of NF-κB (p50, p65), IKK-α, phospho-IκB-α, inflammation-related proteins, COX-2, iNOS, IL-1β, VCAM-1. PH inhibited H₂O₂-treated cell death dose-dependently. It reduced the generation of ·O2-, NO, ONOO- and PGE₂ in the H₂O₂-treated renal epitheial cells(LLC-PK1 cell) of mouse in vitro. PH reduced the expression of NF-κB, IKK-α, phospho-IκB-α, COX-2, iNOS, IL-1β and VCAM-1 genes through means of decreasing activation of NF-κB signaling as well. According to these results, PH has an antioxidative activity and anti-inflammatory effect by regulating the NF-κB pathway. This suggest that PH is expected to be used to regulating inflammatory process and treating inflammation-related disease.

#Prunellae Herba #Reactive oxygen species #Reactive nitrogen species #NF-κB #Redox Status #anti-inflammatory