Abstract
Alginates are composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G). In this study, block distributions of alginate samples were measured by a chemical fractionation method and 1H-NMR spectroscopy, to compare the usefulness of the two methods. Block distribution results obtained from the two methods were found to be statistically different from each other; the chemical fractionation method gave consistently lower estimates for the amount of homopolymeric blocks. The repeatability of the results was also lower in the chemical fractionation method. By contrast, 1H-NMR spectroscopy was found to be a reproducible and a faster method for determining the distribution of monomeric blocks in alginates.
This work was supported by the Turkish Prime Ministry-State Planning Organization and METU Research Fund (BAP-DPT 2002K120510). We would like to thank FMC Biopolymer for providing the alginate samples for our study.
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