Abstract
Background:
Despite the acclaimed beneficial effects of Telfaria occidentalis (TO), it is yet to be established that its aqueous extract is safe in the condition of renal impairment. Thus, the study investigated the effects of TO aqueous leaves extract on gentamycin-induced renal damage.
Methods:
The animals were distributed into five groups. Group A (control) was placed on standard rat feed. Groups B and C received 500 mg/kg and 80 mg/kg of TO and gentamicin for 21 days, respectively. Group D received 500 mg/kg of TO 14 days before 7 days administration of 80 mg/kg of gentamycin. Group E received 80 mg/kg of gentamicin for 14 days before 7 days administration of 500 mg/kg TO. Group F received 500 mg/kg of TO and 80mg/kg of gentamycin concurrently for 21 days. Biochemical and histological examinations were analysed by standard methods.
Results:
The administration of TO for 7 days after 14 days of gentamycin injection and its concomitant administration with gentamicin for 21 days caused significant reduction (p<0.05) on the relative kidney weight, creatinine and uric acid levels compared to groups C and D. There was a significant decrease (p<0.05) in the mean serum potassium level in group C compared to groups A, B, D, and F. The histological reports showed that the combination of the extract and gentamycin (group F) seems to ameliorate the deleterious effect observed when gentamycin was administered alone.
Conclusions:
The administration of the extract together with and after the administration of gentamycin reverses renal damage caused by gentamycin.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Oshikoya KA, Senbanjo IO, Njokanma OF, Soipe A. Use of complementary and alternative medicines for children with chronic health conditions in Lagos Nigeria. BMC Complement Altern Med 2008;8:66.10.1186/1472-6882-8-66Search in Google Scholar PubMed PubMed Central
2. Yusuff KB, Tayo F. Frequency, types and severity of medication use-related problems among medical outpatients in Nigeria. Int J Clin Pharm 2011;33:558–64.10.1007/s11096-011-9508-zSearch in Google Scholar PubMed
3. King AR, Flint S, Russett FS, Generali JA, Grauer DW. Evaluation and implications of natural product use in preoperative patients: a retrospective review. BMC Complement Altern Med 2009;9:38.10.1186/1472-6882-9-38Search in Google Scholar PubMed PubMed Central
4. Collins D, Oakey S, Ramakrishnan V. Perioperative use of herbal, complementary and over the counter medicines in plastic surgery patients. Eplasty 2011;11:e27.Search in Google Scholar
5. Ezejiofor NA, Maduagwunan C, Onyiaorah VI, Hussaini DC, Orisakwe OE. Multiple organ toxicity of a Nigerian herbal supplement [U and D sweet bitter] in male albino rats. Pak J Pharm Sci 2008;21:426–9.Search in Google Scholar
6. Sheikh NM, Philen RM, Love LA. Chaparral-associated hepatotoxicity. Arch Internal Med 1997;157:913–9.10.1001/archinte.1997.00440290099011Search in Google Scholar
7. Kayode AA, Kayode OT. Some medicinal values of Telfairia occidentalis: a review. Am J Biochem Molec Biol 2011;1:30–8.10.3923/ajbmb.2011.30.38Search in Google Scholar
8. Obaji NN, Egwurugwu JN, Ifepe IC, Dimkpa U. Comparative study on the effects of crude extracts of Pterocarpus Soyauxii and Telfairia occidentalis on haematological parameters of Albino rats. Inter J Tropic Agric Food Syst 2010;4:247–51.Search in Google Scholar
9. Kayode AA, Kayode OT, Adetola AA. Telfairia occidentalis ameliorates oxidative brain damage in malnourished rat. Int J Biol Chem 2010;4:10–8.10.3923/ijbc.2010.10.18Search in Google Scholar
10. Omale J, Okafor PN. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. Afr J Biotechnol 2008;7:3129–33.Search in Google Scholar
11. Iweala EE, Obidoa O. Some biochemical, haematological and histological responses to a long term consumption of Telfairia occidentalis-supplemented diet in rats. Pak J Nutr 2009;8:1199–03.10.3923/pjn.2009.1199.1203Search in Google Scholar
12. Salman TM, Isiaka AA, Sikiru AB, Olusegun AA, Opeyemi KO, Olanrewaju AA. Blood glucose-lowering effect of Telfairia occidentalis: a preliminary study on the underlying mechanism and responses. Biokemistri 2012;25:133–9.Search in Google Scholar
13. Ojo NA, Adawaren EO, Tijjani M B, Chiroma M, Simon J, Afisu B, et al. Acute toxicity and effect of ethanolic extract of Tefairia occidentalis leaves on blood glucose level in normal rats. Vom J Vet Sci 2012;9:25–31.Search in Google Scholar
14. Ugwu CE, Olajid JE, Alumana EO, Ezeanyika LU. Comparative effects of the leaves of Vernonia amygdalina and Telfairia occidentalis incorporated diets on the lipid profile of rats. Afr J Biochem Res 2011;5:28–32.Search in Google Scholar
15. Adisa WA, Okhiai O, Bankole JK, Iyamu OA, Aigbe O. Testicular damage in Telfairia occidentalis extract treated Wistar rats. Am J Med Biol Res 2014;2:37–45.10.12691/ajmbr-2-2-2Search in Google Scholar
16. Saalu LC, Kpela T, Benebo AS, Oyewopo AO, Anifowope EO, Oguntola JA. The dose-dependent testiculoprotective and testiculotoxic potentials of Telfairia occidentalis Hook f. leave extract in rat. Inter J Appl Res Natr Prod 2010;3:27–38.Search in Google Scholar
17. Moulds R. Gentamicin: a great way to start. Austr Prescrib 2010;33:134–5.10.18773/austprescr.2010.062Search in Google Scholar
18. Sundin DP, Sandoval R, Molitoris BA. Gentamicin inhibits renal protein and phospholipid metabolism in rats: implications involving intracellular trafficking. J Am Soc Nephrol 2001;12:114–23.10.1681/ASN.V121114Search in Google Scholar PubMed
19. Raveh D, Kopyt M, Hite Y, Rudensky B, Sonnenblick M, Yinnon AM. Risk factors for nephrotoxicity in elderly patients receiving once-daily aminoglycosides. Q J Med 2002;95:291–7.10.1093/qjmed/95.5.291Search in Google Scholar
20. Balakumar P, Rohilla A, Thangathirupathi A. Gentamicin-induced nephrotoxicity: do we have a promising therapeutic approach to blunt it? Pharmacol Res 2010;62:179–86.10.1016/j.phrs.2010.04.004Search in Google Scholar
21. Luyckx VA, Ballantine R, Claeys M, Cuyckens F, Vanden Heuvel H, Cimanga RK. Herbal remedy – associated acute renal failure secondary to cape aloes. Am J Kidney Dis 2002;39:E13.10.1053/ajkd.2002.31424Search in Google Scholar
22. Ekpenyong CE, Akpan EE, Udoh NS. Phytochemistry and toxicity studies of Telfairia occidentalis aqueous leaves extract on liver biochemical indices in wistar rats. AJMMS 2012;2:103–10.10.5923/j.ajmms.20120205.03Search in Google Scholar
23. Mumtaz F, Khaliq T, Javed I, Iftikhar A, Aslam B, Ali A, et al. Effects of Rosa damascena mill flowers, Cichorium intybus linn roots and their mixtures on serum electrolytes and hematological parameters against gentamicin induced toxicity in albino rabbits. Indo Am J Pharm Res 2014;4:236–42.Search in Google Scholar
24. Farag MM, Mikhail M, Shehata R, Abdel-Meguid E, Abdel-Tawab S. Assessment of gentamicin induced nephrotoxicity in rats treated with low doses of ibuprofen and diclofenac sodium. J Clin Sci (Lond) 1996;91:187–9.10.1042/cs0910187Search in Google Scholar
25. Aysen E, Nimet UG, Usubutun A, Kamer K, Remzi SE, Aysun K, et al. The protective effect of taurine against gentamicin induced acute tubular necrosis in rats. Nephrol Dial Transplant 2000;15:1175–82.10.1093/ndt/15.8.1175Search in Google Scholar
26. Ekpenyong CE, Okon UA, Samson TK, Akpan EE. Bioactive constituents, acute and sub-acute effect of Telfairia occidentalis hook f (fluted pumpkin) leaves extract on some kidney function indices in rat. Can J Pure Appl Sci 2012;6:2105–12.Search in Google Scholar
27. Asif AH, Rasool ST, Khan TM. Pyridoxal phosphate a possible intervention to prevent aminoglycoside induced electrolyte imbalance. J Clin Res Bioeth 2012;3:124.10.4172/2155-9627.1000124Search in Google Scholar
28. Chen LC, Yan SC, Min TS, Chia KL, Han WH. Gentamicin induced serum electrolytes abnormalities. Med Sci 1984;5:1997–2002.Search in Google Scholar
29. Kojima R, Ito M, Suzuki Y. Studies on the nephrotoxicity of aminoglycoside antibiotics and protection from these effects (8): protective effect of pyridoxal-5′-phosphate against tobramycin nephrotoxicity. Jpn J Pharmacol 1990;52:11–21.10.1016/S0021-5198(19)37763-7Search in Google Scholar
30. Pramila M, Padmini I, Vijay KJ. A histopathological study on gentamycin induced nephrotoxicity in experimental Albino rats. J Dental Med Sci 2013;1:14–7.10.9790/0853-0111417Search in Google Scholar
31. Ullah N, Khan MA, Khan T, Ahmad W. Protective effect of Cinnamomum tamala extract on gentamicin-induced nephrotic damage in rabbits. Tropic J Pharm Res 2013;12:215–9.10.4314/tjpr.v12i2.13Search in Google Scholar
32. Ihab TA, Ahmed AA, Gamal AM. Protective effect of quercetin against gentamicin-induced nephrotoxicity in rats. Biol Pharm Bull 2009;32:61–7.10.1248/bpb.32.61Search in Google Scholar PubMed
33. Fatemeh GA, Mahmoud R, Mehdi N, Azar B, Hamid N. Ameliorative effects of metformin on renal histologic and biochemical alterations of gentamicin-induced renal toxicity in Wistar rats. J Res Med Sci 2012;17:621–5.Search in Google Scholar
34. Arunachalam K, Suchetha KN, Prima D, Divya B. Evaluation of renal protective activity of Adhatoda zeylanica (medic) leaves extract in wistar rats. Nitte Uni J Health Sc 2013;3:45–56.Search in Google Scholar
35. Karadeniz A, Yildirim A, Simsek N, Kalkan Y, Celebi F. Spirulina platensis protects against gentamicin-induced nephrotoxicity in rats. Phytother Res 2008;22:1506–10.10.1002/ptr.2522Search in Google Scholar
36. Nitha B, Janardhanan KK. Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin-induced nephrotoxicity in mice. Food Chem Toxicol 2008;46:3193–9.10.1016/j.fct.2008.07.007Search in Google Scholar
37. Kalayarasan S, Prabhu PN, Sriram N, Manikandan R, Arumugam M, Sudhandiran G. Diallyl sulfide enhances antioxidants and inhibits inflammation through the activation of Nrf2 against gentamicin induced nephrotoxicity in wistar rats. Eur J Pharmacol 2009;606:162–71.10.1016/j.ejphar.2008.12.055Search in Google Scholar
38. Shahidi F, Wanasundara PK. Phenolic antioxidant. Crit Rev Food Sci Nutr 1992;32:67–103.10.1080/10408399209527581Search in Google Scholar
39. Brand-Williams W, Givelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25–30.10.1016/S0023-6438(95)80008-5Search in Google Scholar
40. Kayode OT, Kayode AA, Adetola AA. Therapeutic effect of Telfairia occidentalis on protein energy malnutrition-induced liver damage. Res J Med Plant 2009;3:80–92.10.3923/rjmp.2009.80.92Search in Google Scholar
41. Majid T, Hasan A, Pooran T. Inhibitory effect of olive leaf extract on gentamicin-induced nephrotoxicity in rats. Iran J Kidney Dis 2012;6:25–32.Search in Google Scholar
42. Saeed CA, Mostafa J, Houshang N, Mahboubeh A. Protective effects of Salvia officinalis extract against gentamicin-induced nephrotoxicity in rat. J Kermanshah Univ Med Sci 2014;17:212–20.Search in Google Scholar
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