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Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans

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Abstract

The aim of the study was to assess the effects of low-temperature hardening (2°C) on the biochemical compounds and processes that can increase resistance of winter rape to inoculation with Leptosphaeria maculans spores. The study involved an evaluation of the entire pool of phenolic compounds, L-phenylalanine ammonia lyase (PAL) activity, excitation intensity for blue and green fluorescence, catalase (CAT) activity, respiration intensity and heat emission from leaf tissues. All the measurements were performed 24 and 72 hours after the inoculation. Low-temperature hardening, which preceded the inoculation of rape seedlings with spores of L. maculans, caused a significant increase in CAT activity and the level of phenolic compounds. The observed changes in PAL activity reflected the changes in phenolics content. The hardened plants showed a significantly higher intensity of blue fluorescence excitation at 24 and 72 hours after the inoculation, as compared to the non-hardened seedlings. Increased content of phenolic compounds and PAL and catalase activity triggered by the temperature of 2 °C and maintained for 24 hours after the inoculation, may confirm the stimulating effect of the hardening temperature. Intensified emission of blue fluorescence indicating saturation of a cell wall with phenolic compounds makes the cell wall structure less stretchy, more tight and leakproof, and thereby hinders fungal growth through plant tissue.

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Abbreviations

CAT:

catalase

F430:

blue fluorescence

F520:

green fluorescence

HE:

heat emission

LWC:

leaf water content

PAL:

phenylalanine ammonia lyase

PPFD:

photosynthetic photon flux density

Raw4:

isolate of L. maculans

RI:

respiration intensity

ROS:

reactive oxygen species

SPh:

soluble phenolics

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Agriculture and Economics, Agricultural University, Podłużna 3, 30-239, Kraków, Poland

    Katarzyna Hura, Marcin Rapacz & Agnieszka Płażek

  2. The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland

    Tomasz Hura

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  1. Katarzyna Hura
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  2. Tomasz Hura
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  4. Agnieszka Płażek
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Correspondence to Katarzyna Hura.

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Hura, K., Hura, T., Rapacz, M. et al. Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans. Biologia 70, 1011–1018 (2015). https://doi.org/10.1515/biolog-2015-0129

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