Hairy roots of Hyssopus officinalis L. were induced by infection of petioles with Agrobacterium rhizogenes LBA 9402 and studied for production of phenolic acids, especially rosmarinic acid (RA). The highest content of rosmarinic acid (about 6 % of dry weight) was obtained in hairy roots grown in Gamborg’s B5 liquid medium containing 10% (w/v) sucrose. The level was at least 60% higher than those found in callus, cell suspension culture and roots of one-year-old field grow n plants. Apart from RA , nine other phenolic acids were detected in transformed roots and quantified by gas chromatography
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