Abstract
The data on hormonal steroids in the human seminal plasma and their role in spermatogenesis are summarized. The seminal steroid levels need not correlate with the blood plasma levels. The recent reports showed that androgen, especially dihydrotestosterone, and the estrogen levels in the seminal fluid may be used as the markers of spermatogenesis impairment. The estradiol concentration in the seminal plasma was higher than in the blood plasma, and its levels were significantly increased in men with impaired spermatogenesis. A good indicator for predicting the normal spermatogenesis, therefore, seems to be the testosterone/estradiol ratio. The seminal plasma also contains significant amounts of cortisol, which influences the androgen biosynthesis through its receptors in the Leydig cells. The local balance between cortisol and inactive cortisone is regulated by 11β-hydroxysteroid dehydrogenase, the activity of which may be affected by the environmental chemicals acting as the endocrine disruptors (EDCs). These compounds are believed to participate in worsening the semen quality – the sperm count, motility, and morphology, as witnessed in the recent last decades. As to the steroids’ role in the testis, the EDCs may act as antiandrogens by inhibiting the enzymes of testosterone biosynthesis, as the agonists or antagonists through their interaction with the steroid hormone receptors, or at the hypothalamic-pituitary-gonadal axis. Surprisingly, though the EDCs affect the steroid action in the testis, there is no report of a direct association between the concentrations of steroids and the EDCs in the seminal fluid. Therefore, measuring the steroids in the semen, along with the various EDCs, could help us better understand the role of the EDCs in the male reproduction.
This work was supported by Grant Project No. 13369-4 from the Internal Grant Agency of the Czech Ministry of Health.
References
1. Purvis K, Landgren BM, Cekan Z, Diczfalusy E. Indices of gonadal function in the human male. II. Seminal plasma levels of steroids in normal and pathological conditions. Clin Endocrinol (Oxf) 1975;4:247–58.10.1111/j.1365-2265.1975.tb01532.xSearch in Google Scholar
2. Adamopoulos DA, Lawrence DM, Swyer GI. Determination of testosterone concentration in semen of men with normal or subnormal sperm counts and after vasectomy. Acta Eur Fertil 1976;7:219–25.Search in Google Scholar
3. De Aloysio D, D’Urso N, Giardini G, Nicoletti G, Gori G. Plasma and semen androgens in normospermic and dispermic subjects. Acta Eur Fertil 1978;9:139–44.Search in Google Scholar
4. Pazzagli M, Giusti G, Forti G, Fiorelli G, Menchini-Fabris F, Scarselli GF, Guazzelli R, Conti C, Borrelli D, Cicchi P, Serio M. Seminal plasma levels of testosterone and 5alpha-dihydrotestosterone in azoospermic patients. Clin Endocrinol (Oxf) 1979;11:11–4.10.1111/j.1365-2265.1979.tb03042.xSearch in Google Scholar
5. Le Lannou D, Massart C, Chambon Y, Nicol M, Allannic H. Testosterone and 5 alpha-dihydrotestosterone concentrations in human seminal plasma. Int J Androl 1980;3:502–6.10.1111/j.1365-2605.1980.tb00138.xSearch in Google Scholar
6. Moreno-Escallon B, Ridley AJ, Wu CH, Blasco L. Hormones in seminal plasma. Arch Androl 1982;9:127–34.10.3109/01485018208990230Search in Google Scholar
7. García Díez LC, Gonzalez Buitrago JM, Corrales JJ, Battaner E, Miralles JM. Hormone levels in serum and seminal plasma of men with different types of azoospermia. J Reprod Fertil 1983;67:209–14.10.1530/jrf.0.0670209Search in Google Scholar
8. Kurniawan E, Tamm J, Volkwein U, Schirren C. Unconjugated 5 alpha-androstan-3 alpha, 17 beta-diol and 5 alpha-androstane-3 beta, 17 beta-diol in normal and pathological human seminal plasma. Comparison with testosterone, 5 alpha-dihydrotestosterone and testosterone-glucosiduronate. Andrologia 1983;5:141–50.Search in Google Scholar
9. Adamopoulos D, Lawrence DM, Vassilopoulos P, Kapolla N, Kontogeorgos L, McGarrigle HH. Hormone levels in the reproductive system of normospermic men and patients with oligospermia and varicocele. J Clin Endocrinol Metab 1984;59:447–552.10.1210/jcem-59-3-447Search in Google Scholar
10. Singer R, Sagiv M, Bruchis S, Barnet M, Kaufman H, Servadio C. Total and free testosterone and estradiol in human semen. Int J Fertil 1987;32:145–8.Search in Google Scholar
11. Facchinetti F, Comitini G, Genazzani A, Bakalakis C, Genazzani AR, Loche S. Seminal fluid androgen levels in infertile patients. Int J Fertil 1987;32:157–61.Search in Google Scholar
12. Bujan L, Mieusset R, Audran F, Lumbroso S, Sultan C. Increased oestradiol level in seminal plasma in infertile men. Hum Reprod 1993;8:74–7.10.1093/oxfordjournals.humrep.a137878Search in Google Scholar
13. Zalata A, Hafez T, Verdonck L, Vermeulen L, Comhaire F. Androgens in seminal plasma: markers of the surface epithelium of the male reproductive tract. Int J Androl 1995;18:271–7.10.1111/ijan.1995.18.5.271Search in Google Scholar
14. Laudat A, Guechot J, Palluel AM. Seminal androgen concentrations and residual sperm cytoplasm. Clin Chim Acta 1998;276:11–8.10.1016/S0009-8981(98)00090-4Search in Google Scholar
15. Luboshitzky R, Shen-Orr Z, Herer P. Seminal plasma melatonin and gonadal steroids concentrations in normal men. Arch Androl 2002;8:225–32.10.1080/01485010252869324Search in Google Scholar PubMed
16. Zhang Q, Bai Q, Yuan Y, Liu P, Qiao J. Assessment of seminal estradiol and testosterone levels as predictors of human spermatogenesis. J Androl 2010;31:215–20.10.2164/jandrol.109.007609Search in Google Scholar
17. Wilson JD, Foster DW, Kronenberg HM, Larsen PR, editors. Williams textbook of endocrinology, 9th ed. Philadelphia: WB Saunders Co, 1998.Search in Google Scholar
18. Schwartz JI, Tanaka WK, Wang DZ, Ebel DL, Geissler LA, Dallob A, Hafkin B, Gertz BJ. MK-386, an inhibitor of 5alpha-reductase type 1, reduces dihydrotestosterone concentrations in serum and sebum without affecting dihydrotestosterone concentrations in semen. J Clin Endocrinol Metab 1997;82:1373–7.Search in Google Scholar
19. Santiemma V, Rosati P, Fazzi V, Bolelli GF, Guerzoni C, Fabbrini A. Seminal estrone, estrone sulfate, and estradiol-17 beta levels in fertile and infertile males. Arch Androl 1991;26:129–34.10.3109/01485019108987635Search in Google Scholar
20. Abbaticchio G, Giorgino R, Urago M, Gattuccio F, Orlando G, Jannì A. Hormones in the seminal plasma. Cortisol. Acta Eur Fertil 1981;12:239–44.Search in Google Scholar
21. Brotherton J. Cortisol and transcortin in human seminal plasma and amniotic fluid as estimated by modern specific assays. Andrologia 1990;22:197–204.10.1111/j.1439-0272.1990.tb01966.xSearch in Google Scholar
22. Hampl R, Pohanka M, Hill M, Stárka L. The content of four immunomodulatory steroids and major androgens in human semen. J Steroid Biochem Mol Biol 2003;84:307–16.10.1016/S0960-0760(03)00044-XSearch in Google Scholar
23. Dong Q, Salva A, Sottas CM, Niu E, Holmes M, Hardy MP. Rapid glucocorticoid mediation of suppressed testosterone biosynthesis in male mice subjected to immobilization stress. J Androl 2004;25:973–81.10.1002/j.1939-4640.2004.tb03170.xSearch in Google Scholar PubMed
24. Hu GX, Lian QQ, Lin H, Latif SA, Morris DJ, Hardy MP, Ge RS. Rapid mechanisms of glucocorticoid signaling in the Leydig cell. Steroids 2008;73:1018–24.10.1016/j.steroids.2007.12.020Search in Google Scholar PubMed PubMed Central
25. Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50 years. Br Med J 1992;305:609–13.10.1136/bmj.305.6854.609Search in Google Scholar PubMed PubMed Central
26. Auger J, Kunstmann JM, Czyglik F, Jouannet P. Decline in semen quality among fertile men in Paris during the past 20 years. N Engl J Med 1995;332:281–5.10.1056/NEJM199502023320501Search in Google Scholar PubMed
27. Feki NC, Abid N, Rebai A, Sellami A, Ayed BB, Guermazi M, Bahloul A, Rebai T, Ammar LK. Semen quality decline among men in infertile relationships: experience over 12 years in the South of Tunisia. J Androl 2009;30:541–7.10.2164/jandrol.108.005959Search in Google Scholar PubMed
28. Paulsen CA, Berman NG, Wang C. Data from men in greater Seattle area reveals no downward trend in semen quality: further evidence that deterioration of semen quality is not geographically uniform. Fertil Steril 1996;65:1015–20.10.1097/00006254-199609000-00018Search in Google Scholar
29. Fisch H, Goluboff ET, Olson JH, Feldshuh J, Broder SJ, Barad DH. Semen analyses in 1,283 men from the United States over a 25-year period: no decline in quality. Fertil Steril 1996;65: 1009–14.10.1016/S0015-0282(16)58278-8Search in Google Scholar
30. Sripada S, Fonseca S, Lee A, Harrild K, Giannaris D, Mathers E, Bhattacharya S. Trends in semen parameters in the northeast of Scotland. J Androl 2007;28:313–9.10.2164/jandrol.106.000729Search in Google Scholar PubMed
31. Merzenich H, Zeeb H, Blettner M. Decreasing sperm quality: a global problem? BMC Public Health 2010;10:24.10.1186/1471-2458-10-24Search in Google Scholar PubMed PubMed Central
32. Mathur PP, D’Cruz SC. The effect of environmental contaminants on testicular function. Asian J Androl 2011;13:585–91.10.1038/aja.2011.40Search in Google Scholar PubMed PubMed Central
33. Phillips KP, Tanphaichitr N. Human exposure to endocrine disrupters and semen quality. J Toxicol Environ Health B Crit Rev 2008;11:188–220.10.1080/10937400701873472Search in Google Scholar PubMed
34. Hauser R, Barthold JS, Meeker JD. Epidemiologic evidence on the relationship between environmental endocrine disruptors and male reproductive and developmental health. In: Gore AC, editor. Endocrine disrupting chemicals: from basic research to clinical practice. Totowa, NJ: Humana Press Inc, 2010:225–51.Search in Google Scholar
35. Ye L, Su ZJ, Ge RS. Inhibitors of testosterone biosynthetic and metabolic activation enzymes. Molecules 2011;16: 9983–10001.10.3390/molecules16129983Search in Google Scholar PubMed PubMed Central
36. Tabb MM, Blumberg B. New modes of action for endocrine-disrupting chemicals. Mol Endocrinol 2006;20:475–82.10.1210/me.2004-0513Search in Google Scholar PubMed
37. Rey RA, Musse M, Venara M, Chemes HE. Ontogeny of the androgen receptor expression in the fetal and postnatal testis: its relevance on sertoli cell maturation and the onset of adult spermatogenesis. Microsc Res Tech 2009;72:787–95.10.1002/jemt.20754Search in Google Scholar PubMed
38. Martin LJ, Tremblay JJ. Nuclear receptors in Leydig cell gene expression and function. Biol Reprod 2010;83:3–14.10.1095/biolreprod.110.083824Search in Google Scholar PubMed PubMed Central
39. Cavaco JE, Laurentino SS, Barros A, Sousa M, Socorro S. Estrogen receptors alpha and beta in human testis: both isoforms are expressed. Syst Biol Reprod Med 2009;55:137–44.10.3109/19396360902855733Search in Google Scholar PubMed
40. Schoeters G, Den Hond E, Dhooge W, van Larebeke N, Leijs M. Endocrine disruptors and abnormalities of pubertal development. Basic Clin Pharmacol Toxicol 2008;102:168–75.10.1111/j.1742-7843.2007.00180.xSearch in Google Scholar
41. Meeker JD, Yang T, Ye X, Calafat AM, Hauser R. Urinary concentrations of parabens and serum hormone levels, semen quality parameters, and sperm DNA damage. Environ Health Perspect 2011;119:252–7.10.1289/ehp.1002238Search in Google Scholar
42. Meeker JD, Ehrlich S, Toth TL, Wright DL, Calafat AM, Trisini AT, Ye X, Hauser R. Semen quality and sperm DNA damage in relation to urinary bisphenol A among men from an infertility clinic. Reprod Toxicol 2010;30:532–9.10.1016/j.reprotox.2010.07.005Search in Google Scholar
43. Meeker JD, Hauser R. Exposure to polychlorinated biphenyls (PCBs) and male reproduction. Syst Biol Reprod Med 2010;56:122–31.10.3109/19396360903443658Search in Google Scholar
44. Adlercreutz H. Phyto-oestrogens and cancer. Lancet Oncol 2002;3:364–73.10.1016/S1470-2045(02)00777-5Search in Google Scholar
45. Messina M. Soybean isoflavone exposure does not have feminizing effects on men: a critical examination of the clinical evidence. Fertil Steril 2010;93:2095–104.10.1016/j.fertnstert.2010.03.002Search in Google Scholar PubMed
46. Mitchell JH, Cawood E, Kinniburgh D, Provan A, Collins AR, Irvine DS. Effect of a phytoestrogen food supplement on reproductive health in normal males. Clin Sci (Lond) 2001;100:613–8.10.1042/CS20000212Search in Google Scholar
47. Giwercman A. Estrogens and phytoestrogens in male infertility. Curr Opin Urol 2011;21:519–26.10.1097/MOU.0b013e32834b7e7cSearch in Google Scholar PubMed
48. Cederroth CR, Auger J, Zimmermann C, Eustache F, Nef S. Soy, phyto-oestrogens and male reproductive function: a review. Int J Androl 2010;33:304–16.10.1111/j.1365-2605.2009.01011.xSearch in Google Scholar PubMed
49. Rogatsky I, Ivashkiv LB. Glucocorticoid modulation of cytokine signaling. Tissue Antigens 2006;68:1–12.10.1111/j.1399-0039.2006.00599.xSearch in Google Scholar PubMed
50. Flammer JR, Rogatsky I. Minireview: glucocorticoids in autoimmunity: unexpected targets and mechanisms. Mol Endocrinol 2011;25:1075–86.10.1210/me.2011-0068Search in Google Scholar
51. Ratman D, Berghe WV, Dejager L, Libert C, Tavernier J, Beck IM, De Bosscher K. How glucocorticoid receptors modulate the activity of other transcription factors: a scope beyond tethering. Mol Cell Endocrinol 2013, in press.10.1016/j.mce.2012.12.014Search in Google Scholar
52. Seshadri S, Bates M, Vince G, Jones DI. Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle. Andrologia 2011;43:378–86.10.1111/j.1439-0272.2010.01042.xSearch in Google Scholar
53. Qian L, Sun G, Zhou B, Wang G, Song J, He H. Study on the relationship between different cytokines in the semen of infertility patients. Am J Reprod Immunol 2011;66:157–61.10.1111/j.1600-0897.2010.00980.xSearch in Google Scholar
54. Ge RS, Dong Q, Niu EM, Sottas CM, Hardy DO, Catterall JF, Latif SA, Morris DJ, Hardy MP. 11{beta}-Hydroxysteroid dehydrogenase 2 in rat leydig cells: its role in blunting glucocorticoid action at physiological levels of substrate. Endocrinology 2005;146:2657–64.10.1210/en.2005-0046Search in Google Scholar
55. Nacharaju VL, Muneyyirci-Delale O, Khan N. Presence of 11 beta-hydroxysteroid dehydrogenase in human semen: evidence of correlation with semen characteristics. Steroids 1997;62:311–4.10.1016/S0039-128X(96)00225-5Search in Google Scholar
56. Ohshima M, Ohno S, Nakajin S. Inhibitory effects of some possible endocrine-disrupting chemicals on the isozymes of human 11beta-hydroxysteroid dehydrogenase and expression of their mRNA in gonads and adrenal glands. Environ Sci 2005;12:219–30.Search in Google Scholar
57. Ma X, Lian QQ, Dong Q, Ge RS. Environmental inhibitors of 11β-hydroxysteroid dehydrogenase type 2. Toxicology 2011;285:83–9.10.1016/j.tox.2011.04.007Search in Google Scholar
58. Rozati R, Reddy PP, Reddanna P, Mujtaba R. Role of environmental estrogens in the deterioration of male factor fertility. Fertil Steril 2002;78:1187–94.10.1016/S0015-0282(02)04389-3Search in Google Scholar
59. Mazzeo P, Di Pasquale D, Ruggieri F, Fanelli M, D’Archivio AA, Carlucci G. HPLC with diode-array detection for the simultaneous determination of di(2-ethylhexyl)phthalate and mono(2-ethylhexyl)phthalate in seminal plasma. Biomed Chromatogr 2007;21:1166–71.10.1002/bmc.870Search in Google Scholar PubMed
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