Abstract
Background: Preclinical and clinical studies suggest the use of antioxidants as an effective measure to reduce the progression of oxidative-stress-related disorders. Nuclear factor E2-related factor 2 (Nrf2) is a key component to cellular redox homeostasis in the attenuation of oxidative-stress-associated pathological processes. The objective of the current study was to evaluate the role of geraniol (GOH) in preserving the plasma lipid status, endothelial function, antioxidant status, and inhibition of lipid peroxidation (LPO) in hamsters fed an atherogenic diet (AD).
Methods: Male Syrian hamsters were randomly grouped into four groups: group 1 was control animals; group 2 was animals fed GOH alone (100 mg/kg bw po); group 3 was animals fed AD (standard pellet diet+10% coconut oil+0.25% cholesterol+0.25% cholic acid); and group 4 was fed AD+GOH (100 mg/kg bw) for 12 weeks. At the end of the feeding period, the animals were sacrificed and the liver, heart, and aorta from each group were analyzed for antioxidants, LPO markers, and histological changes.
Results: AD feeding induced a significant change in lipid profile, endothelial function marker, activities of the antioxidant enzymes, alterations in the LPO markers, Nrf2 expression, and equally significant changes in the organ histology.
Conclusions: Supplementation with GOH appreciably prevented the alterations induced by the AD on all the above parameters. Thus, GOH offers marked protection against AD-induced abnormalities.
References
1. World Health Organization. Global atlas on cardiovascular disease prevention and control. http://www.Who.Int/cardiovasculardiseases/en/. Accessed September 23, 2011.Search in Google Scholar
2. Edris AE. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res 2007;21:308–23.10.1002/ptr.2072Search in Google Scholar
3. Buhler DR, Miranda C. Antioxidant activities of flavonoids. Oregon: Oregon State University, 2000.Search in Google Scholar
4. Agbor GA, Oben JE, Ngogang JY, Xinxing C, Vinson JA. Antioxidant capacity of some herbs/spices from Cameroon a comparative study of two methods. J Agric Food Chem 2005;53:6819–24.10.1021/jf050445cSearch in Google Scholar
5. Vinson JA, Proch J, Bose P, Muchler S, Taffera P, Shuta D, et al. Chocolate is a powerful ex vivo and in vivo antioxidant, an antiatherosclerotic agent in an animal model, and a significant contributor to antioxidants in the European and American diets. J Agric Food Chem 2006;54:8071–6.10.1021/jf062175jSearch in Google Scholar
6. Decorde K, Teissedre PL, Sutra T, Chardonnay T. Grape seed procyanidin extract supplementation prevents high diet induced obesity in hamsters by improving adipolane imbalance and oxidative stress markers. Mol Nutr Food Res 2009;53:659–66.10.1002/mnfr.200800165Search in Google Scholar
7. Agbor GA, Vinson JA, Sortino J, Johnson R. Antioxidant and antiatherogenic activities of three GOH species on atherogenic diet fed hamsters. Exp Toxicol Pathol 2012;64:387–91.10.1016/j.etp.2010.10.003Search in Google Scholar
8. Casino PR, Kilcoyne CM, Quyyumi AA, Hoeg JM, Panza JA. The role of nitric oxide in endothelium-dependent vasodilation of hypercholesterolemic patients. Circulation 1993;88:2541–7.10.1161/01.CIR.88.6.2541Search in Google Scholar
9. Slocum SL, Kensler TW. Nrf2: control of sensitivity to carcinogens. Arch Toxicol 2011;854:273–84.10.1007/s00204-011-0675-4Search in Google Scholar
10. Shoff SM, Grummer M, Yatvin MB, Elson CE. Concentration-dependent increase of murine P388 and B16 population doubling time by the acyclic monoterpene GOH. Cancer Res 1991;51:37–42.10.1016/0304-3835(90)90228-PSearch in Google Scholar
11. Carnesecchi S, Schneider Y, Ceraline J, Duranton B, Gosse F, Seiler N, et al. GOH, a component of plant essential oils, inhibits growth and polyamine biosynthesis in human colon cancer cells. J Pharmacol Exp Ther 2001;298:197–200.Search in Google Scholar
12. Carnesecchi S, Langley K, Exinger F, Gosse F, Raul F. GOH, a component of plant essential oils, sensitizes human colonic cancer cell to 5-fluorouracil treatment. J Pharmacol Exp Ther 2002;301:1–6.10.1124/jpet.301.2.625Search in Google Scholar
13. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrite and nitrate in biological fluids. Anal Biochem 1982;126:131–8.10.1016/0003-2697(82)90118-XSearch in Google Scholar
14. Fraga CG, Leibouitz BE, Toppel AL. Lipid peroxidation measured as TBARS in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 1988;4:155–61.10.1016/0891-5849(88)90023-8Search in Google Scholar
15. Jiang ZY, Hunt JV, Wolff SP. Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxides in low density lipoprotein. Anal Biochem 1993;202:384.10.1016/0003-2697(92)90122-NSearch in Google Scholar
16. Esterbauer H, Striegl G, Puhl H, Rotheneder M. Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun 1989;6:67–75.10.3109/10715768909073429Search in Google Scholar
17. Tiwari M, Kakkar P. Plant derived antioxidants – GOH and camphene protect rat alveolar macrophages against t-BHP induced oxidative stress. Toxicol In Vitro 1999;23:295–301.10.1016/j.tiv.2008.12.014Search in Google Scholar
18. Sun GY, Horrocks LA, Farooqui AA. The roles of NADPH oxidase and phospholipases A2 in oxidative and inflammatory responses in neurodegenerative diseases. J Neurochem 2007;103:1–16.10.1111/j.1471-4159.2007.04670.xSearch in Google Scholar
19. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389–94.10.1016/0003-2697(72)90132-7Search in Google Scholar
20. Beers RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195:133–40.10.1016/S0021-9258(19)50881-XSearch in Google Scholar
21. Rotruck JT, Pope AL, Ganther H, Awanson AB, Hafeman DG, Hoeckstra WG. Selenium biochemical role as a component of glutathione peroxidase. Science 1973;179:588–90.10.1126/science.179.4073.588Search in Google Scholar
22. Folhe L, Gunzler WA. Assays of glutathione peroxidase. Methods Enzymol 1984;105:114–21.10.1016/S0076-6879(84)05015-1Search in Google Scholar
23. Carlberg I, Mannervik B. Purification by affinity chromatography of yeast glutathione reductase, the enzyme responsible for the NADPH-dependent reduction of the mixed disulfide of coenzyme A and glutathione. Biochim Biophys Acta 1977;484:268–74.10.1016/0005-2744(77)90083-3Search in Google Scholar
24. Gosselin RE, Hodge HC, Smith RP, Gleason MN. Clinical toxicology of commercial products, 4th ed. Baltimore: Williams and Wilkins, 1976:II–168.Search in Google Scholar
25. Carroll JF, Dwyer TM, Grady AW, Reinhart GA, Montani JP, Cockrell K, et al. Hypertension, cardiac hypertrophy, and neurohumoral activity in a new animal model of obesity. Am J Physiol 1996;271:373–8.10.1152/ajpheart.1996.271.1.H373Search in Google Scholar PubMed
26. Bray GA, Paeratakul S, Popkin BM. Dietary fat and obesity: a review of animal, clinical and epidemiological studies. Physiol Behav 2004;83:549–55.10.1016/j.physbeh.2004.08.039Search in Google Scholar PubMed
27. Lutsey PL, Steffen LM, Stevens J. Dietary intake and the development of the metabolic syndrome; the atherosclerosis risk in communities study. Circulation 2008;117:754–61.10.1161/CIRCULATIONAHA.107.716159Search in Google Scholar PubMed
28. Hasty AH, Shimano H, Osuga J, Namatame I, Takahashi A, Yahagi N, et al. Severe hypercholesterolemia, hypertriglyceridemia, and atherosclerosis in mice lacking both leptin and the low density lipoprotein receptor. J Biol Chem 2001;276:37402–8.10.1074/jbc.M010176200Search in Google Scholar
29. Despres JP, Lemieux I, Bergeron J, Pibarot P, Mathieu P, Larose E, et al. Abdominal obesity and the metabolic syndrome: contribution to global cardio metabolic risk. Arterioscler Thromb Vasc Biol 2008;28:1039–49.10.1161/ATVBAHA.107.159228Search in Google Scholar
30. Satoh N, Wada H, Ono K, Yamakage H, Yamada K, Nakano T, et al. Small dense LDL-cholesterol relative to LDL-cholesterol is a strong independent determinant of hypoadiponectinemia in metabolic syndrome. Circ J 2008;72:932–9.10.1253/circj.72.932Search in Google Scholar
31. Fitch ME, Mangels AR, Altmann WA, El Hawry M, Qureshi AA, Elson CE. Microbiological screening of mevalonatesupressive minor plant constituents. J Agric Food Chem 1989;37:687–91.10.1021/jf00087a024Search in Google Scholar
32. Voors AA, Oosterga M, Buikema H, May JF, Grandjean JG, van Buiten A, et al. Dyslipidemia and endothelium-dependent relaxation in internal mammary arteries used for coronary bypass surgery. Cardiovasc Res 1977;34:568–74.10.1016/S0008-6363(97)00081-3Search in Google Scholar
33. Park JB, Touyz RM, Chen X, Schiffrin EL. Chronic treatment with a superoxide dismutase mimetic prevents vascular remodeling and progression of hypertension in salt- loaded strokeprone spontaneously hypertensive rats. Am J Hypertens 2002;15:78–84.10.1016/S0895-7061(01)02233-6Search in Google Scholar
34. Vaziri ND, Dicus M, Ho ND, Boroujerdi-Rad L, Sindhu RK. Oxidative stress and dysregulation of superoxide dismutase and NADPH oxidase in renal insufficiency. Kidney Int 2003;63:179–85.10.1046/j.1523-1755.2003.00702.xSearch in Google Scholar PubMed
35. Delles C, Miller WH, Dominiczak AF. Targeting reactive oxygen species in hypertension. Antioxid Redox Sign 2008;10:1061–77.10.1089/ars.2007.2008Search in Google Scholar PubMed
36. Tsukahara H, Hiraoka M, Kobata R, Hata I, Ohshima Y, Jiang MZ, et al. Increased oxidative stress in rats with chronic nitric oxide depletion: measurement of urinary 8-hydroxy-2-deoxyguanosine excretion. Redox Rep 2000;5:23–8.10.1179/rer.2000.5.1.23Search in Google Scholar PubMed
37. Rouanet JM, Decorde K, Del Rio D, Auger C, Borges G, Cristol JP, et al. Berry juices, teas, antioxidants and the prevention of atherosclerosis in hamsters. Food Chem 2010;1182:66–71.10.1016/j.foodchem.2009.04.116Search in Google Scholar
38. Vijayakumar RS, Nalini N. Efficacy of piperine, an alkaloidal constituent from Piper nigrum on erythrocyte antioxidant status in high fat diet and antithyroid drug induced hyperlipidemic rats. Cell Biochem Funct 2006;24:491–8.10.1002/cbf.1331Search in Google Scholar
39. Choi HS, Song HS, Ukeda H, Sawamura M. Radical-scavenging activities of citrus essential oils and their components: detection using 1,1-diphenyl-2- picrylhydrazyl. J Agric Food Chem 2000;48:41–56.10.1021/jf000227dSearch in Google Scholar
40. Woollett LA, Spady DK, Dietschy JM. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor and production rate. J Lipid Res 1922;33:77–88.10.1016/S0022-2275(20)41885-1Search in Google Scholar
41. Slim RM, Toborek M, Watkins BA, Boissonneault GA, Hennig B. Susceptibility to hepatic oxidative stress in rabbits fed different animal and plant fats. J Am Coll Nutr 2006;15:289–94.10.1080/07315724.1996.10718600Search in Google Scholar
42. Farooqui A, Ong WY, Horrocks LA. Neurochemical aspects of excitotoxicity. New York: Springer, 2008.Search in Google Scholar
43. Kedziora-kornatowska K, Szram S, Kornatowski T, Szadujkis-Szadurski L, Kedziora K, Bartosz G. Effect of vitamin E and vitamin C supplementation of antioxidative state and renal glomerular basement membrane thickness in diabetic kidney. Exp Nephrol 2003;95:134–43.10.1159/000074840Search in Google Scholar
44. Kirkman HN, Gaetani GF. Mammalian catalase: a venerable enzyme with new mysteries. Trends Biochem Sci 2007;3:44–50.10.1016/j.tibs.2006.11.003Search in Google Scholar
45. Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med 1995;27:951–65.10.1016/S0891-5849(99)00173-2Search in Google Scholar
46. Seema Farhath MS, Vijaya PP, Vimal M. Antioxidant activity of geraniol, geranial acetate, gingerol and eugenol. Res Pharm 2013;3:1–6.Search in Google Scholar
47. Vijayakumar RS, Surya D, Nalini N. Antioxidant efficiency of black pepper and piperine in rats with high fat diet induced oxidative stress. Redox Rep 2004;9:105–10.10.1179/135100004225004742Search in Google Scholar PubMed
48. Zhang H, Liu H, Davies KJ, Sioutas C, Finch CE, Morgan TE, et al. Nrf2-regulated phase II enzymes are induced by chronic ambient nanoparticle exposure in young mice with age-related impairments. Free Radic Biol Med 2012;2:2038–46.10.1016/j.freeradbiomed.2012.02.042Search in Google Scholar PubMed PubMed Central
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