Molecular Characterization of a Novel Mammalian
DnaJ-Like Sec63p Homolog

Markus H. Skowronek; Max Rotter; Ingrid G. Haas

doi:10.1515/BC.1999.142

Published by De Gruyter June 1, 2005

Molecular Characterization of a Novel Mammalian DnaJ-Like Sec63p Homolog

Markus H. Skowronek , Max Rotter and Ingrid G. Haas

From the journal Biological Chemistry

https://doi.org/10.1515/BC.1999.142

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Abstract

We identified a human cDNA sequence encoding a polypeptide of 760 amino acids that shares 53% homology and 25.6% identity with the yeast DnaJ-like endoplasmic reticulum (ER) translocon component Sec63p. Three epitope-specific antisera revealed a protein of an apparent molecular mass of 83 kDa, both in human cell extracts and in dog pancreatic microsomes. Biochemical analyses show that it is an integral membrane protein of the rough ER, which has the DnaJ domain located in the ER lumen. The novel Sec63 protein could thus represent a key component of the mammalian ER protein translocation machinery.

Published Online: 2005-06-01

Published in Print: 1999-09-13

Molecular Characterization of a Novel Mammalian DnaJ-Like Sec63p Homolog

Abstract

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