Electrophoresis Fundamentals: Essential Theory and Practice

Budin Michov

Published by De Gruyter 2022

Electrophoresis Fundamentals

Essential Theory and Practice

Budin Michov

In the series De Gruyter Textbook

https://doi.org/10.1515/9783110761641

Overview
Contents

About this book

The electrophoresis techniques are used in medicine, biochemistry, analytical chemistry, and biology to separate soluble and insoluble proteins, nucleic acids, chromosomes, viruses, as well as lysosomes, mitochondria, ribosomes and other cell organelles, red cells, tissue cells, and parasites. This book provides a view over the old electrophoresis techniques, as well as the recent developments in electrophoresis.

Electrophoresis Fundamentals is based on the recent book Electrophoresis: Theory and Practice published in 2020 by De Gruyter. The previous book combines theory and technical applications with troubleshooting and problem solving. While Electrophoresis is intended for specialists, Electrophoresis Fundamentals is a book for laboratory technicians, students, biochemists, general practitioners, and more.

Discusses modern electrophoresis methods such as the PCR technique, which is used to detect COVID-19.
Contains troubleshooting and problem solving.
Intended for students, chemists, biochemists, geneticists, physicians, and more.

Author / Editor information

Budin Michov, Sofia University, Sofia, Bulgaria.

Topics

Frontmatter Publicly Available Download PDF	I
Preface Publicly Available Download PDF	VII
About the Author Publicly Available Download PDF	IX
Contents Publicly Available Download PDF	XI
Abbreviations Publicly Available Download PDF	XXIX
1 Fundamentals of electrophoresis Requires Authentication Unlicensed Licensed Download PDF	1
1.1 Electric double layer of a charged particle Requires Authentication Unlicensed Licensed Download PDF	5
1.2 Proteins and nucleic acids form polyions in solution Requires Authentication Unlicensed Licensed Download PDF	13
1.3 Electrophoresis is running in buffers Requires Authentication Unlicensed Licensed Download PDF	19
1.4 Polyions are moving in electric field Requires Authentication Unlicensed Licensed Download PDF	27
1.5 Electrophoresis is carried out in different solid media Requires Authentication Unlicensed Licensed Download PDF	39
1.6 General theory of electrophoresis Requires Authentication Unlicensed Licensed Download PDF	49
1.7 Electrophoresis instrumentation Requires Authentication Unlicensed Licensed Download PDF	55
1.8 Classification of electrophoretic methods Requires Authentication Unlicensed Licensed Download PDF	61
2 Electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	71
2.1 Cellulose acetate electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	73
2.2 Agarose gel electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	77
2.3 Immunoelectrophoresis Requires Authentication Unlicensed Licensed Download PDF	109
2.4 Affinity electrophoresis Requires Authentication Unlicensed Licensed Download PDF	119
2.5 Polyacrylamide gel zone electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	131
2.6 Isotachophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	147
2.7 Disc-electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	151
2.8 Isoelectric focusing of proteins Requires Authentication Unlicensed Licensed Download PDF	187
2.9 Free-flow electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	209
2.10 Capillary electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	217
2.11 Two-dimensional electrophoresis Requires Authentication Unlicensed Licensed Download PDF	231
2.12 Preparative electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	241
2.13 Microchip electrophoresis of proteins Requires Authentication Unlicensed Licensed Download PDF	253
2.14 Blotting of proteins Requires Authentication Unlicensed Licensed Download PDF	271
2.15 Evaluation of protein pherograms Requires Authentication Unlicensed Licensed Download PDF	289
2.16 Precast gels for protein electrophoresis. Rehydratable gels Requires Authentication Unlicensed Licensed Download PDF	309
3 Electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	317
3.1 Agarose gel electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	325
3.2 Pulsed-field gel electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	343
3.3 Capillary electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	349
3.4 Polyacrylamide gel electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	357
3.5 Microchip electrophoresis of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	371
3.6 Blotting of nucleic acids Requires Authentication Unlicensed Licensed Download PDF	375
3.7 Evaluation of nucleic acid pherograms Requires Authentication Unlicensed Licensed Download PDF	385
3.8 Precast gels for nucleic acid electrophoresis Requires Authentication Unlicensed Licensed Download PDF	395
4 Iontophoresis Requires Authentication Unlicensed Licensed Download PDF	397
5 History of electrophoresis and iontophoresis Requires Authentication Unlicensed Licensed Download PDF	405
6 Troubleshooting Requires Authentication Unlicensed Licensed Download PDF	421
Problems Requires Authentication Unlicensed Licensed Download PDF	435
Solution of problems Requires Authentication Unlicensed Licensed Download PDF	441
Reagents for electrophoresis Requires Authentication Unlicensed Licensed Download PDF	447
Recipes for electrophoresis solutions Requires Authentication Unlicensed Licensed Download PDF	459
SI units and physical constants used in electrophoresis Requires Authentication Unlicensed Licensed Download PDF	475
Electrophoresis terms Requires Authentication Unlicensed Licensed Download PDF	481
Index Requires Authentication Unlicensed Licensed Download PDF	485

(Deutsch)

Language: English
Publisher: De Gruyter
Audience: Graduate students in chemistry, biochemistry, molecular biology and laboratory medicine.
Pages
- Front matter: 31
- Main content: 490
Illustrations
- Illustrations: 12
- Coloured Illustrations: 149
- Tables: 50
Keywords: Electrophoresis; Biochemistry; Chemistry; Analytical Chemistry; Laboratory Medicine.

eBook

Published: April 4, 2022
ISBN: 9783110761641

Paperback

Published: April 18, 2022
ISBN: 9783110761627