A salivary gland chromosome map of Anopheles (Cellia) fluviatilis James is produced. This has been compared with those of Anopheles stephensi stephensi and Anopheles pulcherrimus. The X-chromosome similarities are the least, whereas 2R homologies among them are the commonest. The free ends are fairly uniform in Anopheles fluviatilis and Anopheles pulcherrimus. Anopheles fluviatilis shares a fifty-fifty relationship with pulcherrimus and stephensi.