Cytological observations on 17 W. Himalayan taxa belonging to Smilacaceae, Liliaceae and Trilliaceae are presented. Four species have been investigated for the first time. These are Smilax elegans (n=16), S. parvifolia (n=16), Lilium polyphyllum (2n=24) and Gagea persica (n=24). New chromosome number has been recorded in Gagea lutea (n=48). Detailed karyotype analysis has been made in Smilacina purpurea (2n=36), Eremurus himalaicus (n=7), Lilium cordifolium (2n=24), L. polyphyllum (2n=24), Fritillaria roylei (2n=24), Kniphofia uvaria (2n=12), Trillium govanianum (2n=20) and Paris polyphylla (2n=20).