Cytological studies have been made of 12 species of tribe Senecioneae (Asteraceae) from north and central India. Of these, 3 species, namely S. grahami Hook. f. (n=10), S. kunthianus Wall ex DC. (n=20) and S. sexatilis Sensu Hook. f. (n=10), are counted for the first time. Additional and/or variable cytotypes are recorded for 2 species, namely S. nudicaulis Buch.-Ham D. Don. (n=5, 10) and S. rufinervis DC. (n=10). Existence of B-chromosomes has been recorded in S. krascheninnikovii Schischk. (n=10+0−2B). Two species, S. vulgaris L. (n=20) and S. krascheninnikovii Schischk. (n=10), are explored for the first time from India. Besides these, cytomorphological variabilities have been observed in S. rufinervis (2n=20, 40) and S. nudicaulis Buch-Ham ex D. Don (2n=10, 20 and 40). Great variation in morphological characteristic have been noticed in different accessions of S. laetus Edgew, however all the accessions are found to exist at 2n=40. Meiotic configurations in the form of multivalents and/or secondary association of bivalents are found in Gynura nepalensis DC., Senecio laetus Edgew, S. kunthianus Wall ex DC., S. krascheninnikovii Schischk. and S. nudicaulis Buch-Ham ex D. Don.