Endocrinologia Japonica
Online ISSN : 2185-6370
Print ISSN : 0013-7219
ISSN-L : 0013-7219
HISTOCHEMISCHE UNTERSUCHUNGEN ÜBER DIE ENDOMETRIALE CARBOANHYDRASE DES KANINCHENS
GENJI NAGATATAMOTSU MIYAKE
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1960 Volume 7 Issue 3 Pages 202-214

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Abstract

The biochemical and histochemical investigations presented in this paper confirm that progesterone administered into the estrogen-primed immature female rabbit produces marked increase of carbonic anhydrase in the endometrium.
It is clearly demonstrated that the increases of carbonic anhydrase induced by progesterone takes place in the glandular portion of uterine mucosa and the precipitation of manganese granules resulting from the enzyme reaction are concentrated in the epithelial cells.
Acetazoleamide administered intravenously 30 mins. before autopsy causes marked reduction of endometrial carbonic anhydrase activity and poor stainning of the uterine section prepared by the Hausler's (1958) cobalt sulfate method for carbonic anhydrase.
Comparing the enzyme activity of one horn perfused with saline before the enzyme extraction with that of the other horn without perfusion on the same uterus, the activities derived from the blood contaminated in the extracts are investigated for a series of control and progesterone-treated groups. The data demonstrate that the rate of enzyme activity resulted from blood contamination in the extract of progesterone-treated group is rather smaller than that in the control group.
It is, therefore, concluded that the increase of endometrial carbonic anhydrase in the Clauberg rabbit treated with progesterone is probably due to the new biosynthesis of the enzyme in the epithelial cells of uterine mucosa.

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© The Japan Endocrine Society
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