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Extraction of nucleic acids from Gram-positive bacteria is normally hampered by a thick and resistant cell wall. This paper presents procedures based on mechanical cell breakage to extract DNA from St...aphylococcus aureus. The proposed system for DNA extraction involves bead-beating treatment and the GES method. These two steps allow a consistent extraction from the bacterium resistant to the GES method alone. Yield and quality of DNA obtained with the proposed method were higher than those obtained with the GES method alone. This protocol can be extended to clinical specimens.show more
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shsjournal2004-1_04