Serum Renalase Level as A Marker of Activity and Severity in Lupus Nephritis Cases

Int. J. Clin. Rheumatol. (2021) 16(9), 249-254 ISSN 1758-4272 Introduction Renal disease is a severe manifestation of systemic lupus erythematosus (SLE), accounting for a significant share of death and morbidity [1]. Up to 90% of persons with SLE will have pathologic indications of kidney damage at the time of biopsy, even though only 50% will develop clinically serious nephritis [2]. Lupus nephritis manifests clinically in a number of different ways, from asymptomatic hematuria and/or proteinuria to a full-blown nephrotic syndrome to a rapidly increasing glomerulonephritis with accelerating renal dysfunction. Although there are exceptions, lupus nephritis normally improves within the first 36 months of the disorder. Thus, screening for nephritis on a monthly basis is crucial for the continuous evaluation and therapy of cases with SLE [3].


Introduction
Renal disease is a severe manifestation of systemic lupus erythematosus (SLE), accounting for a significant share of death and morbidity [1]. Up to 90% of persons with SLE will have pathologic indications of kidney damage at the time of biopsy, even though only 50% will develop clinically serious nephritis [2]. Lupus nephritis manifests clinically in a number of different ways, from asymptomatic hematuria and/or proteinuria to a full-blown nephrotic syndrome to a rapidly increasing glomerulonephritis with accelerating renal dysfunction. Although there are exceptions, lupus nephritis normally improves within the first 36 months of the disorder. Thus, screening for nephritis on a monthly basis is crucial for the continuous evaluation and therapy of cases with SLE [3].
can be secreted into the bloodstream by the kidneys [5]. Renalase has previously been shown to assist in regulating blood pressure by degrading catecholamines in the bloodstream [6]. Renalase treatment is associated with renal protection and decreased macrophage infiltration in a mouse model of acute kidney injury (AKI), showing that renalase has an anti-inflammatory role in renal dysfunction [7,8].
The association between renalase and inflammation has been demonstrated in investigations of organ transplantation and serum renalase concentrations in kidney and heart cases [9,10]. The purpose of this study was to examine whether serum renalase levels were associated with renal pathology and disease activity in lupus nephritis, to hypothesize a function for renalase in this autoimmune and inflammatory disorder, and to assess whether it is an excellent biomarker for lupus nephritis.

Method
This study included 48 patients with SLE who were evaluated through using Systemic Lupus International Collaborating Clinics (SLICC) (2015) [11] or the New EULAR/ACR SLE Classification Criteria 2017 [12] and 30 healthy controls. They were gathered from the rheumatology and rehabilitation departments of the AL-Azhar University hospitals' inpatient and outpatient clinics. The ethical council of Al-Azhar Medical School approved this study, and all subjects were given written informed permission before participation.
All individuals with life-threatening conditions other than LN were eliminated from this investigation (e.g., heart failure, central nervous system lupus, malignant tumor, infectious disease), as well as those with an eGFR of less than 30ml/min/1.73 m2 or who was pregnant at the age of 18 or 50.
On the basis of SLEDAI (SLE Disease Activity Index) ratings, the recruited cases were split into two equal groups [13]: Group II included 23 patients of lupus with disease activity as determined by the SLEDAI.
Group III had 23 lupus patients with no signs of disease activity, as determined by the SLEDAI.
The control group (Group I) consists of 23 persons who appear to be in good health and are of comparable age and sex.
The following items were covered in a detailed examination of all participants: Complete history taking, investigations of the general and local areas; Upon that, during the study visit, venous blood samples were obtained by all participants in the prescribed sequence: C-reactive protein (CRP), complete blood counting, C-reactive protein (CRP), sedimentation rate of erythrocytes (ESR), liver-function testing, creatinine serum, uric blood urea, and serum, and urine proteins 24hour. To assess urinary protein excretion, 24-hour urine and spot urine samples were obtained. Autoimmune Profile: C3 and DNA-Anti-double-strand (anti-ds-DNA-Ab) complements were evaluated by the use of an enzyme-related immune-sorbent assay (ELISA). Renal biopsies were percutaneously collected from LN cases involved with this study using an ultrasonographically guided biology or computed tomography needle, and paraformaldehyde-fixed air-dry slices of the frozen LN kidney sample were delivered to the histopathologist. Renal samples were categorized in accordance with the International Society of Nephrology/Renal Pathology (ISN/RPS) [14].
At the study visit, serum renalase was taken, and serum was separated within three hours of collection. To avoid repeated freeze cycles, serum was separated using Rotofix32 (Hettich-zentrifugen) at 2000x for 20 minutes and then gathered into at least four aliquots and kept at -20°C until required for analysis. Serum renalase concentrations were determined as per the manufacturer's procedure by an ELISA kit specific to renalase. The concentration of human serum Renalase (RNLS) was established by a highly sensitive, commercial sandwich enzyme immunoassay employing an (RNLS) serum renalase. This assay is very specific and sensitive for detecting (RNLS). There was no evidence of considerable cross-reactivity or interaction between (RNLS) and analogs [15].
The 2000 SLE Illness Activity Index (SLEDAI-2K) and Renal SLEDAI (rSLEDAI) have been used for evaluating the activity of the disease and renal disease consecutively [13]. RSLEDAI includes haematuria, pyuria, proteinuria, and urine casts (SLEDAI-2K renal scores). Cases with LN were divided into two categories according to their SLEDAI scores, the active LN (SLEDAI <8) and the quiescent LN (SLEDAI <8).

Statistical analysis
The Social Science Statistics Program (SPSS) version 20.0 was used to examine the data. The standard difference and average of quantitative data were calculated (SD). Frequency and percentage of qualitative data were used. The following tests have been performed: The independent samples t-test of significance was used Short Communication Elmotaleb Elsayed, et al.

Short Communication
Serum Renalase Level as A Marker of Activity and Severity in Lupus Nephritis Cases when comparing two means. A one-way variance analysis (ANOVA) is utilized when comparing more than two means. The Chi-square (X2) meaning test was used to examine the proportions between two qualitative parameters. Relationships were created through Pearson's coefficient of relationship (r) test. The trust interval was set at 95%, while the acceptable error margin was set at 5%. Therefore, the following p-value was judged significant: The likelihood (P-value) P-value less than 0.05 was considered important. A P-value less than 0.001 was considered to be very important. P-value >0.05 was considered negligible.

Results
Around 46 LN patients and 23 healthy controls were part of the current study. The LN group comprised five male and forty-one female cases with a mean age of 30.1±9.196. Table 1 outlines the participants' demographic information. Significant changes have been seen between healthy controls and LN cases in the systolic blood pressure (P=0,017).
Also, Table 2 presented the comparison between cases' groups as per Renal biopsy Class where Class III was predominant in both active lupus nephritis group 13 cases (56.5%) and inactive LN group 12 cases (52.2%) In LN, the serum renalase level was substantially higher in our research objective (132,35±92,59 vs. 30,26±17,67 μg/ml, P-value less than0,001) ( Table 3).  The blood renalase levels of patients with Active LN (group II) were strongly linked with CRP but not with the nephritis class. Figures 1 and 2) However, instances with SLE with inactive LN (group III) did not show a statistically significant correlation between their serum renalase and any of the clinical or laboratory markers. However, they did show a negative correlation with the nephritis class, as shown in (Table 4).
Additionally, there was no significant association between markers of renal activity and serum renalase concentrations in subjects with active LN, such as proteinuria, ESR, anti-dsDNA, C4, and C3 ( Table 5).

Discussion
Without question, early diagnosis of LN is crucial for persons with SLE to be treated effectively [16]. LN is a well-established risk factor for mortality and morbidity in SLE, and even when immunosuppressive therapies are employed, many patients improve from end-stage renal disease (ESRD) or chronic kidney disease (CKD) [17].
Biomarkers predictive of the development of active LN would be tremendously beneficial, as early diagnosis and treatment could improve renal outcomes. Antibodies against dsDNA complement C3 and C4 were previously used to monitor disease activity in LN. Nonetheless, these serological indicators of autoimmunity are insufficiently highly sensitive and specific to be used as a biomarker for LN activity [18].
Renalase (monoamine oxidase) has been implicated in the pathogenesis of LN and its flare; consequently, we sought to investigate the serum level of renalase in SLE patients with active or inactive LN.
This cross-sectional study included approximately 48 cases of SLE and nephritis, 23 active cases, 23 inactive cases, and 30 healthy controls.
In our current investigation, we discovered that LN cases had considerably greater concentrations of renalase than healthy controls (P-value less than0.001).
Additionally, those with active LN had significantly higher serum renalase levels than those with inactive LN (P-value less than 0.005), indicating that renalase levels may be related to disease severity in LN, especially the proliferating type.
We approved the link between clinical activity and serum renalase in LN cases using relation analysis.
Additionally, serum renalase levels were found to be significantly linked with rSLEDAI (r2 = 0.37, P-value less than 0.001) and 24-hour urine protein excretion (r2 = 0.417, P = 0.001). Serum Renalase expression was not detected in the glomeruli under normal circumstances. Simultaneously, patients with proliferative LN had significantly greater serum renalase concentrations than those with Class V LN [19].
As revealed in a 2005 study by Xu J, Li G, et al., renalase is involved in the control of cardiovascular function and blood pressure. (Renalase is a novel soluble monoamine oxidase that affects heart function and blood pressure) [5], and Desir GV et al. 2012 .'s study. (Renalase is responsible for ambulatory blood pressure decrease by metabolizing circulating adrenaline [20]. Other several research, on the other hand, revealed no correlation between blood pressure and serum renalase in patients receiving hemodialysis or peritoneal dialysis [21,22]. Blood pressure may continue to have an effect on serum renalase. Nevertheless, because blood pressure varied little between groups, it is possible that blood pressure had a slight impact on our investigation. Recent investigations demonstrated that renalase effectively ameliorated renal injury caused by cisplatin and hydrogen peroxide attacks on human proximal tubular (HK-2) cells. The renalase's administration is related to decreased macrophage infiltration and renal protection in the acute kidney injury (AKI) mice model. In contrast, macrophage infiltration and renal injury are more severe in the renalase knockout mice model, implying that renalase plays an anti-inflammatory role in kidney injury [7,8].
The investigations on organ transplantation and blood renalase concentrations discovered increased renalase concentrations in the heart and kidney recipients, implying a link between inflammation and renalase [9,10].
Several intriguing studies established a link between renalase and type 1 diabetes, implying that it may also have a role in developing autoimmune pancreatic damage [23][24][25].
The association of renalase with the development of organ transplant recipients and autoimmune diabetes shows that it has a role in the pathophysiology of immune-mediated illnesses. Even though data from the research outlined above are available, renalase's expression and clinical importance in individuals with LN remain unknown.
The study was cross-sectional and single-center, and a prospective, multi-center experiment will be done to explain further and validate the findings. The future study will evaluate the time point at which renalase levels increase in response to LN, the differences in serum renalase levels across different types of kidney disease, and if renalase is involved in the mechanism or etiology of LN.

Conclusion
Serum renalase was related to disease activity in LN, especially the proliferative form, implying that it may be employed as a biomarker in prospective clinical studies. Further research is necessary to determine the efficiency of serum renalase as a biomarker of disease severity in lupus nephritis.