Optimal human ovarian follicle isolation: A review focused on enzymatic digestion
Farnaz Tajbakhsh1,2, Somayeh Tavana1, Mohammad Kazemi Ashtiani3, Christiani Andrade Amorim4 and Rouhollah Fathi1
1Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, 2Department of Developmental Biology, University of Science and Culture, 3Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran and 4Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium
Corresponding Author: Rouhollah Fathi, Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. e-mail: rfathi79@royaninstitute.org
Summary. The damage or depletion of ovarian reserves due to aging or cancer treatment can increase the need for fertility preservation techniques. One of the most common ways of supporting fertility in prepubertal girls and women who require immediate cancer treatment is through ovarian tissue cryopreservation and re-transplantation following cancer treatment. However, a more appropriate method should be employed in diseases such as leukemia, where malignant cells may be present in cryopreserved tissue, instead of ovarian tissue transplantation. Human ovarian follicle isolation for in vitro culture or the use of artificial ovaries for their growth can decrease the risk of reintroducing cancer cells into these individuals. Here we review the methods for the isolation of human ovarian follicles. Histol Histopathol
Key words: Human ovarian tissue, Follicle isolation, Enzymatic isolation, Artificial ovary, Live/dead
DOI: 10.14670/HH-18-747
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©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License. |
