Immunohistochemical evaluation of tissues following bone implant extraction from upper and lower limb
Hana Bielniková-Kryštofová1,2,3, Oldřich Motyka4,5, Vladimír Židlík1,3, Dušan Žiak1,3, Iveta Szotkovská1,3, Jozef Škarda1,2,6, Jiří Voves1,7, Jana Pometlová1,7, Leopold Pleva1,7, Miroslav Havlíček8 and Kristina Čabanová2,4
1Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, 2Centre for Advanced Innovation Technologies, VŠB - Technical University of Ostrava, 3Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava and University of Ostrava, 4Faculty of Mining and Geology, VŠB - Technical University of Ostrava, 5Nanotechnology Centre, CEET, VŠB - Technical University of Ostrava, 6Laboratory of Molecular Pathology, Department of Clinical and Molecular Pathology and Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital Olomouc, Olomouc, 7Department of Trauma Surgery, University Hospital Ostrava, Ostrava and 8Medin, a.s., Nové Město na Moravě, Czech Republic
Corresponding Author: Hana Bielniková-Kryštofová, Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava, 17. listopadu 1790/5, 70852, Ostrava, Czech Republic. e-mail: hana.krystofova@fno.cz
Summary. Fractured bones can regenerate and restore their biological and mechanical properties to the state prior to the damage. In some cases, however, the treatment of fractures requires the use of supportive implants. For bone healing, three processes are essential: the inflammatory phase, the repair phase and the remodelling phase. A proper course of the first - inflammatory - stage is important to ensure a successful fracture healing process. In our study, we evaluated tissue samples immunohistochemically from the area surrounding the fractures of upper and lower limbs (bone tissue, soft tissue, and the implant-adhering tissue) for markers: CD11b, CD15, CD34, CD44, CD68, Cathepsin K, and TRAcP that are linked to the aforementioned phases. In soft tissue, higher expressions of CD68, CD34, CD15 and CD11b markers were observed than in other locations. TRAcP and Cathepsin K markers were more expressed in the bone tissue, while pigmentation, necrosis and calcification were more observed in the implant-adhering tissue. Since even the implant materials commonly perceived as inert elicit the observed inflammatory responses, new surface treatments and materials need to be developed. Histol Histopathol 38, 1119-1127 (2023)
Key words: Fracture, Inflammation, Healing, Foreign body reaction, Pigmentation
DOI: 10.14670/HH-18-606
 |
©The Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License. |
