Long non-coding RNA SNHG7 promotes malignant melanoma progression through negative modulation of miR-9

Wendi Wang, Guangjing Liu, Man Liu and Xiaobing Li

Department of Plastic and Burn Surgery, Tianjin First Center Hospital, Tianjin, China

Offprint requests to: Xiaobing Li, Department of Plastic and Burn Surgery, Tianjin First Center Hospital, No.24 Fukang Road, Nankai District, Tianjin 300192, China. e-mail: lixiaobing_tfch@163.com

Summary. Long non-coding small nucleolar RNA host gene 7 (lncRNA SNHG7) was verified to act as an onco-gene in human cancers. Nevertheless, the role of SNHG7 in malignant melanoma remains elusive. The present study showed an increase of SNHG7 expression in malignant melanoma tissues and cell lines. Besides, SNHG7 knockdown inhibited proliferation and migration in malignant melanoma cells. Bioinformatics analysis demonstrated that SNHG7 functions as a molecular sponge for miR-9 in biological behavior of melanoma cells. And miR-9 could inhibit the expression of PI3KR3 by binding with the 3' -UTR. Furthermore, PI3KR3, pAKT, cyclin D1 and Girdin expression was down-regulated after SNHG7 knockdown by siRNA. In addition, SNHG7 knockdown decreased xenograft growth in vivo. Taken together, this research demonstrated that SNHG7 was an oncogene in malignant melanoma, providing a novel insight for the pathogenesis and new potential therapeutic target for malignant melanoma. Histol Histopathol 35, 973-981 (2020)

Key words: Malignant melanoma, SNHG7, miR-9, PI3K/AKT signaling pathway

DOI: 10.14670/HH-18-225