KERAGAMAN BEBERAPA TUMBUHAN CIPLUKAN (Physalis spp.) DI LERENG GUNUNG KELUD, JAWA TIMUR
Nugraheni Hadiyanti, Supriyadi Supriyadi, Pardono Pardono
| Abstract views: 4786
| 
PDF views: 26698
Abstract
Ciplukan (Physalis spp.) is a wild plant which is that potentially used as a medicinal plant. Information related to the diversity of Physalis spp. in Indonesia is limited. The objectives of the research was to identify the diversity of wild Physalis sp. on Mt. Kelud, East Java based on morphological characters, total protein profiles, and flavonoid compounds. The research was conducted by survey on some gradients viz. 200–400, 400–600, 600–800, 800–1,000 and >1,000 m above sea level. Identification of morphological characters was focused on root, stem, leaf, flower and fruit covering 16 characters for scoring. Identification of total protein profile was performed by SDS-PAGE method and the flavonoid compound was analyzed using UV spectrophotometric method based on the colorimetric principle. The degree of diversity of Physalis spp. was determined based on morphological similarity and total protein profiles through cluster analysis. The results showed that Physalis spp. on Kelud was found up to 1,000 m above sea level. Altitude affected to the morphological diversity, total protein profile and the flavonoid compound. Morphological features that play a role in the diversity of Physalis sp. were the direction of growing stems, leaf shape, leaf margin, leaf surface, leaf stalk color, stain color on the inner neck of thr flower, and fruiting calyx color. Expression of total protein P. minima. shows similarity to P. angulata L. Physalis sp. in the low gradient have a higher flavonoid compound as compared to the middle and high gradients. There were two species of Physalis found on the gradient of Mt. Kelud, East Java, namely Physalis minima L. and Physalis angulata L.
Keywords
Full Text:
PDFReferences
Astuti, P., Supriyadi dan Supriyono., 2012. Karakterisasi fenotipe kultivar padi tahan dan rentan wereng coklat, Nilaparvata lugens Stal. (Hemiptera: Delphacidae). Indonesian Journal of Entomology, 9(2), pp. 57–63.
Akerele, O. 1992. World Health Organization guidelines for the assessment of herbal medicine. Fitoterapia, 62, pp. 99–110.
Bertoncelli, D.J., Marisa, C.B., Cristiane, O.B., Amanda, I.P., Ana, C.A. and Alexandre, O.O., 2016. Chemical characteristics of fruits of two spesies of Physalis under nitrogen fertilization. African Journal of Agricultural Research, 11(20), pp.1872–1878.
Burkill, H.M., 2000. The Useful Plants of West Tropical Africa. Royal Botanic Gardens, Kew. 5 (Edition 2). Claudia, Oliveira, M.C., Loss Edenes, M.S., Gomes, I., Pacheco, V. and Negri, R.C., 2016. Mineral and organic fertilizer in two Physalis spesies. African Journal of Agriculture, 12(2), pp. 104–110.
Chaves, A.C., 2006. Propagação e Avaliação Fenológica de Physalis sp. Naregião de Pelotas, 65 f. Tese (Doutorado em Ciências-Fruticultura de Clima Temperado) Universidade Federal de Pelotas, Pelotas, RS.
Chang, C.M., Yang, H., Wen and Chern, J., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), pp.178–182.
Coats, S.A., Wicker, L. and McCoy, C.W., 1990. Protein variation among fuller rose beetle population from Florida, California, and Arizona (Coleoptera: Curculionidae). Annuals Entomology Society of America, 83, pp.1054–1062.
El Sheikha, A.F., Piombo, G., Goli, T. and Montet, D., 2010. Main composition of Physalis (Physalis pubescens L.) fruit juice from Egypt. Fruits, 65(4), pp. 255–265.
Jones, S.B., and Luchsinger, A.E. 1987. Plant Systematics 2nd ed. McGraw-Hill. Inc., New York. 512 Ferita, I., Jamsari., Suliansyah, I., Gustian. Studi Hubungan Karakter Morfologi, Anatomi, Molekuler Terkait Potensi Kadar Katekin pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb). Disertasi. Fakultas Pertanian. Universitas Andalas. Padang.
Korner, C. 2007. The use of atitude in ecological research. Trends in Ecology and Evolution, 22 (11), pp. 569–574.
Laily, A.N., Suranto dan Sugiharto. 2012. Karakterisasi Carica pubescens di Dataran Tinggi Dieng, Jawa Tengah berdasarkan sifat morfologi, kapasitas antioksidan dan pola pita protein. Nusantara Bioscience, 4, pp. 16–21.
Lailani, K.N., 2008. Analisis Keragaman Protein dan Fitokimia Tanaman Pegagan (Centella aciatica) Hasil Perbanyakan in Vitro. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Pertanian Bogor. Bogor.
Larashati, I., 2004. Keanekaragaman tumbuhan dan populasinya di Gunung Kelud, Jawa Timur. Biodiversitas, pp. 71–76.
Ladizinsky, G. and Hymowitz, T., 1979. Seed protein electrophoresis in taxonomic and evolutionary studies. Theory Applied Genetic, 54, pp. 145–151.
McGarigal, K., Cushman, S., and Stafford, S. 2000. Multivariate Statistics for Wildlife and Ecology Research. Springer-Verlag. New York. 283.
Ncube B, Finnie JF and Van Staden J. 2012. Quality from the Field: The impact of environmental factors as quality determinants in medicinal plants. South African Journal of Botany, 82, pp. 11–20.
Olorode, O., Olayanju and Garba, S., 2013. Solanaceae in Nigeria. Ife Journal of Science, 15(1), pp. 101–109.
Pessarakli M, Haghighi M, and Sheibanirad A. 2015. Plant responses under environmental stress condition. Advances in Plants & Agriculture Research, 2(6), pp.00073.
Rahayu, S., Sumitro, S.B., Susilawati, T. dan Soemarno, 2006. Analisis isoenzim untuk mempelajari variasi genetik sapi Bali di Provinsi Bali. Hayati, 12, pp. 1–5.
Reed, S. 2008. Stakeholder participation for enviromental management. Literature Review. Journal Biological Conservation. 141. pp 2417–2431.
Suranto., 2002. Pengaruh lingkungan terhadap bentuk morfologi tumbuhan. Enviro, 1(2), pp. 37–40.
Steenis, C.G.G.J. van. 2008. Flora untuk Sekolah di Indonesia. Cetakan Kedua Belas. (diterjemahkan oleh Moeso Surjowinoto). PT. Pradnya Paramita. Jakarta.
Tomassini, T.C.B., Barbi, N.S., Ribeiro, I.M. and Xavier, D.C.D., 2000. Gênero Physalis – uma revisão sobre vitaesteróides. Quim. Nova, 23(1), pp. 4757.
Tjitrosoepomo, G., 1989. Morfologi Tumbuhan. Gadjah Mada University Press. Yogyakarta. Usaizan, N., Abdullah, N.A.P., Ahmad, S.H. and Saleh, G. , 2014. Preliminary phytochemical screening and GCMS analysis of ethanol extract of Physalis minima L. (Solanaceae). Journal of Advanced Agricultural Technologies, 1(2), pp. 100–103.
Viestra, R.D., 1993. Protein degradation in plants. Plant Physiology, 4, pp. 385–410. Wulandari, Retnaningtyas, Y., Nuri and Lukman, H., 2016. Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics. Journal of Analytical Methods in Chemistry, Article ID 4696803.
Wei, J., Hu, X., Yang, J., Yang, W., 2012. Identification of single-copy Orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers. Plos One, 7(11), pp. e50164.
Whitson, M., 2012. Calliphysalis (Solanaceae): a New Genus from Southeastern USA. Rhodora, 114 (958), pp. 133–147.
Wijoyo, P.M. 2008. Sehat dengan Tanaman Obat. Bee Media Indonesia. Jakarta.
Wigati. 2003. Variasi Genetik Ikan Anggoli (Pristipomoides multidens) berdasarkan Pola Pita Isozim. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Sebelas Maret. Surakarta.
Wongosupantio, S., 1992. Electroforesis Gel Protein. Universitas Gadjah Mada Press. Yogyakarta.
Refbacks
- There are currently no refbacks.