Open Access
Research (Published online: 04-05-2024)
16. Molecular detection of Klebsiella pneumoniae producing extended-spectrum beta-lactamase isolated from bat feces from the Tanjung Ringgit bat cave, Lombok Island, Indonesia
Kurnia Nisa Kinasih, Yolla Rona Mustika, Yulianna Puspitasari, Wiwiek Tyasningsih, Alfiana Laili Dwi Agustin, Shendy Canadya Kurniawan, Abdullah Hasib, Yusac Kristanto Khoda Waruwu, and Otto Sahat Martua Silaen
International Journal of One Health, 10(1): 133-140

Kurnia Nisa Kinasih: Master Program of Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia; Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Yolla Rona Mustika: Master Program of Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia; Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Yulianna Puspitasari: Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Wiwiek Tyasningsih: Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Alfiana Laili Dwi Agustin: Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Shendy Canadya Kurniawan: Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, Netherlands.
Abdullah Hasib: School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD, 4343, Queensland.
Yusac Kristanto Khoda Waruwu: Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
Otto Sahat Martua Silaen: Doctoral Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia.

doi: 10.14202/IJOH.2024.133-140

Article history: Received: 28-12-2023, Accepted: 20-03-2024, Published online: 04-05-2024

Corresponding author: Wiwiek Tyasningsih

E-mail: witya_kh@yahoo.com

Citation: Kinasih KN, Mustika YR, Puspitasari Y, Tyasningsih W, Agustin ALD, Kurniawan SC, Hasib A, Waruwu YKK, and Silaen OSM (2024) Molecular detection of Klebsiella pneumoniae producing extended-spectrum beta-lactamase isolated from bat feces from the Tanjung Ringgit bat cave, Lombok Island, Indonesia, Int. J. One Health, 10(1): 133-140.
Abstract

Background and Aim: Bats are a reservoir for the pathogenic bacteria Klebsiella pneumoniae and can spread it through feces that fall in nests/caves, carried, and dropped while they fly near human settlements, and from their saliva. The emergence and spread of multidrug resistance (MDR) strains of K. pneumoniae indicate that resistant to antibiotics, especially extended-spectrum beta-lactamase (ESBL), is considered an important global health threat. The aim of this study was to determine the presence of the gene encoding extended beta-lactamase in K. pneumoniae isolated from fresh bat feces collected from the Tanjung Ringgit bat cave, East Lombok.

Materials and Methods: In this study, 150 fresh fecal samples were analyzed using standard microbiological techniques for the presence of K. pneumoniae. K. pneumoniae-positive isolates were subjected to antibiotic sensitivity testing, followed by molecular detection using polymerase chain reaction.

Results: This study showed that 14 (9.3%) of 150 samples were positive for K. pneumoniae. Ten of the 14 samples (71.4%) were MDR isolates and 6 (42.9%) had the blaSHV gene identified.

Conclusion: The presence of K. pneumoniae isolated from fresh bat feces, which is MDR and has the blaSHV gene encoding ESBL indicates that bats can be a reservoir for the transmission of MDR and ESBL bacteria has an impact on public health in the study area.

Keywords: bat, blaSHV, extended-spectrum beta-lactamase, Klebsiella pneumoniae, public health.