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HLA antibody analysis

Sensitivity, specificity, and clinical significance in solid organ transplantation

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Abstract

The clinical relevance of humoral allosensitization has gained a lot of attention in the last few years. An increasing number of studies have demonstrated adverse graft survival in patients who have either preformed or post-transplant-developed anti-HLA antibodies. The detection of HLA antibodies and the specificity analysis have evolved over time from primarily cell-based to solid-phase methods, including the availability of single-HLA antigen preparations. These technological advances combined with a better understanding of the epitope structure of HLA antigens have provided a more efficient, structurally based strategy to determine HLA compatibility. In conclusion, these emerging approaches can be reliably used to predict crossmatch results in highly sensitized patients and also to monitor the development of clinically relevant anti-HLA antibody after transplantation.

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Author notes

  1. Adriana Zeevi PhD

    Present address: Biomedical science Tower, 200 Lothrop St. w1551, 15261, Pittsburgh, PA, USA

Authors and Affiliations

  1. Departments of Pathology Transplantation Institute, University of Pittsburgh, Pittsburgh, PA

    Adriana Zeevi PhD, Alin Girnita & Rene Duquesnoy

  2. Departments of Immunology, Thomas E Starzl, Transplantation Institute, University of Pittsburgh, Pittsburgh, PA

    Adriana Zeevi PhD & Rene Duquesnoy

Authors
  1. Adriana Zeevi PhD
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  2. Alin Girnita
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  3. Rene Duquesnoy
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Zeevi, A., Girnita, A. & Duquesnoy, R. HLA antibody analysis. Immunol Res 36, 255–264 (2006). https://doi.org/10.1385/IR:36:1:255

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