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Bioelectrocatalysts

Engineered oxidoreductase system for utilization of fumarate reductase in chemical synthesis, detection, and fuel cells

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Abstract

Fumarate reductase was used as a model oxidoreductase to demonstrate continuous electrical cofactor reduction-oxidation during the bioelectrochemical synthesis and detection of chemicals. The enzyme preparation was immobilized onto a graphite felt electrode that was modified with carboxymethylcellulose (CMC). Nicotinamide adenine dinucleotide (NAD), neutral red, and fumarate reductase (which contained menaquinone) were covalently linked by peptide bonds to the CMC. The electron mediator neutral red allowed NAD and menaquinone to be recycled electrically during enzymatic chemical synthesis. Succinate detection by the bioelectrocatalyst was linear from 5 μM to 10 mM succinate. Fumarate synthesis using this bioelectrode was dependent on succinate utilization and resulted in proportional production of electricity and fumarate. Succinate synthesis using this bioelectrocatalyst was dependent on current and fumarate concentration. This bioelectrocatalyst system may enhance the utility of menaquinone- and/or pyridine nucleotide-linked oxidoreductases in diverse enzymatic fuel cells and sensors. It may also enhance the utility of oxidoreductase-based chemical synthesis systems because it eliminates the problem of cofactor recycling.

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Author notes

  1. Doo Hyun Park

    Present address: Department of Biological Engineering, SeoKyeong University, 16-1 Jungneung-dong, Sungbuk-gu, 136-704, Seoul, Korea

Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Michigan State University, 48824-1319, East Lansing, MI

    Doo Hyun Park, C. Vieille & J. G. Zeikus

Authors
  1. Doo Hyun Park
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  2. C. Vieille
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  3. J. G. Zeikus
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Correspondence to J. G. Zeikus.

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Park, D.H., Vieille, C. & Zeikus, J.G. Bioelectrocatalysts. Appl Biochem Biotechnol 111, 41–53 (2003). https://doi.org/10.1385/ABAB:111:1:41

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  • DOI: https://doi.org/10.1385/ABAB:111:1:41

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