Skip to main content
SpringerLink
Log in

Bone marrow hyaluronan distribution in patients with acute myeloid leukemia

  • Original Article
  • Published:
Medical Oncology Aims and scope Submit manuscript

Abstract

Acute myeloid leukemia (AML) is a clonal disorder characterized by abnormal proliferation of myeloid cell precursors. Research has mainly focused on the cellular events, but the bone marrow matrix has attracted minor interest. In this study bone marrow biopsies were obtained from 35 newly diagnosed AML patients. The bone marrows were analyzed regarding the occurrence and distribution of hyaluronan (HYA) and reticulin fibers (type III collagen). The bone marrow sections were analyzed histochemically and compared with bone marrows from 30 healthy controls. The HYA staining was significantly stronger in the AML patients compared with the controls. Only one patient demonstrated abnormal reticulin staining score, but in the group of patients with antecedent myelodysplastic syndrome (MDS), the reticulin staining score was significantly higher compared with the patients with de novo AML. There was a significant correlation between the HYA staining and reticulin staining scores in the AML patients as was seen in the control group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Turley E, Noble P, Bourguignon L. Signaling properties of hyaluronan receptors. J Biol Chem 2002;277(7):4589–4592.

    Article  PubMed  CAS  Google Scholar 

  2. Horton M, et al. Hyaluronan fragments synergize with interferon to induce the C-X-C chemokines Mig and interferon-inducible protein-10 in mouse macrophages. J Biol Chem 1998;273(52):35088–35094.

    Article  PubMed  CAS  Google Scholar 

  3. Turley E. Purification of a hyaluronate-binding protein fraction that modifies cell social behavior. Biochem Biophys Res Commun 1982;108:1016–1024.

    Article  PubMed  CAS  Google Scholar 

  4. Underhill C. CD44: The hyaluronan receptor. J Cell Sci 1992;103:293–298.

    PubMed  CAS  Google Scholar 

  5. Greiner J, et al. Characterization of several leukemia-associated inducing humoral immune responses in acute and chronic myeloid leukemia. Internat J Cancer 2003;106:224–231.

    Article  CAS  Google Scholar 

  6. Ponta H, Sherman L, Herrlich P. CD44: From adhesion molecules to signaling regulators. Nature 2003;4:33–45.

    CAS  Google Scholar 

  7. Charrad R-S, et al. Ligation of the CD44 adhesion molecule reverses blockage of differentiation in human acute myeloid leukemia. Nature 1999;5(6):669–676.

    Article  CAS  Google Scholar 

  8. Politz O, et al. Stabilin-1 and-2 constitute a novel family of fasciclin-like hyaluronan receptor homologues. Biochem J 2002;362:155–164.

    Article  PubMed  CAS  Google Scholar 

  9. Islam A. Proposal for a classification of acute myeloid leukaemia based on plastic-embedded bone marrow biopsy sections. Leukemia Res 1993;17:421–427.

    Article  CAS  Google Scholar 

  10. Jaffe E, Harris N, Stein H, Vardiman J. (eds). World Health Organization Classification of Tumours. Pathology and genetics of tumours of haematopoetic and lymphoid tissues. IARC Press, Lyon, France, 2001, pp. 76–80.

    Google Scholar 

  11. Sundström G, Löfvenberg E, Hassan I, Engström-Laurent A. Localisation and distribution of hyaluronan in normal bone marrow matrix: a novel method to evaluate impending fibrosis? Eur J Haematol 2002;68:194–202.

    Article  PubMed  Google Scholar 

  12. Bain BJ, Clark DM, Lampert IA. Bone marrow pathology. Blackwell Science: Oxford, 2001.

    Google Scholar 

  13. Putt FA. Manual of histological staining methods. John Wiley & Sons: New York, 1972, p. 107.

    Google Scholar 

  14. Tengblad A. Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding proteins from cartilage. Biochim Biophys Acta 1979;578:281–289.

    PubMed  CAS  Google Scholar 

  15. Hellström S, Tengblad A, Johansson C, Hedlund U, Axelsson E. An improved technique for hyaluronan histiochemistry using microwave irradiation. Histochem J 1990;22:677–682.

    Article  PubMed  Google Scholar 

  16. Bauermeister DE. Quantitation of bone marrow reticulin—a normal range. Am J Clin Pathol 1971;56:24–31.

    PubMed  CAS  Google Scholar 

  17. Avigdor A, et al. CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood 103(8):2981–2989.

  18. Midura RJ, Xiaowei S, Morcuende JA, Tammi M, Tammi R. Parathyroid hormone rapidly stimulates hyaluronan synthesis by periosteal osteoblasts in the tibial diaphysis of the growing rat. J Biol Chem 2003;51(278):51462–51468.

    Article  Google Scholar 

  19. Hellström M, Mattsson C, Hellström S, Engström-Laurent A. Hyaluronan is differently located in arteries and veins. An immunohistochemical study in the rat. Cells Tissues Organs 2003;173:227–233.

    Article  PubMed  Google Scholar 

  20. Nilsson S, et al. Hyaluronan is synthesized by primitive hemopoetic cells, participates in their lodgment at the endosteum, following transplantation, and is involved in the regulation of their proliferation and differentiation in vitro. Blood 2003;101:856–862.

    Article  PubMed  CAS  Google Scholar 

  21. Li L, et al. Reconstitution of CD40 and CD80 in dendritic cells generated from blasts of patients with acute myeloid leukemia. Cancer Immunity 2003;3:8–19.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Section of Haematology, Norrlands Unvierksity Hospital, SE-901 85, Umeå, Sweden

    Gunnel Sundström MD & Anders Wahlin

  2. Section of Haematology, University Hospital of North-Norway, 9038, Tromsö, Norway

    Inger Marie S. Dahl

  3. Department of Medical Biosciences, Pathology, Umeå University, SE-901 85, Umeå, Sweden

    Magnus Hultdin

  4. Department of Clinical Science, Umeå University, SE-901 85, Umeå, Sweden

    Berith Lundström

  5. Department of Public Health and Clinical Medicine, SE-901 85, Umeå, Sweden

    Anna Engström-Laurent

Authors
  1. Gunnel Sundström MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Inger Marie S. Dahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Magnus Hultdin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Berith Lundström
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anders Wahlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Anna Engström-Laurent
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gunnel Sundström MD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sundström, G., Dahl, I.M.S., Hultdin, M. et al. Bone marrow hyaluronan distribution in patients with acute myeloid leukemia. Med Oncol 22, 71–78 (2005). https://doi.org/10.1385/MO:22:1:071

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/MO:22:1:071

Key Words

Search

Navigation