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A statistical view of FMRFamide neuropeptide diversity

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Abstract

FMRFamide-like peptide (FLP) amino acid sequences have been collected and statistically analyzed. FLP amino acid composition as a function of position in the peptide is graphically presented for several major phyla. Results of total amino acid composition and frequencies of pairs of FLP amino acids have been computed and compared with corresponding values from the entire GenBank protein sequence database. The data for pairwise distributions of amino acids should help in future structure-function studies of FLPs. To aid in future peptide discovery, a computer program and search protocol was developed to identify FLPs from the GenBank protein database without the use of keywords.

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References

  • Baratte B., Gras-Masse H., Ricart G., Bulet P., and Dhainaut-Courtois N. (1991) Isolation and characterization of authentic Phe-Met-Arg-Phen-NH2 and the novel Phe-Thr-Arg-Phe-NH2 peptide from Nereis diversicolor. Eur. J. Biochem. 198, 627–633.

    Article  PubMed  CAS  Google Scholar 

  • Carlacci L. and Edison A. S. (2000) Computational analysis of two similar neuropeptides yields distinct conformational ensembles. Proteins Stuct. Funct. Genet., 40, 367–377.

    Article  CAS  Google Scholar 

  • Chin A. C., Reynolds E. R., and Scheller R. H. (1990) Organization and expression of the Drosophila FMRFamide-related prohormone gene. DNA Cell Biol. 9, 263–271.

    PubMed  CAS  Google Scholar 

  • Chin G. J., Payza K., Price D. A., Greenberg M. J., and Doble K. E. (1994) Characterization and solubilization of the FMRFamide receptor of squid. Biol. Bull. 187, 185–199.

    Article  PubMed  CAS  Google Scholar 

  • Cowden C. and Stretton A. O. (1993) AF2, an Ascaris neuropeptide: isolation, sequence, and bioactivity. Peptides 14, 423–430.

    Article  PubMed  CAS  Google Scholar 

  • Cowden C. and Stretton A. O. (1995) Eight novel FMRFamide-like neuropeptides isolated from the nematode Ascaris suum. Peptides 16, 491–500.

    Article  PubMed  CAS  Google Scholar 

  • Cropper E. C., Brezina V., Vilim F. S., Harish O., Price D. A., Rosen S., et al. (1994) FRF peptides in the ARC neuromuscular system of Aplysia: purification and physiological actions. J. Neurophysiol. 72, 2181–2195.

    PubMed  CAS  Google Scholar 

  • Curry W. J., Shaw C., Johnston C. F., Thim L., and Buchanan K. D. (1992) Neuropeptide F: primary structure from the tubellarian, Artioposthia triangulata. Comp. Biochem. Physiol. 101C, 269–274.

    CAS  Google Scholar 

  • Darmer D., Schmutzler C., Diekhoff D., and Grimmelikhuijzen C. J. (1991) Primary structure of the precursor for the sea anemone neuropeptide Antho-RFamide (less than Glu-Gly-Arg-Phe-NH2). Proc. Natl. Acad. Sci. USA 88, 2555–2559.

    Article  PubMed  CAS  Google Scholar 

  • Darmer D., Hauser F., Nothacker H. P., Bosch T. C., Williamson M., and Grimmelikhuijzen C. J. (1998) Three different prohormones yield a variety of Hydra-RFamide (Arg-Phe-NH2) neuropeptides in Hydra magnipapillata. Biochem. J. 332, 403–412.

    PubMed  CAS  Google Scholar 

  • Davis, R. E. and Stretton, A. O. W. (1996) The motornervous system of Ascaris: electrophysiology and anatomy of the neurons and their control by neuromodulators. Parasitology 113, S97-S117.

    PubMed  Google Scholar 

  • Day T. A. and Maule A. G. (1999) Parasitic peptides! The structure and function of neuropeptides in parasitic worms. Peptides 20, 999–1019.

    Article  PubMed  CAS  Google Scholar 

  • de With N. D. and van der Schors R. C. (1992) SKPYMRFamide, a novel FMRFamide-related peptide in the snail Lymnaea stagnalis. Neuroreport 3, 612–614.

    Article  PubMed  Google Scholar 

  • Dockray G. J., Reevee J. R. Jr., Shively J., Gayton R. J., and Barnard C. S. (1983) A novel active pentapeptide from chicken brain identified by antibodies to FMRFamide. Nature 305, 328–330.

    Article  PubMed  CAS  Google Scholar 

  • Donly B. C., Fuse M., Orchard I., Tobe S. S., and Bendena W. G. (1996) Characterization of the gene for leucomyosuppressin and its expression in the brain of the cockroach Diploptera punctata. Insect. Biochem. Mol. Biol. 26, 627–637.

    Article  PubMed  CAS  Google Scholar 

  • Duve H., Johnsen A. H., Sewell J. C., Scott A. G., Orchard I., Rehfeld J. F., and Thorpe A. (1992) Isolation, structure, and activity of -Phe-Met-Arg-Phe-NH2 neuropeptides (designated calliFMRFamides from the blowfly Calliphora vomitoria. Proc. Natl. Acad. Sci. USA 89, 2326–2330.

    Article  PubMed  CAS  Google Scholar 

  • Edison A. S., Messinger L. A., and Stretton A. O. (1997) afp-1: a gene encoding multiple transcripts of a new class of FMRFamide-like neuropeptides in the nematode Ascaris suum. Peptides 18, 929–935.

    Article  PubMed  CAS  Google Scholar 

  • Edison, A. S., Espinoza, E., and Zachariah, C. (1999) Conformational ensembles: the role of neuropeptide structures in receptor binding. J. Neurosci. 19, 6318–6326.

    PubMed  CAS  Google Scholar 

  • Eipper B. A., Stoffers D. A., and Mains R. E. (1992) The biosynthesis of neuropeptides: peptide α-amidation. Annu. Rev. Neurosci. 15, 57–85.

    Article  PubMed  CAS  Google Scholar 

  • Evans B. D., Pohl J., Kartsonis N. A., and Calabrese R. L. (1991) Identification of RFamide neuropeptides in the medicinal leech. Peptides 12, 897–908.

    Article  PubMed  CAS  Google Scholar 

  • Favrel P., Lelong C., and Mathieu M. (1998) Structure of the cDNA encoding the precursor for the neuropeptide FMRFamide in the bivalve mollusc Mytilus edulis. Neuroreport 9, 2961–2965.

    Article  PubMed  CAS  Google Scholar 

  • Fonagy A., Schoofs L., Proost P., Van Damme J., Bueds H., and De Loof A. (1992a) 6Isolation, primary structure and synthesis of neomyosuppressin, a myoinhibiting neuropeptide from the grey fleshfly, Neobellieria bullata. Comp. Biochem. Physiol. 102C, 239–245.

    CAS  Google Scholar 

  • Fonagy A., Schoofs L., Proost P., Van Damme J., and De Loof A. (1992b) Isolation and primary structure of two sulfakinin-like peptides from the fleshfly, Neobellieria bullata. Comp. Biochem. Physiol. 103C, 135–142.

    CAS  Google Scholar 

  • Fujimoto K., Ohta N., Yoshida M., Kubota I., Muneoka Y., and Kobayashi M. (1990) A novel cardio-excitatory peptide isolated from the atria of the African giant snail, Achatina fulica. Biochem. Biophys. Res. Commun. 167, 777–783.

    Article  PubMed  CAS  Google Scholar 

  • Fujimoto M., Takeshita K. I., Wang X., Takabatake I., Fujisawa Y., Teranishi, H., et al. (1998) Isolation and characterization of a novel bioactive peptide, carassius RFamide (C-RFa), from the brain of the Japanese crucian carp. Biochem. Biophys. Res. Comm. 242, 436–440.

    Article  PubMed  CAS  Google Scholar 

  • Fujisawa Y., Ikeda T., Nomoto K., Yasuda-Kamatani Y., Minakata H., Kenny P. T., et al. (1992) The FMRFamide-related decapeptide of Mytilus contains a D-amino acid residue. Comp. Biochem. Physiol. 102C, 91–95.

    CAS  Google Scholar 

  • Gaus, G., Doble K. E., Price D. A., Greenberg M. J., Lee T. D., and Battelle B. A. (1993) The sequences of five neuropeptides isolated from Limulus using antisera to FMRFamide. Biol. Bull. 184, 322–329.

    Article  CAS  Google Scholar 

  • Gajewski M., Schmutzler C., and Plickert G. (1998) Structure of neuropeptide precursors in Cnidaria. Ann. NY Acad. Sci. 839, 311–315.

    Article  PubMed  CAS  Google Scholar 

  • Geary T. G., Price D. A., Bowman J. W., Winterrowd C. A., Mackenzie C. D., Garrison R. D., et al. (1992) Two FMRFamide-like peptides from the free-living nematode Panagrellus redivivus. Peptides 13, 209–214.

    Article  PubMed  CAS  Google Scholar 

  • Greenberg M. J. and Price, D. (1992) Relationships among the FMRFamide-like peptides. Prog. Brain Res. 92, 25–37.

    Article  PubMed  CAS  Google Scholar 

  • Grimmelikhuijzen C. J. and Graff D. (1986) Isolation of pyroGlu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide from sea anemones. Proc. Natl. Acad. Sci. USA 83, 9817–9821.

    Article  PubMed  CAS  Google Scholar 

  • Grimmelikhuijzen C. J., Hahn M., Rinehart K. L., and Spencer A. N. (1988) Isolation of pyroGlu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide), a novel neuropeptide from hydromedusae. Brain Res. 475, 198–203.

    Article  PubMed  CAS  Google Scholar 

  • Holman G. M., Cook B. J., and Nachman R. J. (1996) Isolation, primary structure and synthesis of leucomyosuppressin, an insect neuropeptide that inhibits spontaneous contractions of the cockroach hindgut. Comp. Biochem. Physiol. 85C, 329–333.

    Google Scholar 

  • Huang Y., Brown M. R., Lee T. D., and Crim J. W. (1998) RF-amide peptides isolated from the midgut of the corn earworm, Helicoverpa zea, resemble pancreatic polypeptide. Insect Biochem. Mol. Biol. 28, 345–356.

    Article  PubMed  CAS  Google Scholar 

  • Johnston R. N., Shaw C., Halton D. W., Verhaert P., and Baguna J. (1995) GYIRFamide: a novel FMRFamide-related peptide (FaRP) from the triclad turbellarian, Dugesia tigrina. Biochem. Biophys. Res. Commun. 209, 689–697.

    Article  PubMed  CAS  Google Scholar 

  • Johnston R. N., Shaw C., Halton D. W., Verhaert P., Blair K. L., Brennan G. P., et al. (1996) Isolation, localization, and bioactivity of the FMRFamide-related neuropeptides GYIRFamide and YIRFamide from the marine turbellarian Bdelloura candida. J. Neurochem. 67, 814–821.

    Article  PubMed  CAS  Google Scholar 

  • Kanda, T., Kuroki Y., Kubota I., Muneoka Y., and Kobayashi M. (1990) Neuropeptides isolated from the ganglia of a prosobranch mollusc, Fusinus ferrugineus, in Peptide Chemistry 1989, Yanaihara N., ed., Protein Research Foundation, Osaka, pp. 39–44.

    Google Scholar 

  • Keating C., Thorndyke M. C., Holden-Dye L., Williams R. G., and Walker R. J. (1995) The isolation of a FMRFamide-like peptide from the nematode Haemonchus contortus. Parasitology 111, 515–521.

    PubMed  CAS  Google Scholar 

  • Kellett E., Saunders S. E., Li K. W., Staddon J. W., Benjamin P. R., and Burke J. F. (1994) Genomic organization of the FMRFamide gene in Lymnaea: multiple exons encoding novel neuropeptides. J. Neurosci. 14, 6564–6570.

    PubMed  CAS  Google Scholar 

  • Kingan T. G., Teplow D. B., Phillips J. M., Riehm J. P., Rao K. R., Hildebrand J. G., et al. (1990) A new peptide in the FMRFamide family isolated from the CNS of the hawkmoth, Manduca sexta. Peptides 11, 849–856.

    Article  PubMed  CAS  Google Scholar 

  • Krajniak K. G. and Price D. A. (1990) Authentic FMRFamide is present in the polychaete Nereis virens. Peptides 11, 75–77.

    Article  PubMed  CAS  Google Scholar 

  • Krajniak K. G. (1991) The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides 12, 1295–1302.

    Article  PubMed  CAS  Google Scholar 

  • Kuroki Y., Kanda T., Kubota I., Ikeda T., Fujisawa Y., Minakata H., and Muneoka Y. (1993) FMRFamide-related peptides isolated from the prosobranch mollusc Fusinus ferrugineus. Acta Biol. Hung. 44, 41–44.

    PubMed  CAS  Google Scholar 

  • Lange A. B., Peeff N. M., and Orchard I. (1994) Isolation, sequence, and bioactivity of FMRFamide-related peptides from the locust ventral nerve cord. Peptides 15, 1089–1094.

    Article  PubMed  CAS  Google Scholar 

  • Leung P. S., Shaw C., Maule A. G., Thim L., Johnston C. F., and Irvine G. B. (1992) The primary structure of neuropeptide F (NPF) from the garden snail, Helix aspersa. Regul. Pept. 41, 71–81.

    Article  PubMed  CAS  Google Scholar 

  • Li C., Kim K., and Nelson L. S. (1999) FMRFamide-related neuropeptide gene family in Caenorhabditis elegans. Brain Res. 848, 26–34.

    Article  PubMed  CAS  Google Scholar 

  • Linacre A., Kellett E., Saunders S., Bright K., Benjamin P. R., and Burke J. F. (1990) Cardioactive neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and novel related peptides are encoded in multiple copies by a single gene in the snail Lymnaea stagnalis. J. Neurosci 10, 412–419.

    PubMed  CAS  Google Scholar 

  • Loi P. K. and Tublitz N. (1997) Molecular analysis of FMRFamide- and FMRFamide-related peptides (FaRPS) in the cuttlefish Sepia officinalis. J. Exp. Biol. 200, 1483–1489.

    PubMed  CAS  Google Scholar 

  • Lutz E. M., Macdonald M., Hettle S., Price D. A., Cottrell G. A., and Sommerville J. (1992) Structure of cDNA clones and genomic DNA encoding FMRFamide-related peptides (FaRPs) in Helix. Mol. Cell. Neurosci. 3, 373–382.

    Article  CAS  PubMed  Google Scholar 

  • MacLennan, A. J. and Shaw, G. (1993) A yeast SH2 domain. Trends Biochem. 18, 464–465.

    Article  CAS  Google Scholar 

  • Madrid K. P., Price D. A., Greenberg M. J., Khan H. R., and Saleuddin A. S. (1994) FMRFamide-related peptides from the kidney of the snail, Helisoma trivolvis. Peptides 15, 31–36.

    Article  PubMed  CAS  Google Scholar 

  • Marks N. J., Shaw C., Maule A. G., Davis J. P., Halton D. W., Verhaert P., et al. (1995) Isolation of AF2 (KHEYLRFamide) from Caenorhabditis elegans: evidence for the presence of more than one FMRFamide-related peptide-encoding gene. Biochem. Biophys. Res. Commun. 217, 845–851.

    Article  PubMed  CAS  Google Scholar 

  • Marks N. J., Maule A. G., Li C., Nelson L. S., Thompson D. P., Alexander-Bowman S., et al. (1999) Isolation, pharmacology and gene organization of KPSFVRFamide: a neuropeptide from Caenorhabditis elegans. Biochem. Biophys. Res Commun. 254, 222–230.

    Article  PubMed  CAS  Google Scholar 

  • Martin R. and Voigt K. H. (1987) The neurosecretory system of octopus vena cava. Experientia 43, 537–543.

    Article  CAS  Google Scholar 

  • Matsumoto S., Brown M. R., Crim J. W., Vigna S. R., and Lea A. O. (1989) Isolation and primary structure of neuropeptides from the mosquito, Aedes aegypti, immunoreactive to FMRFamide antiserum. Insect Biochem. 19, 277–283.

    Article  CAS  Google Scholar 

  • Maule A. G., Shaw C., Halton D. W., Brennan G. P., Johnston C. F., and Moore S. (1992) Neuropeptide F (Moniezia expansa): localization and characterization using specific antisera. Parasitology 105, 505–512.

    PubMed  CAS  Google Scholar 

  • Maule A., Shaw C., Halton D., and Thim L. (1993) GNFFRFamide: a novel FMRFamide-immunoreactive peptide isolated from the sheep tapeworm, Moniezia expansa. Biochem. Biophys. Res. Commun. 193, 1054–1060.

    Article  PubMed  CAS  Google Scholar 

  • Maule A. G., Shaw C., Bowman J. W., Halton D. W., Thompson D. P., Geary T. G., and Thim L. (1994a) The FMRFamide-like neuropeptide AF2 (Ascaris suum) is present in the free-living nematode, Panagrellus redivivus (Nematoda, Rhabditida). Parasitology 109, 351–356.

    Article  PubMed  CAS  Google Scholar 

  • Maule A. G., Shaw C., Bowman J. W., Halton D. W., Thompson D. P., Geary T. G., and Thim L. (1994b) KSAYMRFamide: a novel FMRFamide-related hepatapeptide from the free-living nematode, Panagrellus redivivus, which is myoactive in the parasitic nematode, Ascaris suum. Biochem. Biophys. Res. Commun. 200, 973–980.

    Article  PubMed  CAS  Google Scholar 

  • Maule A. G., Shaw C., Halton D. W., Curry W. J., and Thim L. (1994c) RYIRFamide: a turbellarian FMRFamide-related peptide (FaRP). Regul. Pept. 50, 37–43.

    Article  PubMed  CAS  Google Scholar 

  • Maule A. G., Shaw C., Bowman J. W., Halton D. W., Thompson D. P., Thim L., Kubiak T. M., Martin R. A., and Geary T. G. (1995) Isolation and preliminary biological characterization of KPNFIRFamide, a novel FMRFamide-related peptide from the free-living nematode, Panagrellus redivivus. Peptides 16, 87–93.

    Article  PubMed  CAS  Google Scholar 

  • Maule A. G., Bowman J. W., Thompson D. P., Marks N. J., Friedman A. R., and Geary T. G. (1996) FMRFamide-related peptides (FaRPs) in nematodes: occurrence and neuromuscular physiology. Parasitology 113, S119-S135.

    PubMed  Google Scholar 

  • Mercier A. J., Orchard I., TeBrugge V., and Skerrett M. (1993) Isolation of two FMRFamide-related peptides from crayfish pericardial organs. Peptides 14, 137–143.

    Article  PubMed  CAS  Google Scholar 

  • Moosler A., Rinehart K. L., and Grimmelikhuijzen C. J. (1996) Isolation of four novel neuropeptides, the hydra-RFamides I–IV, from Hydra magnipapillata. Biochem. Biophys. Res. Commun. 229, 596–602.

    Article  PubMed  CAS  Google Scholar 

  • Moosler A., Rinehart K. L., and Grimmelikhuijzen C. J. (1997) Isolation of three novel neuropeptides, the Cyanea-RFamides I–III, from Scyphomedusae. Biochem. Biophys. Res. Commun. 236, 743–749.

    Article  PubMed  CAS  Google Scholar 

  • Nachman R. J., Holman G. M., Haddon W. F., and Ling N. (1986) Leucosulfakinin, a sulfated insect neuropeptide with homology to gastrin and cholecystokinin. Science 234, 71–73.

    Article  PubMed  CAS  Google Scholar 

  • Nambu J. R., Murphy-Erdosh C., Andrews P. C., Feistner G. J., and Scheller R. H. (1988) Isolation and characterization of a Drosophila neuropeptide gene. Neuron 1, 55–61.

    Article  PubMed  CAS  Google Scholar 

  • Nelson L. S., Kim K., Memmott J. E., and Li C. (1998) FMRFamide-related gene family in the nematode, Caenorhabditis elegans. Mol. Brain Res. 58, 103–111.

    Article  PubMed  CAS  Google Scholar 

  • Nichols R., Schneuwly S. A., and Dixon J. E. (1988) Identification and characterization of a Drosophila homologue to the vertebrate neuropeptide cholecystokinin. J. Biol. Chem. 263, 12167–12170.

    PubMed  CAS  Google Scholar 

  • Nichols R. (1992) Isolation and structural characterization of Drosophila TDVDHVFLRFamide and FMRFamide-containing neural peptides. J. Mol. Neurosci. 3, 213–218.

    PubMed  CAS  Google Scholar 

  • Perry S. J., Yi-Kung Huang E., Cronk D., Bagust J., Sharma R., Walker R. J., et al. (1997) A human gene encoding morphine modulating peptides related to NPFF and FMRFamide. FEBS Lett. 409, 426–430.

    Article  PubMed  CAS  Google Scholar 

  • Payza, K. (1987) FMRFamide receptors in Helix aspersa. Peptides 8, 1065–1074.

    Article  PubMed  CAS  Google Scholar 

  • Payza, K., Greenberg, M. J. and Price, D. A. (1989) Further characterization of Helix FMRFamide receptors: kinetics, tissue distribution, and interactions with the endogenous heptapeptides. Peptides 10, 657–661.

    Article  PubMed  CAS  Google Scholar 

  • Price, D. A. and Greenberg, M. J. (1977) Structure of a molluscan cardioexcitatory neuropeptide. Science 197, 670–671.

    Article  PubMed  CAS  Google Scholar 

  • Price D. A. (1986) Evolution of a molluscan cardioexcitatory neuropeptide. Am. Zoologist 26, 1007–1015.

    CAS  Google Scholar 

  • Price D. A., Doble K. E., Lesser W., Greenberg M. J., Swiderek K. M., Lee T. D., et al. (1996) The peptide pQFYRFamide is encoded on the FMRFamide precursor of the snail Helix aspersa but does not activate the FMRFamidegated sodium current. Biol. Bull. 191, 341–352.

    Article  PubMed  CAS  Google Scholar 

  • Rajpara S. M., Garcia P. D., Roberts R., Eliassen J. C., Owens D. F., Maltby D., et al. (1992) Identification and molecular cloning of a neuropeptide Y homolog that produces prolonged inhibition in Aplysia neurons. Neuron 9, 505–513.

    Article  PubMed  CAS  Google Scholar 

  • Reinscheid R. K. and Grimmelikhuijzen C. J. (1994) Primary structure of the precursor for the anthozoan neuropeptide antho-RFamide from Renilla kollikeri: evidence for unusual processing enzymes. J. Neurochem. 62, 1214–1222.

    Article  PubMed  CAS  Google Scholar 

  • Robb S., Packman L. C., and Evans P. D. (1989) Isolation, primary structure and bioactivity of schistoflrf-amide, a FMRF-amide-like neuropeptide from the locust, Schistocerca gregaria. Biochem. Biophys. Res. Commun. 160, 850–856.

    Article  PubMed  CAS  Google Scholar 

  • Rosoff M. L., Burglin T. R., and Li C. (1992) Alternatively spliced transcripts of the flp-1 gene encode distinct FMRFamide-like peptides in Caenorhabditis elegans. J. Neurosci. 12, 2356–2361.

    PubMed  CAS  Google Scholar 

  • Salzet M., Bulet P., Wattez C., and Malecha J. (1994) FMRFamide-related peptides in the sex segmental ganglia of the Pharyngobdellid leech Erpobdella octoculata. Identification and involvement in the control of hydric balance. Eur. J. Biochem. 221, 269–275.

    Article  PubMed  CAS  Google Scholar 

  • Schaefer M., Picciotto M. R., Kreiner T., Kaldany R. R., Taussig R., and Scheller R. H. (1985) Aplysia neurons express a gene encoding multiple FMRFamide neuropeptides. Cell 41, 457–67.

    Article  PubMed  CAS  Google Scholar 

  • Schinkmann K. and Li C. (1994) Comparison of two Caenorhabditis genes encoding FMRFamide(Phe-Met-Arg-Phe-NH2)-like peptides. Mol. Brain Res. 24, 238–246.

    Article  PubMed  CAS  Google Scholar 

  • Schmutzler C., Darmer D., Diekhoff D., and Grimmelikhuijzen C. J. (1992) Identification of a novel type of processing sites in the precursor for the sea anemone neuropeptide AnthoRFamide (<Glu-Gly-Arg-Phe-NH2) from Anthopleura elegantissima. J. Biol. Chem. 267, 22,534–22,541.

    CAS  Google Scholar 

  • Schmutzler C., Diekhoff D., and Grimmelikhuijzen C. J. (1994) The primary structure of the PolRFamide neuropeptide precursor protein from the hydromedusa Polyorchis penicillatus indicates a novel processing proteinase activity. Biochem. J. 299, 431–436.

    PubMed  CAS  Google Scholar 

  • Schneider L. E. and Taghert P. H. (1988) Isolation and characterization of a Drosophila gene that encodes multiple neuropeptides related to Phe-Met-Arg-Phe-NH2 (FMRFamide). Proc. Natl. Acad. Sci. USA 85, 1993–1997.

    Article  PubMed  CAS  Google Scholar 

  • Schoofs L., Holman, G. M., Hayes, T. K., Nachman, R. J., and De Loof, A. (1990) Isolation and identification of a sulfakinin-like peptide with sequence homology to vertebrate gastrin and cholecystokinin, from the brain of Locusta migratoria, in Chromatography and Isolation of Insect Hormones and Pheromones, McCaffery, A. and Wilson, I., eds., Plenum, New York, pp. 231–241.

    Google Scholar 

  • Schoofs L, Holman G. M., Paemen L., Veelaert D., Amelinckx M., and De Loof A. (1993) Isolation, identification, and synthesis of PDVDHFLRFamide (SchistoFLRFamide) in Locusta migratoria and its association with the male accessory glands, the salivary glands, the heart, and the oviduct. Peptides 14, 409–421.

    Article  PubMed  CAS  Google Scholar 

  • Schoofs L., Veelaert D., Vanden Broeck J., and De Loof A. (1997) Peptides in the locusts, Locusta migratoria and Schistocerca gregaria. Peptides. 18, 145–156.

    Article  PubMed  CAS  Google Scholar 

  • Shaw, G. (1993a) Rapid identification of proteins. Proc. Natl. Acad. Sci. USA 90, 5138–5142.

    Article  PubMed  CAS  Google Scholar 

  • Shaw, G. (1993b) Identification of novel pleckstrin homology (PH) domains provide a hypothesisfor PH domain function. Biochem. Biophys. Res. Comm. 195, 1145–1151.

    Article  PubMed  CAS  Google Scholar 

  • Smart D., Shaw C., Johnston C., Thim L., Halton D. and Buchanan K. (1992) Peptide tyrosine phenylalanine. A novel neuropeptide F-related nonapeptide from the brain of the squid, Loligo vulgaris. Biochem. Biophys. Res. Commun. 186, 1616–1623.

    Article  PubMed  CAS  Google Scholar 

  • Spittaels K., Verhaert P., Shaw C., Johnston R. N., Devreese B., Van Beeumen J., and De Loof A. (1996) Insect neuropeptide F (NPF)-related peptides: isolation from Colorado potato beetle (Leptinotarsa decemlineata) brain. Insect Biochem. Mol. Biol. 26, 375–382.

    Article  PubMed  CAS  Google Scholar 

  • Stretton, A. O. W., Donmoyer, J., Davis, R., Meade, J., Cowden, C., and Sithigorngul, P. (1992) Motor behavior and motor nervous system function in the nematode Ascaris suum. J. Parasitol. 78, 206–214.

    Article  PubMed  CAS  Google Scholar 

  • Taghert P. H. and Schneider L. E. (1990) Interspecific comparison of a Drosophila gene encoding FMRFamide-related neuropeptides. J. Neurosci. 10, 1929–1942.

    PubMed  CAS  Google Scholar 

  • Taghert P. H. (1999) FMRFamide neuropeptides and neuropeptide-associated enzymes in Drosophila. Microsc Res Tech. 45, 80–95.

    Article  PubMed  CAS  Google Scholar 

  • Taussing R. and Scheller R. H. (1986) The Aplysia FMRFamide gene encodes sequences related to mammalian brain peptides. DNA 5, 453–461.

    Google Scholar 

  • Trimmer B. A., Kobierski L. A., and Kravitz E. A. (1987) Purification and characterization of FMRFamidelike immunoreactive substances from the lobster nervous system: isolation and sequence analysis of two closely related peptides. J. Comp. Neurol. 266, 16–26.

    Article  PubMed  CAS  Google Scholar 

  • Veenstra J. A. (1989) Isolation and structure of two gastrin/CCK-like neuropeptides from the American cockroach homologous to the leucosulfakinins. Neuropeptides 14, 145–149.

    Article  PubMed  CAS  Google Scholar 

  • Veenstra J. A. and Lambrou G. (1995) Isolation of a novel RFamide peptide from the midgut of the American cockroach, Periplaneta americana. Biochem. Biophys. Res. Commun. 213, 519–524.

    Article  PubMed  CAS  Google Scholar 

  • Veenstra J. A. (1999) Isolation and identification of three RFamide-immunoreactive peptides from the mosquito Aedes aegypti. Peptides 20, 31–38.

    Article  PubMed  CAS  Google Scholar 

  • Vilim F. S., Aarnisalo A. A., Nieminen M. L., Lintunen M., Karlstedt K., Kontinen V. K., Kalso E., States B., Panula P., and Ziff E. (1999) Gene for pain modulatory neuropeptide NPFF: induction in spinal cord by noxious stimuli. Mol. Pharmacol. 55, 804–811.

    PubMed  CAS  Google Scholar 

  • Voigt K. H., Kiehling C., Geis R., Falke N., and Martin R. (1983) Identification of Metenkephalin-Arg6-Phe7-amide: an opiod and cardioexcitatory neuropeptide from the mollusc. Proc. Int. Narcotic Res. Conf., F. R. G., June 26–July 1. 52L.

  • Weimann J. M., Marder E., Evans B., and Calabrese R. L. (1993) The effects of SDRNFLRFamide and TDRNFLRFamide on the motor patterns of the stomatogastric ganglion of the crab Cancer borealis. J. Exp. Biol. 181, 1–26.

    PubMed  CAS  Google Scholar 

  • Wilmot C. M. and Thornton, J. M. Analysis and prediction of the different types of b-turn in proteins. J. Mol. Biol. 203, 221–232 (1988).

    Article  PubMed  CAS  Google Scholar 

  • Yang H. Y., Fratta W., Majane E. A., and Costa E. (1985) Isolation, sequencing, synthesis, and pharmacological characterization of two brain neuropeptides that modulate the action of morphine. Proc. Natl. Acad. Sci. USA 82, 7757–7761.

    Article  PubMed  CAS  Google Scholar 

  • Yasuda A., Naya Y., and Nakanishi K. (1993) Isolation of Antho-RFamide related peptides from the eyestalks of blue crab. Comp. Biochem. Physiol. 104B, 235–240.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Florida, 32610, Gainesville, FL

    Eduardo Espinoza, Stephen G. Thomas & Arthur S. Edison

  2. Graduate Program in Neuroscience, University of Florida, 32610, Gainesville, FL

    Matthew Carrigan, Gerry Shaw & Arthur S. Edison

  3. The University of Florida Brain Institute, University of Florida, 32610, Gainesville, FL

    Gerry Shaw & Arthur S. Edison

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  1. Eduardo Espinoza
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  2. Matthew Carrigan
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  3. Stephen G. Thomas
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  4. Gerry Shaw
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  5. Arthur S. Edison
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Correspondence to Arthur S. Edison.

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Espinoza, E., Carrigan, M., Thomas, S.G. et al. A statistical view of FMRFamide neuropeptide diversity. Mol Neurobiol 21, 35–56 (2000). https://doi.org/10.1385/MN:21:1-2:035

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