Abstract
The development of immune-mediated diabetes in BB rats may involve a defect of the gastrointestinal tract (GI), as suggested by increased gut permeability. This study aimed at measuring invertase, maltase, lactase, and peroxidase activities in the duodenum of diabetes-prone BioBreeding (BBdp) rats and control BioBreeding rats (BBc) given free access to NIH-07 diet up to the time of killing at 60–66 d of age. After washing the entire small intestine, the duodenal mucosa was scraped off in the first 5-cm segment from the pylorus and frozen in distilled water. Invertase, maltase, and lactase activities were measured by monitoring the conversion of [U-14C]sucrose, [U-14C]maltose, and [d-[1-14C]glucose] lactose to radioactive hexoses, which were phosphorylated in the presence of adenosine triphosphatase and yeast hexokinase and then separated from their precursor by ion-exchange chromatography. Peroxidase activity was measured by a spectrophotometric procedure. In the BBdp rats, the activity of invertase, maltase, and lactase averaged, respectively, 70.2±4.4, 81.2±4.3, and 75.7±4.1% (n=16 and p<0.001 in all cases) of the control values found in BBc rats of the same sex. Inversely, after exclusion of two female BBc rats with abnormally high plasma d-glucose concentration, the activity of peroxidase in the BBdp rats averaged 157.4±20.0% (n=16; p<0.02) of the mean control value recorded in BBc rats of the same sex (100.0±9.3%; n=14). These findings are compatible with the view that a proinflammatory state of the GI associated with compromise function may precede the occurrence of pancreatic insulitis in BBdp rats and, possibly, human subjects with type 1 diabetes.
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Courtois, P., Meuris, S., Sener, A. et al. Invertase, maltase, lactase, and peroxidase activities in duodenum of BB rats. Endocr 19, 293–299 (2002). https://doi.org/10.1385/ENDO:19:3:293
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DOI: https://doi.org/10.1385/ENDO:19:3:293