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Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy

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Abstract

The objective of the study was to demonstrate the mRNA expression of estrogen receptor α (ERα), ERβ, and progesterone receptor (PR) by block reverse transcription-polymerase chain reaction(RT-PCR) and real-time RT-PCR (LightCycler) in bovine ovarian follicles and in corpus luteum during the estrous cycle and pregnancy. The mRNA expression of ERα and ERβ mRNA in theca interna tissue (TI) (lower pg/µg RNA) increased continuously and significantly during final growth of follicles, with much higher levels for ERα. The mRNA expression of ERα and ERβ in granulosa cells (GC) (fg/µg RNA) increased continuously during follicle growth but without any significant change. The expression of mRNA for PR in follicles (lower fg/µg RNA) increased continuously to maximum level in preovulatory follicles with a significant change only in TI. The highest mRNA expression for ERα (fg/µg RNA) was detected in corpus luteum (CL) during the early luteal phase, following by a significant decrease of expression during the mid, late, and regression phases. In contrast, ERβ mRNA expression is relatively high during the early stage, decreased during the late early and mid luteal phase, and increased significantly again during the late luteal phase and after CL regression. During pregnancy (>3 mo), low levels of ERα and ERβ mRNA expression (<25 fg/µg RNA) with no significant changes were measured. No significant change in PR mRNA expression (levels <13 fg/µg RNA) during the estrous cycle and pregnancy in bovine CL were found. The results suggest an autocrine/paracrine role of steroid receptors in the regulation of final follicle growth and corpus luteum formation and function.

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References

  1. Conneely, O. M. (2001). Endocrinology 142, 2194–2199.

    Article  PubMed  CAS  Google Scholar 

  2. Mosselman, S., Polman, J., and Dijkema, R. (1996). FEBS Lett. 392, 49–53.

    Article  PubMed  CAS  Google Scholar 

  3. Kuiper, G. G., Enmark, E., Pelto-Huikko, M., Nilsson, S., and Gustafsson, J. A. (1996). Proc. Natl. Acad. Sci. USA 93, 5925–5930.

    Article  PubMed  CAS  Google Scholar 

  4. Tremblay, G. B., Tremblay, A., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Labrie, F., et al. (1997). Mol. Endocrinol. 11, 353–365.

    Article  PubMed  CAS  Google Scholar 

  5. Walter, P., Green, S., Greene, G., Krust, A., Bornert, J. M., Jeltsch, J. M., et al. (1985). Proc. Natl. Acad. Sci. USA 82, 7889–7893.

    Article  PubMed  CAS  Google Scholar 

  6. Green, S., Walter, P., Greene, G., Krust, A., Goffin, C., Jensen, E., et al. (1986). J. Steroid Biochem. 24, 77–83.

    Article  PubMed  CAS  Google Scholar 

  7. Kuiper, G. G., Lemmen, J. G., Carlsson, B., Corton, J. C., Safe, S. H., van der Saag, P. T., et al. (1998). Endocrinology 139, 4252–4263.

    Article  PubMed  CAS  Google Scholar 

  8. Gustafsson, J. A. (1999). J. Endocrinol. 163, 379–383.

    Article  PubMed  CAS  Google Scholar 

  9. Rosenfeld, C. S., Yuan, X., Manikkam, M., Calder, M. D., Garverick, H. A., and Lubahn, D. B. (1999). Biol. Reprod. 60, 691–697.

    Article  PubMed  CAS  Google Scholar 

  10. Shughrue, P. J., Komm, B., and Merchenthaler, I. (1996). Steroids 61, 678–681.

    Article  PubMed  CAS  Google Scholar 

  11. Shughrue, P. J., Lane, M. V., Scrimo, P. J., and Merchenthaler, I. (1998). Steroids 63, 498–504.

    Article  PubMed  CAS  Google Scholar 

  12. Mowa, C. N. and Iwanaga, T. (2000). J. Endocrinol. 165, 59–66.

    Article  PubMed  CAS  Google Scholar 

  13. Conneely, O. M., Maxwell, B. L., Toft, D. O., Schrader, W. T., and O’Malley, B. W. (1987). Biochem. Biophys. Res. Commun. 149, 493–501.

    Article  PubMed  CAS  Google Scholar 

  14. Shyamala, G., Schneider, W., and Schott, D. (1990). Endocrinology 126, 2882–2889.

    PubMed  CAS  Google Scholar 

  15. Giangrande, P. H. and McDonnell, D. P. (1999). Recent Prog. Horm. Res. 54, 291–314.

    PubMed  CAS  Google Scholar 

  16. Duffy, D. M., Wells, T. R., Haluska, G. J., and Stouffer, R. L. (1997). Biol. Reprod. 57, 693–699.

    Article  PubMed  CAS  Google Scholar 

  17. Conneely, O. M., Mulac-Jericevic, B., Lydon, J. P., and De Mayo, F. J. (2001). Mol. Cell. Endocrinol. 179, 97–103.

    Article  PubMed  CAS  Google Scholar 

  18. Jansen, H. T., West, C., Lehman, M. N., and Padmanabhan, V. (2001). Biol. Reprod. 65, 866–872.

    Article  PubMed  CAS  Google Scholar 

  19. Tomanek, M., Pisselet, C., Monget, P., Madigou, T., Thieulant, M. L., and Monniaux, D. (1997). Mol. Reprod. Dev. 48, 53–62.

    Article  PubMed  CAS  Google Scholar 

  20. Slomczynska, M. and Wozniak, J. (2001). Exp. Clin. Endocrinol. Diabetes 109, 238–244.

    Article  PubMed  CAS  Google Scholar 

  21. Walther, N., Lioutas, C., Tillmann, G., and Ivell, R. (1999). Mol. Cell. Endocrinol. 152, 37–45.

    Article  PubMed  CAS  Google Scholar 

  22. Cardenas, H., Burke, K. A., Bigsby, R. M., Pope, W. F., and Nephew, K. P. (2001). Biol. Reprod. 65, 128–134.

    Article  PubMed  CAS  Google Scholar 

  23. Iwai, T., Nanbu, Y., Iwai, M., Taii, S., Fujii, S., and Mori, T. (1990). Virchows Arch. A Pathol. Anat. Histopathol. 417, 369–375.

    Article  PubMed  CAS  Google Scholar 

  24. Misao, R., Nakanishi, Y., Fujimoto, J., and Tamaya, T. (1998). Horm. Res. 50, 155–159.

    Article  PubMed  CAS  Google Scholar 

  25. Park-Sarge, O. K., Parmer, T. G., Gu, Y. and Gibori, G. (1995). Endocrinology 136, 1537–1543.

    Article  PubMed  CAS  Google Scholar 

  26. Natraj, U. and Richards, J. S. (1993). Endocrinology 133, 761–769.

    Article  PubMed  CAS  Google Scholar 

  27. Cassar, C. A., Dow, M. P. D., Pursley, J. R., and Smith, G. W. (2001). Biol. Reprod. 64(Suppl. 1), 162–163.

    Google Scholar 

  28. Rueda, B. R., Hendry, I. R., Hendry, W. J., Stormshak, F., Slayden, O. D., and Davis, J. S. (2000). Biol. Reprod. 62, 269–276.

    Article  PubMed  CAS  Google Scholar 

  29. Jo, M., Komar, C. M., and Fortune, J. E. (2001). Biol. Reprod. 64(Suppl. 1), 163–163.

    Google Scholar 

  30. Smith, G. W., Gentry, P. C., Long, D. K., Bao, B., Roberts, R. M., and Smith, M. F. (1995). Biol. Reprod. 52(Suppl. 1), 151.

    Google Scholar 

  31. Pfaffl, M. W., Lange, I. G., Daxenberger, A., and Meyer, H. H. D. (2001). APMIS 109, 345–355.

    Article  PubMed  CAS  Google Scholar 

  32. Pfaffl, M., Daxenberger, A., Hageleit, M., and Meyer, H. H. D. (2002). J. Vet. Med. A. Physiol. Pathol. Clin. Med. 49, 57–64.

    PubMed  CAS  Google Scholar 

  33. Fortune, J. E. and Hansel, W. (1979). Endocrinology 104, 1834–1838.

    PubMed  CAS  Google Scholar 

  34. Leung, P. C. and Armstrong, D. T. (1980). Life Sci. 27, 415–420.

    Article  PubMed  CAS  Google Scholar 

  35. Spicer, L. J. and Hammond, J. M. (1987). Mol. Cell. Endocrinol. 50, 139–147.

    Article  PubMed  CAS  Google Scholar 

  36. Lino, J., Baranao, S., and Hammond, J. M. (1985). Endocrinology 116, 2143–2151.

    PubMed  CAS  Google Scholar 

  37. Fortune, J. E. (1986). Biol. Reprod. 35, 292–299.

    Article  PubMed  CAS  Google Scholar 

  38. Roberts, A. J. and Skinner, M. K. (1990). Endocrinology 127, 2918–2929.

    PubMed  CAS  Google Scholar 

  39. Wu, W. X., Owiny, J., Zhang, Q., Ma, X. H., and Nathanielsz, P. W. (1996). Biol. Reprod. 55, 762–768.

    Article  PubMed  CAS  Google Scholar 

  40. Mueller, M. D., Vigne, J.-L., Minchenko, A., Lebovic, D. I., Leitman, D. C., and Taylor, R. N. (2000). Proc. Natl. Acad. Sci. USA 97, 10,972–10,977.

    CAS  Google Scholar 

  41. Berisha, B., Schams, D., Kosmann, M., Amselgruber, W., and Einspanier, R. (2000). J. Endocrinol. 167, 371–382.

    Article  PubMed  CAS  Google Scholar 

  42. Richards, J. S., Ireland, J. J., Rao, M. C., Bernath, G. A., Midgley, A. R. Jr., and Reichert, L. E. Jr. (1976). Endocrinology 99, 1562–1570.

    Article  PubMed  CAS  Google Scholar 

  43. Saji, S., Sakaguchi, H., Andersson, S., Warner, M., and Gustafsson, J. A. (2001). Endocrinology 142, 3177–3186.

    Article  PubMed  CAS  Google Scholar 

  44. Zieba, D., Bilinska, B., Schmalz-Fracek, B., and Murawski, M. (2000). Folia Histochem. Cytobiol. 38, 111–117.

    PubMed  CAS  Google Scholar 

  45. Kimball, F. A. and Hansel, W. (1974). Biol. Reprod. 11, 566–577.

    Article  PubMed  CAS  Google Scholar 

  46. Kobayashi, S., Miyamoto, A., Berisha, B., and Schams, D. (2001). Prostaglandins Other Lipid Mediat. 63, 79–92.

    Article  PubMed  CAS  Google Scholar 

  47. Okuda, K., Uenoyama, Y., Berisha, B., Lange, I. G., Taniguchi, H., Kobayashi, S., et al. (2001). Biol. Reprod. 65, 1634–1639.

    Article  PubMed  CAS  Google Scholar 

  48. Liebermann, J. and Schams, D. (1995). J. Reprod. Dev. 41, 187–198.

    Article  CAS  Google Scholar 

  49. Guo, I. C., Wu, L. S., Lin, J. H., and Chung, B. C. (2001). Life Sci. 68, 1851–1865.

    Article  PubMed  CAS  Google Scholar 

  50. Jarry, H., Einspanier, A., Kanngiesser, L., Dietrich, M., Pitzel, L., Holtz, W., et al. (1990). Endocrinology 126, 2350–2358.

    Article  PubMed  CAS  Google Scholar 

  51. Meyer, H. H. D., Mittermeier, T., and Schams, D. (1988). Acta Endocrinol. (Copenh.) 118, 96–104.

    CAS  Google Scholar 

  52. Skarzynski, D. J. and Okuda, K. (1999). Biol. Reprod. 60, 1292–1208.

    Article  PubMed  CAS  Google Scholar 

  53. Skarzynski, D. J., Jaroszeweski, J. J., and Okuda, K. (2001). J. Reprod. Dev. 47, 125–137.

    Article  CAS  Google Scholar 

  54. Rae, M. T., Menzies, G. S., McNeilly, A. S., Woad, K., Webb, R., and Bramley, T. A. (1998). Biol. Reprod. 58, 1394–1406.

    Article  PubMed  CAS  Google Scholar 

  55. Ireland, J., Murphee, R., and Coulson, P. (1991). J. Dairy Sci. 63, 166–160.

    Google Scholar 

  56. Berisha, B., Schams, D., Kosmann, M., Amselgruber, W., and Einspanier, R. (2000). Biol. Reprod. 63, 1106–1114.

    Article  PubMed  CAS  Google Scholar 

  57. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J., and Rutter, W. J. (1979). Biochemistry 18, 5294–5299.

    Article  PubMed  CAS  Google Scholar 

  58. Pfaffl, M. W. (2001). In: Rapid cycle real-time PCR, methods and applications. Meuer, S., Wittwer, D., and Nakagawara, K. (eds.). Springer-Verlag: Heidelberg, pp. 281–291.

    Google Scholar 

  59. Prakash, B. S., Meyer, H. H. D., Schallenberger, E., and van de Weil, D. F. (1987). J. Steroid Biochem. 28, 623–627.

    Article  PubMed  CAS  Google Scholar 

  60. Meyer, H. H. D., Sauerwein, H., and Mutyaoba, B. M. (1990). J. Steroid Biochem. 35, 263–269.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Physiology, Technical University Munich-Weihenstephan, D-85350, Freising, Germany

    Bajram Berisha, Michael W. Pfaffl &  Dieter Schams

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  1. Bajram Berisha
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  2. Michael W. Pfaffl
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  3. Dieter Schams
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Correspondence to Dieter Schams.

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Berisha, B., Pfaffl, M.W. & Schams, D. Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy. Endocr 17, 207–214 (2002). https://doi.org/10.1385/ENDO:17:3:207

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  • DOI: https://doi.org/10.1385/ENDO:17:3:207

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