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Preserved coupling of oxidative phosphorylation but decreased mitochondrial respiratory capacity in IL-1β-treated human peritoneal mesothelial cells

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Abstract

The peritoneal mesothelium acts as a regulator of serosal responses to injury, infection, and neoplastic diseases. After inflammation of the serosal surfaces, proinflammatory cytokines induce an “activated” mesothelial cell phenotype, the mitochondrial aspect of which has not previously been studied. After incubation of cultured human peritoneal mesothelial cells with interleukin (IL)-1β for 48 h, respiratory activity of suspended cells was analyzed by high-resolution respirometry. Citrate synthase (CS) and lactate dehydrogenase (LDH) activities were determined by spectrophotometry. Treatment with IL-1β resulted in a significant decline of respiratory capacity (p<0.05). Respiratory control ratios (i.e., uncoupled respiration at optimum carbonyl cyanide p-trifluoromethoxyphenylhydrazone concentration divided by oligomycin inhibited respiration measured in unpermeabilized cells) remained as high as 11, indicating well-coupled mitochondria and functional integrity of the inner mitochondrial membrane. Whereas respiratory capacities of the cells declined in proportion with decreased CS activity (p<0.05), LDH activity increased (p<0.05). Taken together, these results indicate that IL-1β exposure of peritoneal mesothelial cells does not lead to irreversible defects or inhibition of specific components of the respiratory chain, but is associated with a decrease of mitochondrial content of the cells that is correlated with an increase in LDH (and thus glycolytic) capacity.

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Authors and Affiliations

  1. Department of Pathological Anatomy, University Hospital Innsbruck, Austria

    Sylvia Stadlmann & Patrizia Lucia Moser

  2. Department of Pathophysiology, Innsbruck Medical University, Austria

    Kathrin Renner

  3. Institute of Ecology and Conservation Biology, University of Vienna, Austria

    Juergen Pollheimer

  4. Department of Obstetrics and Gynecology, University Hospital Innsbruck, Austria

    Alain Gustave Zeimet

  5. Department of Pathology, Academic Teaching Hospital Feldkirch, Austria

    Felix Albert Offner

  6. Department of General and Transplant Surgery, D. Swarovsky Research Laboratory, Innsbruck Medical University, Innrain 66/6, A, 6020, Innsbruck, Austria

    Erich Gnaiger

Authors
  1. Sylvia Stadlmann
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  2. Kathrin Renner
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  3. Juergen Pollheimer
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  4. Patrizia Lucia Moser
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  5. Alain Gustave Zeimet
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  6. Felix Albert Offner
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  7. Erich Gnaiger
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Correspondence to Erich Gnaiger.

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Contributed equally to this work.

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Stadlmann, S., Renner, K., Pollheimer, J. et al. Preserved coupling of oxidative phosphorylation but decreased mitochondrial respiratory capacity in IL-1β-treated human peritoneal mesothelial cells. Cell Biochem Biophys 44, 179–186 (2006). https://doi.org/10.1385/CBB:44:2:179

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