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Selection of anion exchangers for detoxification of dilute-acid hydrolysates from spruce

  • Session 3—Bioprocessing, Including Separations
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Abstract

Six anion-exchange resins with different properties were compared with respect to detoxification of a dilute-acid hydrolysate of spruce prior to ethanolic fermentation with Saccharomyces cerevisiae. The six resins encompassed strong and weak functional groups as well as styrene-, phenol-, and acrylic-based matrices. In an analytical experimental series, fractions from columns packed with the different resins were analyzed regarding pH, glucose, furfural, hydroxymethylfurfural, phenolic compounds, levulinic acid, acetic acid, formic acid, and sulfate. An initial adsorption of glucose occurred in the strong alkaline environment and led to glucose accumulation at a later stage. Acetic and levulinic acid passed through the column before formic acid, whereas sulfate had the strongest affinity. In a preparative experimental series, one fraction from each of six columns packed with the different resins was collected for assay of the fermentability and analysis of glucose, mannose, and fermentation inhibitors. The fractions collected from strong anion-exchange resins with styrene-based matrices displayed the best fermentability: a sevenfold enhancement of ethanol productivity compared with untreated hydrolysate. Fractions from a strong anion exchanger with acrylic-based matrix and a weak exchanger with phenol-based resin displayed an intermediate improvement in fermentability, a four- to fivefold increase in ethanol productivity. The fractions from two weak exchangers with styrene- and acrylic-based matrices displayed a twofold increase in ethanol productivity. Phenolic compounds were more efficiently removed by resins with styrene-and phenol-based matrices than by resins with acrylic-based matrices.

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Authors and Affiliations

  1. Biochemistyr, Division for Chemistry, Karlstad University, SE-651 88, Karlstad, Sweden

    Ilona Sárvári Horváth & Leif J. Jönsson

  2. Institute of Chemical Engineering and Environmental Sciences, Department of Chemical Reaction Engineering, Chalmers University, of Technology, SE-412 96, Göteborg, Sweden

    Ilona Sárvári Horváth

  3. STFI AB, Swedish Pulp and Paper Research Institute, PO Box 5604, SE-114 86, Stockholm, Sweden

    Anders Sjöde & Nils-Olof Nilvebrant

  4. Department of Material Science and Engineering, Royal Institute of Technology, KTH, SE-100 44, Stockholm, Sweden

    Andrei Zagorodni

Authors
  1. Ilona Sárvári Horváth
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  2. Anders Sjöde
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  3. Nils-Olof Nilvebrant
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  4. Andrei Zagorodni
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  5. Leif J. Jönsson
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Correspondence to Leif J. Jönsson.

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Sárvári Horváth, I., Sjöde, A., Nilvebrant, NO. et al. Selection of anion exchangers for detoxification of dilute-acid hydrolysates from spruce. Appl Biochem Biotechnol 114, 525–538 (2004). https://doi.org/10.1385/ABAB:114:1-3:525

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  • DOI: https://doi.org/10.1385/ABAB:114:1-3:525

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