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Production of medium-chain-length polyhydroxyalkanoates by pseudomonas aeruginosa with fatty acids and alternative carbon sources

  • Session 6 Bioprocess Research and Development
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Abstract

In this study, medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were produced by Pseudomonas aeruginosa using different carbon sources. Decanoic acid induced the highest (9.71% [±0.7]) mcl-PHAs accumulation in bacterial cells at 47 h. The cells preferred to accumulate and degrade the poly-hydroxyoctanoate than polyhydroxydecanoate (PHD) during early stage and final stage, of the growth, respectively. The production cost of mcl-PHAs can be reduced by using edible oils as the carbon source. The bacteria accumulated 6% (±0.7) of mcl-PHAs in the presence of olive oil. Besides, reused oil was another potential carbon source for the reduction of the production cost of mcl-PHAs. Overall, PHD was the major constitutent in the accumulated mcl-PHAs.

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Authors and Affiliations

  1. State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China

    Pui-Ling Chan, Vincent Yu, Lam Wai & Hoi-Fu Yu

  2. Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China

    Pui-Ling Chan, Vincent Yu, Lam Wai & Hoi-Fu Yu

Authors
  1. Pui-Ling Chan
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  2. Vincent Yu
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  3. Lam Wai
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  4. Hoi-Fu Yu
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Correspondence to Hoi-Fu Yu.

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Chan, PL., Yu, V., Wai, L. et al. Production of medium-chain-length polyhydroxyalkanoates by pseudomonas aeruginosa with fatty acids and alternative carbon sources. Appl Biochem Biotechnol 132, 933–941 (2006). https://doi.org/10.1385/ABAB:132:1:933

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  • DOI: https://doi.org/10.1385/ABAB:132:1:933

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