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In Vivo Analysis of Trophoblast Cell Invasion in the Human

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Placenta and Trophoblast

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 122))

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Abstract

In vivo analysis of trophoblast cell invasion is highly dependent on histological techniques, which are amply described in standard textbooks. The emphasis of this chapter therefore lies on material collection and interpretation of tissue sections, rather than on histological techniques per se. Proper identification of vascular structures on placental bed histological sections is important, the more because invading trophoblastic cells induce significant structural changes in uterine blood vessels, which may be disturbed in complicated pregnancies. Guidelines for distinguishing several vascular structures are provided, and different approaches for qualitative and quantitative assessment of spiral artery changes are discussed. The purpose of such studies is not only to obtain a better insight into mechanisms of trophoblast invasion and associated maternal tissue changes, but also to understand placental bed defects in various pregnancy complications.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Katholieke Universiteit Leuven, Leuven, Belgium

    Robert Pijnenborg, Myriam Hanssens & Lisbeth Vercruysse

  2. Departments of Obstetrics and Gynaecology, Royal Victoria Infirmary, University of Newcastle upon Tyne, Newcastle upon Tyne, UK

    Elizabeth Ball & Stephen C. Robson

  3. Department of Pathology, Royal Victoria Infirmary, University of Newcastle upon Tyne, Newcastle upon Tyne, UK

    Judith N. Bulmer

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  1. Robert Pijnenborg
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  2. Elizabeth Ball
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  3. Judith N. Bulmer
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  4. Myriam Hanssens
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  5. Stephen C. Robson
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  6. Lisbeth Vercruysse
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Editors and Affiliations

  1. Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS

    Michael J. Soares

  2. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS

    Joan S. Hunt (University Distinguished Professor, Vice Chancellor for Research) (University Distinguished Professor, Vice Chancellor for Research)

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Pijnenborg, R., Ball, E., Bulmer, J.N., Hanssens, M., Robson, S.C., Vercruysse, L. (2006). In Vivo Analysis of Trophoblast Cell Invasion in the Human. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicine™, vol 122. Humana Press. https://doi.org/10.1385/1-59259-989-3:9

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  • DOI: https://doi.org/10.1385/1-59259-989-3:9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-608-5

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