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Comparing 2-D Electrophoretic Gels Across Internet Databases

An Open Source Application

  • Protocol
The Proteomics Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

In (1–4) and in the previous edition of this book (5), we described a Web-based computer-assisted visual method called Flicker for comparing two-dimensional (2-D) protein gel images across the Internet using a Java applet. We originally used the flicker method in the GELLAB 2-D gel analysis system (6–x9 9). The applet (primarily for Web-based analysis using a Web browser) has since been converted to a Java standalone application that can run on a user’s computer. The new application also can access images from the Web but can now more easily access the user’s data on their local computer. The new version is available as open source on http://open2dprot.sourceforge.net/ Flicker, where the executable program and the source code are available. Some of the code was derived from the old Flicker applet program and some from the MicroArray Explorer (10)—an open-source data-mining tool for microarray analysis (http:// maexplorer.sourceforge.net/).

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Author information

Authors and Affiliations

  1. LECB, NCI- Frederick, Frederick, MD

    Peter F. Lemkin

  2. LECB, SAIC Frederick, Frederick, MD

    Gregory C. Thornwall

  3. LECB, CIT, NIH, Frederick, MD

    Jai Evans

Authors
  1. Peter F. Lemkin
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  2. Gregory C. Thornwall
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  3. Jai Evans
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 2005 Humana Press Inc., Totowa, NJ

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Lemkin, P.F., Thornwall, G.C., Evans, J. (2005). Comparing 2-D Electrophoretic Gels Across Internet Databases. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:279

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  • DOI: https://doi.org/10.1385/1-59259-890-0:279

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-343-5

  • Online ISBN: 978-1-59259-890-8

  • eBook Packages: Springer Protocols

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