Abstract
Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well-suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate, multiple characterizations can be done, including Western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELIS A quantitation of Aß in the brain of a transgenic mouse model of ß-amyloid deposition. The ELISA technique is described in detail in the following chapter.
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Schmidt, S.D., Jiang, Y., Nixon, R.A., Mathews, P.M. (2005). Tissue Processing Prior to Protein Analysis and Amyloid-ß Quantitation. In: Sigurdsson, E.M. (eds) Amyloid Proteins. Methods in Molecular Biology™, vol 299. Humana Press. https://doi.org/10.1385/1-59259-874-9:267
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DOI: https://doi.org/10.1385/1-59259-874-9:267
Publisher Name: Humana Press
Print ISBN: 978-1-58829-337-4
Online ISBN: 978-1-59259-874-8
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