Abstract
S100 proteins are the largest subgroup of Ca2+ binding proteins with the EF-hand structural motif. A unique feature of this protein family is that individual members are localized in specific cellular compartments. For example, various S100 proteins are expressed in very restricted regions of the hair follicle: S100A3 and S100A6 in distinct postmitotic differentiated epithelial cells and S100A4 and S100A6 in the epithelial stem cell compartments. Characterization of epithelial cells by their S100 protein expression profiles is therefore useful for a better understanding of the dynamic cellular events associated with hair follicle development and regeneration. This chapter presents our protocols for probe preparations and histochemical analyses of S100 proteins in hair follicle tissue, including simultaneous detection procedures for pulse-labeled proliferating cells.
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References
Heizmann, C. W., Fritz, G., and Schäfer, B. W. (2002) S100 proteins: structure, function and pathology. Front. Biosci. 7, d1356–d1368.
Kizawa, K., Uchiwa, H., and Murakami, U. (1996) Highly-expressed S100A3, a calciumbinding protein, in human hair cuticle. Biochim. Biophys. Acta 1312, 94–98.
Kizawa, K., Tsuchimoto, S., Hashimoto, K., and Uchiwa, H. (1998) Gene expression of mouse S100A3, a cysteine-rich calcium-binding protein, in developing hair follicle. J. Invest. Dermatol. 111, 879–886.
Wood, L., Carter, D., Mills, M., Hatzenbuhler, N., and Vogeli, G. (1991) Expression of calcyclin, a calcium-binding protein, in the keratogeous region of growing hair follicle. J. Invest. Dermatol. 96, 383–387.
Schmidt, M., Gillitzer, R., Toksoy, A., Brocker, E.-B., Rapp, U. R., Paus, R., et al. (2001) Selective expression of calcium-binding proteins S100A8 and S100A9 at distinct sites of hair follicles. J. Invest. Dermatol. 117, 748–750.
Ito, M. and Kizawa, K. (2001) Expression of calcium-binding S100 proteins A4 and A6 in Regions of the epithelial sac associated with the onset of hair follicle regeneration. J. Invest. Dermatol. 116, 956–963.
Ito, M., Kizawa K., Toyoda, M., and Morohashi, M. (2002) Label-retaining cells residing in the bulge region are directed to cell death after plucking followed by healing from the surviving hair germ. J. Invest. Dermatol. 119, 1310–1316.
Lin, M.-H., Leimeister, C., Gessler, M., and Kopan, R. (2000) Activation of the Notch pathway in the hair cortex leads to aberrant differentiation of the adjacent hair-shaft layers. Development 127, 2421–2432.
Posnett, D. N., McGrath, H., and Tam, J. P. (1988) A novel method for producing antipeptide antibodies: production of site-specific antibodies to the T cell antigen receptor β-chain. J. Biol. Chem. 263, 1719–1725.
Hopp, T. P. and Woods, K. R. (1981) Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA 78, 3824–3828.
Schägger, H., and von Jagow, G. (1989) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 40, 91–99.
Dry, F. W. (1926) The coat of the mouse (Mus musculus). J. Genet. 16, 287–340.
Chase, H. B., Rauch, H., and Smith, V. W. (1951) Critical stages of hair development and pigmentation in the mouse. Physiol. Zool. 24, 1–8.
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Kizawa, K., Ito, M. (2005). Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology™, vol 289. Humana Press. https://doi.org/10.1385/1-59259-830-7:209
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DOI: https://doi.org/10.1385/1-59259-830-7:209
Publisher Name: Humana Press
Print ISBN: 978-1-58829-267-4
Online ISBN: 978-1-59259-830-4
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