Abstract
Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5′ bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.
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© 2004 Humana Press Inc.
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Xu, B., Kastan, M.B. (2004). Analyzing Cell Cycle Checkpoints After Ionizing Radiation. In: Schönthal, A.H. (eds) Checkpoint Controls and Cancer. Methods in Molecular Biology, vol 281. Humana Press. https://doi.org/10.1385/1-59259-811-0:283
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DOI: https://doi.org/10.1385/1-59259-811-0:283
Publisher Name: Humana Press
Print ISBN: 978-1-58829-500-2
Online ISBN: 978-1-59259-811-3
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