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Opsonophagocytosis Assay Using Flow-Cytometry

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Book cover Haemophilus influenzae Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 71))

Abstract

We describe the development of a flow-cytometric-based opsonophagocytosis (OP) assay that is capable of looking at antigen-specific antibodies, that is, complement-dependent, not complement-mediated. Using the OP assay we are able to look at uptake of bacteria by neutrophils simultaneously with oxidative burst within the cell. This assay using human donor peripheral blood polymorphonuclear cells (PMNs) is rapid, reproducible, and specific. Other OP assays described use HL-60 cell lines that are technically more difficult to use and require tissue culture facilities (1).

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Author information

Authors and Affiliations

  1. Department of Paediatrics, University of Oxford, Oxford, UK

    Joyce S. Plested & Philip A. Coull

Authors
  1. Joyce S. Plested
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  2. Philip A. Coull
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Editor information

Editors and Affiliations

  1. Department of Pediatrics, John Radcliffe Hospital, Headington, Oxford, UK

    Mark A. Herbert , Derek W. Hood  & E. Richard Moxon ,  & 

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© 2003 Humana Press Inc.

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Plested, J.S., Coull, P.A. (2003). Opsonophagocytosis Assay Using Flow-Cytometry. In: Herbert, M.A., Hood, D.W., Moxon, E.R. (eds) Haemophilus influenzae Protocols. Methods in Molecular Medicine™, vol 71. Humana Press. https://doi.org/10.1385/1-59259-321-6:263

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  • DOI: https://doi.org/10.1385/1-59259-321-6:263

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-928-5

  • Online ISBN: 978-1-59259-321-7

  • eBook Packages: Springer Protocols

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