Abstract
The effectiveness of affinity chromatography relies on the ability of a molecule in solution to recognize specifically an immobilized ligand (1–3). This type of separation, unlike other chromatographic methods, uses the intrinsic biological activity of a molecule to bind to a substrate, hapten, or antigen. Principles of matrix selection, gel preparation, and coupling of ligands have been reviewed extensively by Ostrove (2). Antibody affinity chromatography has been employed to isolate antigen-specific antibodies (antibodies raised against a particular protein), hapten-specific antibodies (antipeptide antibodies, antiphosphotyrosine antibodies, anti-TNP antibodies), or species-specific immunoglobulins, or to separate crossreacting immunoglobulins from the antibody of interest (3–7).
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© 1999 Humana Press Inc., Totowa, NJ
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Kent, U.M. (1999). Purification of Antibodies Using Affinity Chromatography. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:23
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DOI: https://doi.org/10.1385/1-59259-213-9:23
Publisher Name: Humana Press
Print ISBN: 978-0-89603-813-4
Online ISBN: 978-1-59259-213-5
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