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The Bradford Method for Protein Quantitation

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Book cover The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).

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References

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© 2002 Humana Press Inc., Totowa, NJ

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Kruger, N.J. (2002). The Bradford Method for Protein Quantitation. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:15

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  • DOI: https://doi.org/10.1385/1-59259-169-8:15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-940-7

  • Online ISBN: 978-1-59259-169-5

  • eBook Packages: Springer Book Archive

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