Abstract
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).
References
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248–254.
Chial, H. J., Thompson, H. B., and Splittgerber, A. G. (1993) A spectral study of the charge forms of Coomassie Blue G. Analyt. Biochem. 209, 258–266.
Compton, S. J. and Jones, C. G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Analyt. Biochem. 151, 369–374.
Congdon, R. W., Muth, G. W., and Splittgerber, A. G. (1993) The binding interaction of Coomassie Blue with proteins. Analyt. Biochem. 213, 407–413.
Friendenauer, S. and Berlet, H. H. (1989) Sensitivity and variability of the Bradford protein assay in the presence of detergents. Analyt. Biochem. 178, 263–268.
Reade, S. M. and Northcote, D. H. (1981) Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Analyt. Biochem. 116, 53–64.
Stoscheck, C. M. (1990) Increased uniformity in the response of the Coomassie Blue protein assay to different proteins. Analyt. Biochem. 184, 111–116.
Spector, T. (1978) Refinement of the Coomassie Blue method of protein quantitation. A simple and linear spectrophotometric assay for<0.5 to 50 μg of protein. Analyt. Biochem. 86, 142–146.
Pande, S. V. and Murthy, M. S. R. (1994) A modified micro-Bradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents, and lipids. Analyt. Biochem. 220, 424–426.
Zuo, S.-S. and Lundahl, P. (2000) A micro-Bradford membrane protein assay. Analyt. Biochem. 284, 162–164.
Sedmak, J. J. and Grossberg, S. E. (1977) A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G250. Analyt. Biochem. 79, 544–552.
Peterson, G. L. (1983) Coomassie blue dye binding protein quantitation method, in Methods in Enzymology, vol. 91 (Hirs, C. H. W. and Timasheff, S. N., eds.), Academic Press, New York.
Kirazov, L. P., Venkov, L. G. and Kirazov, E. P. (1993) Comparison of the Lowry and the Bradford protein assays as applied for protein estimation of membrane-containing fractions. Analyt. Biochem. 208, 44–48.
Wilson, C. M. (1979) Studies and critique of amido black 10B, Coomassie Blue R and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis. Analyt. Biochem. 96, 263–278.
Zor, T. and Selinger, Z. (1996) Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Analyt. Biochem. 236, 302–308.
Redinbaugh, M. G. and Campbell, W. H. (1985) Adaptation of the dye-binding protein assay to microtiter plates. Analyt. Biochem. 147, 144–147.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Kruger, N.J. (2002). The Bradford Method for Protein Quantitation. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:15
Download citation
DOI: https://doi.org/10.1385/1-59259-169-8:15
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive