Abstract
Formaldehyde is a reversible cross-linker that will cross-link protein to DNA, RNA, or protein (1). Because of its high-resolution (2 Å) cross-linking, formaldehyde is a useful agent to cross-link a DNA binding protein of interest to DNA. For example, formaldehyde has been used to cross-link proteins to DNA in studies fine-mapping the distribution of particular DNA binding proteins along specific DNA sequences (1,2).
This is a preview of subscription content, log in via an institution to check access.
References
Orlando, V. (2000) Mapping chromosomal proteins by in vivo formaldehyde-crosslinked-chromatin immunoprecipitation. Trends Biochem. Sci. 25, 99–104.
Dedon, P. C., Soults, J. A., Allis, C. D., and Gorovsky, M. A. (1991) A simplified formal-dehyde fixation and immunoprecipitation technique for studying protein-DNA interactions. Analyt. Biochem. 197, 83–90.
Orlando, V., Strutt H., and Paro, R. (1997) Analysis of chromatin structure by in vivo formaldehyde crosslinking. Methods 11, 205–214.
Kadosh, D. and Struhl, K. (1998) Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Mol. Cell. Biol. 18, 5121–5127.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Spencer, V.A., Davie, J.R. (2002). Isolation of Proteins Cross-linked to DNA by Formaldehyde. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:753
Download citation
DOI: https://doi.org/10.1385/1-59259-169-8:753
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive