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Poly(Methyl Methacrylate) Nanoparticles As Vaccine Adjuvants

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Vaccine Adjuvants

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 42))

Abstract

Nanoparticles are solid particles ranging in size from 1 to 1000 nm (1 μm). They consist of macromolecular materials and can be used therapeutically or prophylactically, for example, as adjuvants in vaccines or drug carriers, in which the active principle (drug or biologically active material) is dissolved, entrapped, or encapsulated, or to which the active principle is adsorbed or chemically attached (1–3). One of the first areas of application of nanoparticles was their employment as adjuvants for vaccines. The most frequent method for the preparation of nanoparticles for this purpose was, and still is, emulsion polymerization. The polymers that can be employed for this process include poly(methyl methacrylate) (4,6), polyacrylamide (7), and poly(alkyl cyanoacrylates) (8,9). Among these polymers, poly(methyl methacrylate) proved to be by far the most optimal and suitable material (10,11). Nanoparticles made from polyacrylamide or poly(alkyl cyanoacrylate) possess the disadvantage that they either require large amounts of organic solvents and surfactants for their production, followed by laborious cleaning procedures as in the case of polyacrylamide (7), or their adjuvant effects are very poor (8,9), as with the poly(alkyl cyanoacrylates).

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References

  1. Kreuter, J. (1994) Nanoparticles, in Encyclopedia of Pharmaceutical Technology, Vol. 10 (Swarbrick, J. and Boylan, J. C., eds.), Dekker, New York, pp. 165–190.

    Google Scholar 

  2. Kreuter, J. (1983) Evaluation of nanoparticles as drug delivery systems I: preparation methods. Pharm. Acta Helv. 58, 196–209.

    CAS  Google Scholar 

  3. Kreuter, J. (1994) Nanoparticles, in Colloidal Drug Delivery Systems (Kreuter, J., ed.), Dekker, New York, pp. 219–342.

    Google Scholar 

  4. Kreuter, J. and Speiser, P. P. (1976) New adjuvants on a polymethylmethacrylate base. Infect. Immunity 13, 204–210.

    CAS  Google Scholar 

  5. Kreuter, J. and Speiser, P. P. (1976) In vitro studies of poly(methyl methacrylate) adjuvants. J. Pharm. Sci. 65, 1624–1627.

    Article  PubMed  CAS  Google Scholar 

  6. Kreuter, J., Mauler, R., Gruschkau, H., and Speiser, P. P. (1976) The use of new polymethylmethacrylate adjuvants for split influenza vaccines. Exp. CellBiol. 44, 12–19.

    CAS  Google Scholar 

  7. Birrenbach, G. and Speiser, P. P. (1976) Polymerized micelles and their use as adjuvants in immunology. J. Pharm. Sci. 65, 1763–1766.

    Article  PubMed  CAS  Google Scholar 

  8. Kreuter, J., Liehl, E., Berg, U., Soliva, M., and Speiser, P. P. (1988) Influence of hydrophobicity on the adjuvant effect of particulate polymeric adjuvants. Vaccine 6, 253–256.

    Article  PubMed  CAS  Google Scholar 

  9. Stieneker, F., Kersten, G., van Blois, L., Crommelin, D., Hem, S. L., Löwer, J., and Kreuter, J. (1995) Comparison of 24 different adjuvants for inactivated HIV-2 split whole virus as antigen in mice. Induction of titres of binding antibodies and toxicity of the formulations. Vaccine 13, 45–53.

    Article  PubMed  CAS  Google Scholar 

  10. Kreuter, J. (1995) Nanoparticles as adjuvants for vaccines, in Vaccine Design(Powell, M. F. and Newman, M. J., eds.), Plenum, New York, pp. 463–472.

    Google Scholar 

  11. Vogel, F. R. and Powell, M. F. (1995) A compendium of vaccine adjuvants and excipients: PMMA, in Vaccine Design (Powell, M. F. and Newman, M. J., eds.), Plenum, New York, p. 201.

    Google Scholar 

  12. Kreuter, J. and Haenzel, I. (1978) Mode of action of immunological adjuvants: some physicochemical factors influencing the effectivity of polyacrylic adjuvants. Infect. Immun. 19, 667–675.

    PubMed  CAS  Google Scholar 

  13. Kreuter, J. and Liehl, E. (1978) Protection induced by inactivated influenza vaccines with polymethylmethacrylate adjuvants. Med. Microbiol. Immunol. 165, 111–117.

    Article  PubMed  CAS  Google Scholar 

  14. Kreuter, J. and Liehl, E. (1981) Long-term studies of microencapsulated and adsorbed influenza vaccine nanoparticles. J. Pharm. Sci. 70, 367–371.

    Article  PubMed  CAS  Google Scholar 

  15. Kreuter, J., Berg, U., Liehl, E., Soliva, M., and Speiser, P. P. (1986) Influence of the particle size on the adjuvant effect of particulate polymeric adjuvants. Vaccine 4, 125–129.

    Article  PubMed  CAS  Google Scholar 

  16. Stieneker,F.,Kreuter,J.,and Löwer,J.(1991)High antibody titres in mice with polymethylmethacrylate nanoparticles as adjuvant for HIV vaccines.AIDS 5, 431–435.

    CAS  Google Scholar 

  17. Stieneker, F., Löwer, J., and Kreuter, J. (1993) Different kinetics of the humoral immune response to inactivated HIV-1 and HIV-2 in mice: modulation by PMMA nanoparticle adjuvant. Vaccine Res. 2, 111–118.

    CAS  Google Scholar 

  18. Berg, U. E., Kreuter, J., Speiser, P. P., and Soliva, M. (1986) Herstellung und in vitro-Prüfung von polymeren Adjuvantien für Impfstoffe. Pharm. Ind. 48, 75–79.

    CAS  Google Scholar 

  19. Kreuter, J. (1985) Poly(alkyl acrylate) nanoparticles, in Methods in Enzymology, Vol. 112, Drug and Enzyme Targeting (Widder, K. J. and Green, R., eds.), Academic, Orlando, FL, pp. 129–138.

    Chapter  Google Scholar 

  20. Kreuter, J. (1990) Large-scale production problems and manufacturing of nanoparticles, in Specialized Drug Delivery Systems (Tyle, P., ed.), Dekker, New York, pp. 257–266.

    Google Scholar 

  21. Kreuter, J., Nefzger, M., Liehl, E., Czok, R., and Voges, R. (1983) Distribution and elimination of poly(methyl methacrylate) nanoparticles after subcutaneous administration to rats. J. Pharm. Sci. 72, 1146–1149.

    Article  PubMed  CAS  Google Scholar 

  22. Charnley, J., ed. (1970) Acrylic Cement in Orthopaedic Surgery. Livingstone, Edinburgh.

    Google Scholar 

  23. Cabanela, M. E., Coventry, M. B., MacCarty, C. S., and Miller, W. E. (1972) The fate of patients with methyl methacrylate cranioplasty. J. Bone Joint Surg. 54, 278–281.

    PubMed  CAS  Google Scholar 

  24. Kreuter, J., Täuber, U., and Illi, V. (1979) Distribution and elimination of poly(methyl-2-14C-methacrylate) nanoparticle radioactivity after injection in rats and mice. J. Pharm. Sci. 68, 1443–1447.

    Article  PubMed  CAS  Google Scholar 

  25. Schindler, A., Jeffcoat, R., Kimmel, G. L., Pitt, C. G., Wall, M. E., and Zweidinger, R. (1977) Biodegradable polymers for sustained drug delivery, in Contemporary Topics in Polymer Science, Vol.2 (Pearce, E. M. and Schaefgen, J. R., eds.), Plenum, New York, pp. 251–289.

    Google Scholar 

  26. Nefzger, M., Kreuter, J., Voges, R., Liehl, E., and Czok, R. (1984) Distribution and elimination of polymethyl methacrylate nanoparticles after peroral administration to rats. J. Pharm. Sci. 73, 1309–1311.

    Article  PubMed  CAS  Google Scholar 

  27. Fikentscher, H., Gerrens, H., and Schuller, H. (1960) Emulsionspolymerisation und Kunststoff-Latices. Angew. Chem. 72, 856–864.

    Article  CAS  Google Scholar 

  28. Van der Hoff, B. M. E. (1960) On the mechanism of emulsion polymerization of styrene. II. polymerization in soap solutions below and above the critical micelle concentration and some remarks on the kinetics of emulsion polymerization. J. Polymer Sci. 44, 241–259.

    Article  Google Scholar 

  29. Roe, C. P. (1968) Surface chemistry aspects of emulsion polymerization. Ind. Eng. Chem. 60, 20–33.

    Article  CAS  Google Scholar 

  30. Robb, I. D. (1969) Determination of the number of particles/unit volume of latex during the emulsion polymerization of styrene. J. Polymer. Sci.,Part A-I7, 417–427.

    Google Scholar 

  31. Fitch, R. M., Prenosil, M. B., and Sprick (1969) The mechanism of particle formation in polymer hydrosols. I. kinetics of aqueous polymerization of methyl methacrylate. J. Polymer Sci., Part C 27, 95–118. 119

    Google Scholar 

  32. Fitch, R. M. and Tsoi, C. (1970) Polymer colloids: particle formation in nonmicellar systems. J. Polymer Sci., Polymer Lett. 8, 703–710.

    Article  CAS  Google Scholar 

  33. Fitch, R. M. (1973) The homogeneous nucleation of polymer colloids. Brit. Polym. J. 5, 467–483.

    Article  CAS  Google Scholar 

  34. Fitch, R. M., Prenosil, M. B., and Sprick, K. J. (1966) The mechanism of particle formation in polymer hydrosols. I. kinetics of aqueous polymerization of methyl methacrylate. Amer. Chem. Soc., Polymer Preprints 7, 707–724.

    CAS  Google Scholar 

  35. Kreuter, J. (1982) On the mechanism of termination in heterogeneous polymerization. J. Polymer Sci., Polymer Lett. 20, 543–545.

    Article  CAS  Google Scholar 

  36. Fiquet-Fayard, F. (1959) Polymérization radiochimique du méthacrylate de méthyle (M.A.M.) en solution aqueuse. J. Chim. Phys. 56, 692–698.

    CAS  Google Scholar 

  37. Acres, G. J. K. and Dalton, F. L. (1963) On Co60 gamma ray-initiated emulsion polymerization. J. Polymer Sci. A 1, 3009–3020.

    CAS  Google Scholar 

  38. Kreuter, J. and Zehnder, H. J. (1978) The use of 60Co-γ-irradiation for the production of vaccines. Rad. Eff. 35, 161–166.

    Article  CAS  Google Scholar 

  39. Kreuter, J. (1983) Physicochemical characterization of polyacrylic nanoparticles. Int. J. Pharm. 14, 43–58.

    Article  CAS  Google Scholar 

  40. Bentele, V., Berg, U. E., and Kreuter, J. (1983) Molecular weights of poly(methyl methacrylate) nanoparticles. Int. J. Pharm. 13, 109–113.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institut für Pharmazeutische Technologie, Johann Wolfgang Goethe-Universität, Biozentrum, Frankfurt am Main, Germany

    Jörg Kreuter

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  1. Jörg Kreuter
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Editors and Affiliations

  1. Chiron Corporation, Emeryville, CA

    Derek T. O’Hagan

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© 2000 Humana Press Inc.

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Kreuter, J. (2000). Poly(Methyl Methacrylate) Nanoparticles As Vaccine Adjuvants. In: O’Hagan, D.T. (eds) Vaccine Adjuvants. Methods in Molecular Medicine™, vol 42. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-083-7:105

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  • DOI: https://doi.org/10.1385/1-59259-083-7:105

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-735-9

  • Online ISBN: 978-1-59259-083-4

  • eBook Packages: Springer Protocols

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