Abstract
Dendritic cells (DC) that reside in tissues such as the epidermis, lung, or spleen, and those that circulate in the blood, are functionally and phenotypi-cally immature (1,2). In this immature or precursor state, DCs can process protein antigens (Ags), and phagocytose and process particulate matter efficiently; however, they are poor stimulators of T-lymphocytes. As DCs migrate from the epidermis to the lymphoid organs, several phenotypic and functional changes occur: they acquire several co-stimulatory molecules; upregulate major histocompatibility complex class II (MHC-II), DEC-205 Ag receptor, and granule Ag M342; become less phagocytic; lose their protein-processing capabilities; and eventually become potent stimulators of T-lymphocytes. Similar to their in vivo properties, DCs freshly isolated from the blood, skin, or spleen are functionally and phenotypically immature, but after 1-2 d in culture,
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Ahuja, S. (2000). In Vitro Generation of Functional Human and Murine Dendritic Cells. In: Solheim, J.C. (eds) Antigen Processing and Presentation Protocols. Methods in Molecular Biology, vol 156. Humana Press. https://doi.org/10.1385/1-59259-062-4:67
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DOI: https://doi.org/10.1385/1-59259-062-4:67
Publisher Name: Humana Press
Print ISBN: 978-0-89603-745-8
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