Abstract
Vaccinia vims-mediated gene transfer is a powerful technique to test the function of individual receptors in natural killer (NK) cells. By transient expression of genes encoding NK receptors, one can obtain high levels of protein synthesis, while maintaining NK cell functions. The vaccinia virus expression system is useful for the analysis of wild-type and mutant forms of NK receptors and for the study of structure-function relationships as they relate to receptor-ligand interactions and signaling events.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Earl P. L. and Moss B. (1988) Generation of recombinant vaccinia viruses, in Current Protocols in Molecular Biology, (Ausubel F. M., Brent R., Kingston R. E., et al., eds.), Wiley & Sons New York, pp. 16.17.1–16.17.16.
Gong J. H., Maki G., and Klingemann H. G. (1994) Characterization of a human cell line (NK-92) with phenotypical and functional characteristics of activated natural killer cells. Leukemia 8, 652–658.
Kornbluth J., Flomenberg N., and Dupont B. (1982) Cell surf ace phenotype of a cloned line of human natural killer cells. J. Immunol 129, 2831–2837.
Wagtmann N., Rajagopalan S., Winter C. C, Peruzzi M., and Long E. 0.(1995) Killer cell inhibitory receptors specific for HLA-C and HLA-B identified by direct binding and by functional transfer. Immunity 3, 801–809.
Rajagopalan S., Winter C. C, Wagtmann N., and Long E. O. (1995) The Ig-related killer cell inhibitory receptor binds zinc and requires zinc for recognition of HLA-C on target cells. J. Immunol. 155, 4143–4146.
Winter C. C, Gumperz J. E., Parham P., Long E. O., and Wagtmann N.(1998) Direct binding and functional transfer of natural killer cell inhibitory receptors reveal novel patterns of HLA-C allotype recognition. J. Immunol.,in press.
Rojo S., Burshtyn D. N., Long E. 0., and Wagtmann N. (1997) Type I transmembrane receptor with inhibitory function in mouse mast cells and NK cells. J.Immunol. 158, 9–12.
Gupta N., Scharenberg A. M., Burshtyn D. N., Wagtmann N., Lioubin M. N, RohrSchneider L. R., Kinet J. P., and Long E. 0. (1997) Negative signaling pathways of the killer cell inhibitory receptor andFcgammaRIIbl require distinct phosphatases. J. Exp. Med. 186, 473–478.
Rajagopalan S. and Long E. 0. (1998) Zinc Bound to the Killer Cell Inhibitory Receptor Modulates the Negative Signal in Human Natural Killer Cells. J. Immunol 161, 1299–1305.
Burshtyn D. N., Scharenberg A. M., Wagtmann N., Rajagopalan S., Berrada K.. Yif T., Kinet J. P., and I-ong E. O. (1996) Recruitment of tyrosine phosphatase HCP by the killer cell inhibitor receptor. Immunity 4, 77–85.
Binstadt B. A., Brumbaugh K. M., Dick C. J., Scharenberg A. M., Williams B. L.; Colonna M., Lanier L. L., Kinet J. P., Abraham R. T., and Leibson P. J. (1996) Sequential involvement of Lck and SHP-1 with MHC-recognizing receptors on NK cells inhibits FcR-initiated tyrosine kinase activation. Immunity 5, 629–638.
Ting A. T., Dick C. J., Schoon R. A., Karnitz L. M., Abraham R. T., and Leibson P. J. (1995) Interaction between lck and syk family tyrosine kinases in Fcgamma receptor-initiated activation of natural killer cells. J. Biol. Chem. 270. 16,415–16,421.
Brumbaugh K. M., Binstadt B. A., Billadeau D. D., Schoon R. A., Dick C. J.; Ten R. M., and Leibson P. J. (1997) Functional role for Syk tyrosine kinase in natural killer cell-mediated natural cytotoxicity. J. Exp. Med. 186, 1965–1974.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc.
About this protocol
Cite this protocol
Rajagopalan, S., Long, E.O. (1999). Use of Vaccinia Virus for Functional Gene Transfer in Natural Killer Cells. In: Campbell, K.S., Colonna, M. (eds) Natural Killer Cell Protocols. Methods in Molecular Biology, vol 121. Humana Press. https://doi.org/10.1385/1-59259-044-6:265
Download citation
DOI: https://doi.org/10.1385/1-59259-044-6:265
Publisher Name: Humana Press
Print ISBN: 978-0-89603-683-3
Online ISBN: 978-1-59259-044-5
eBook Packages: Springer Protocols