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Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome

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Book cover Bioluminescence Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 102))

Abstract

Transcriptional reporter systems have been extensively used to measure gene expression. Facile measurement of a reporter signal enables the study of gene expression in cases where protein product quantification is problematic. Reporters, such as /gbgalactostdase, provide convenient chromogenic assays (1). More recently, bioluminescence has been developed as a monitormg system (1). Reporter gene systems may be located extrachromosomally or integrated into the chromosome.

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References

  1. Silhavy, T. J., Berman, M., and Enquist, L. (1984) Experiments with Gene Fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  2. Stewart, G. S. A. B. and Williams, P. (1992) lux genes and the applications of bacterial bioluminescence. J Gen. Mcrobzol. 138, 1289–1300

    CAS  Google Scholar 

  3. Balbas, P., Alexeyev, M., Shokolenko, I., Bolivar, F, and Valle, F. (1996) A pBRINT family of plasmids for integration of cloned DNA into the Escherzchia colt chromosome. Gene 172, 65–69.

    Article  PubMed  CAS  Google Scholar 

  4. Vollmer, A. C., Belkin, S., Smulski, D. R., Dyk, T V., and LaRossa, R. A. (1996) Detection of DNA damage using Eschemhza colt carrymg recA/ls.:lux, uvrA::lux or alkA/rs lux reporter plasmids. Appl Envwon Microbzol 63, 2566–2571.

    Google Scholar 

  5. Nishimura, A., Morita, M., Nishimura, Y., and Sugino, Y. (1990) A rapid and highly efficient method for preparation of competent Escherzchza colt cells. Nucleic Acids Res 18, 6169

    Article  PubMed  CAS  Google Scholar 

  6. LaRossa, R. A. (1996) Mutant selections lmking physiology, inhibitors, and genotype, in Escherzchla COB and Salmonella Nerdhardt, F.C., Curttss, R, III, Ingraham, J L, Lm, E C. C., Low, K. B., Magasamk, B., Reznikoff, W.S, Riley, M, Schaechter, M., and Umbarger, H.E.,eds), ASM, Washington, DC, pp 2527–2587

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© 1998 Humana Press Inc., Totowa, NJ

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David, A.E. (1998). Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome. In: LaRossa, R.A. (eds) Bioluminescence Methods and Protocols. Methods in Molecular Biology™, vol 102. Humana Press. https://doi.org/10.1385/0-89603-520-4:97

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  • DOI: https://doi.org/10.1385/0-89603-520-4:97

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-520-1

  • Online ISBN: 978-1-59259-577-8

  • eBook Packages: Springer Protocols

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